Molecular evidence for horizontal transmission of chelonid alphaherpesvirus 5 at green turtle (Chelonia mydas) foraging grounds in Queensland, Australia

Autoři: K. Jones aff001;  G. Burgess aff001;  A. M. Budd aff002;  R. Huerlimann aff002;  N. Mashkour aff001;  E. Ariel aff001
Působiště autorů: College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia aff001;  Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, Queensland, Australia aff002;  Centre for Tropical Water and Aquatic Ecosystem Research, James Cook University, Townsville, Queensland, Australia aff003
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0227268


Fibropapillomatosis (FP) is a marine turtle disease recognised by benign tumours on the skin, eyes, shell, oral cavity and/or viscera. Despite being a globally distributed disease that affects an endangered species, research on FP and its likely causative agent chelonid alphaherpesvirus 5 (ChHV5) in Australia is limited. Here we present improved molecular assays developed for detection of ChHV5, in combination with a robust molecular and phylogenetic analysis of ChHV5 variants. This approach utilised a multi-gene assay to detect ChHV5 in all FP tumors sampled from 62 marine turtles found at six foraging grounds along the Great Barrier Reef. Six distinct variants of ChHV5 were identified and the distribution of these variants was associated with host foraging ground. Conversely, no association between host genetic origin and ChHV5 viral variant was found. Together this evidence supports the hypothesis that marine turtles undergo horizontal transmission of ChHV5 at foraging grounds and are unlikely to be contracting the disease at rookeries, either during mating or vertically from parent to offspring.

Klíčová slova:

DNA sequence analysis – Foraging – Haplotypes – Phylogenetic analysis – Sequence alignment – Sequence analysis – Sequence databases – Turtles


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