Centromeres of Cucumis melo L. comprise Cmcent and two novel repeats, CmSat162 and CmSat189
Autoři:
Agus Budi Setiawan aff001; Chee How Teo aff002; Shinji Kikuchi aff001; Hidenori Sassa aff001; Kenji Kato aff003; Takato Koba aff001
Působiště autorů:
Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba University, Matsudo, Chiba, Japan
aff001; Center for Research in Biotechnology for Agriculture, University of Malaya, Kuala Lumpur, Malaysia
aff002; Graduate School of Environmental and Life Science, Okayama University, Kita-ku, Okayama, Japan
aff003
Vyšlo v časopise:
PLoS ONE 15(1)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0227578
Souhrn
Centromeres are prerequisite for accurate segregation and are landmarks of primary constrictions of metaphase chromosomes in eukaryotes. In melon, high-copy-number satellite DNAs (SatDNAs) were found at various chromosomal locations such as centromeric, pericentromeric, and subtelomeric regions. In the present study, utilizing the published draft genome sequence of melon, two new SatDNAs (CmSat162 and CmSat189) of melon were identified and their chromosomal distributions were confirmed using fluorescence in situ hybridization. DNA probes prepared from these SatDNAs were successfully hybridized to melon somatic and meiotic chromosomes. CmSat162 was located on 12 pairs of melon chromosomes and co-localized with the centromeric repeat, Cmcent, at the centromeric regions. In contrast, CmSat189 was found to be located not only on centromeric regions but also on specific regions of the chromosomes, allowing the characterization of individual chromosomes of melon. It was also shown that these SatDNAs were transcribed in melon. These results suggest that CmSat162 and CmSat189 might have some functions at the centromeric regions.
Klíčová slova:
Centromeres – DNA sequence analysis – Melons – Repeated sequences – Sequence analysis – Tandem repeats – Tandem repeat sequence analysis – Satellite DNA
Zdroje
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