Analysis of the Rdr1 gene family in different Rosaceae genomes reveals an origin of an R-gene cluster after the split of Rubeae within the Rosoideae subfamily

Autoři: Ina Menz aff001;  Deepika Lakhwani aff002;  Jérémy Clotault aff002;  Marcus Linde aff001;  Fabrice Foucher aff002;  Thomas Debener aff001
Působiště autorů: Institute for Plant Genetics, Leibniz Universität Hannover, Hannover, Germany aff001;  IRHS, Agrocampus-Ouest, INRA, Université d’Angers, Beaucouzé, France aff002
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0227428


The Rdr1 gene confers resistance to black spot in roses and belongs to a large TNL gene family, which is organized in two major clusters at the distal end of chromosome 1. We used the recently available chromosome scale assemblies for the R. chinensis ‘Old Blush’ genome, re-sequencing data for nine rose species and genome data for Fragaria, Rubus, Malus and Prunus to identify Rdr1 homologs from different taxa within Rosaceae. Members of the Rdr1 gene family are organized into two major clusters in R. chinensis and at a syntenic location in the Fragaria genome. Phylogenetic analysis indicates that the two clusters existed prior to the split of Rosa and Fragaria and that one cluster has a more recent origin than the other. Genes belonging to cluster 2, such as the functional Rdr1 gene muRdr1A, were subject to a faster evolution than genes from cluster 1. As no Rdr1 homologs were found in syntenic positions for Prunus persica, Malus x domestica and Rubus occidentalis, a translocation of the Rdr1 clusters to the current positions probably happened after the Rubeae split from other groups within the Rosoideae approximately 70–80 million years ago during the Cretaceous period.

Klíčová slova:

Amino acid sequence analysis – Genome analysis – Genomic medicine – Homologous chromosomes – Phylogenetic analysis – Plant genomics – Sequence alignment – Roses


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