Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis

Autoři: Élcio Leal aff001;  Ruiying Liang aff001;  Qi Liu aff001;  Fabiola Villanova aff002;  Lijun Shi aff001;  Lin Liang aff001;  Jinxiang Li aff001;  Steven S. Witkin aff004;  Shangjin Cui aff001
Působiště autorů: Chinese Academy of Agricultural Sciences, Institute of Animal Sciences, Beijing, China aff001;  Federal University of Pará, Belém, Pará, Brazil aff002;  Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology, Ministry of Agriculture, Beijing, China aff003;  Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, NY, United States of America aff004;  Institute of Tropical Medicine, Sao Paulo, Brazil aff005
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0227705


Protoparvoviruses, widespread among cats and wild animals, are responsible for leukopenia. Feline panleukopenia virus (FPLV) in domestic cats is genetically diverse and some strains may differ from those used for vaccination. The presence of FPLV in two domestic cats from Hebei Province in China was identified by polymerase chain reaction. Samples from these animals were used to isolate FPLV strains in CRFK cells for genome sequencing. Phylogenetic analysis was performed to compare our isolates with available sequences of FPLV, mink parvovirus (MEV) and canine parvovirus (CPV). The isolated strains were closely related to strains of FPLV/MEV isolated in the 1960s. Our analysis also revealed that the evolutionary history of FPLV and MEV is characterized by local adaptations in the Vp2 gene. Thus, it is likely that new FPLV strains are emerging to evade the anti-FPLV immune response.

Klíčová slova:

Amino acid sequence analysis – Carnivory – DNA sequence analysis – Domestic animals – Mammalian genomics – Phylogenetic analysis – Polymerase chain reaction – Parvoviruses


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