HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system


Autoři: Woottichai Khamduang aff001;  Ampika Kaewbundit aff003;  Amonrat Duangmano aff003;  Sayamon Hongjaisee aff004;  Virat Klinbuayaem aff005;  Guttiga Halue aff006;  Apichat Chutanunta aff007;  Wasna Sirirungsi aff001;  Gonzague Jourdain aff001;  Nicole Ngo-Giang-Huong aff001
Působiště autorů: Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiangmai, Thailand aff001;  Infectious Diseases Research Unit, Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiangmai, Thailand aff002;  Institut de Recherche pour le Développement (IRD), Chiangmai, Thailand aff003;  Research Institute for Health Sciences, Chiang Mai University, Chiangmai, Thailand aff004;  Sanpatong hospital, Chiangmai, Thailand aff005;  Phayao hospital, Phayao, Thailand aff006;  Samut Sakorn hospital, Samut Sakorn, Thailand aff007;  Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, United States of America aff008
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0227929

Souhrn

World Health Organization recommends using dried blood spots (DBS) for HIV RNA viral load (VL) measurement whenever plasma processing is not convenient or feasible. DBS collected from 80 treatment-naïve HIV-infected patients presenting in three hospitals of two different regions of Thailand were shipped to a central laboratory along with corresponding plasma specimens. Viral load was measured in both DBS and plasma using the Abbott m2000 system. HIV RNA levels were strongly correlated (r = 0.94) with a mean of differences of 0.23 log10 copies/mL. Using the 1,000 copies/mL cut-off, the sensitivity of DBS was 97% (95%CI, 91–100%) and specificity was 75% (95%CI, 19–99%). DBS are useful to scale-up HIV RNA VL testing in settings with limited access to VL testing.

Klíčová slova:

Antiretroviral therapy – Blood – Blood plasma – HIV – HIV epidemiology – HIV prevention – Thailand – Viral load


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2020 Číslo 1