Effects of dietary supplementation with a microalga (Schizochytrium sp.) on the hemato-immunological, and intestinal histological parameters and gut microbiota of Nile tilapia in net cages


Autoři: Felipe Pinheiro de Souza aff001;  Ed Christian Suzuki de Lima aff001;  Angela Maria Urrea-Rojas aff001;  Suelen Aparecida Suphoronski aff002;  César Toshio Facimoto aff002;  Jailton da Silva Bezerra Júnior aff003;  Thalita Evani Silva de Oliveira aff002;  Ulisses de Pádua Pereira aff002;  Giovana Wingeter Di Santis aff002;  Carlos Antonio Lopes de Oliveira aff003;  Nelson Mauricio Lopera-Barrero aff001
Působiště autorů: Department of Animal Science, State University of Londrina, Londrina, Parana, Brazil aff001;  Department of Preventive Veterinary Medicine, State University of Londrina, Londrina, Parana, Brazil aff002;  Department of Animal Science, State University of Maringa, Maringa, Parana, Brazil aff003
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226977

Souhrn

Nutritional improvements in intensive aquaculture production systems is necessary for the reduction of stress, maximum utilization of nutritional components, and expression of the genetic potential of fish. The objective of this study was to evaluate the hemato-immunological, and histological parameters and gut microbiota of Nile tilapia fed with the microalga Schizochytrium sp. Males of Nile tilapia were distributed among eight net cages (6 m3), and fed for 105 days with two diets: control (CON), without Schizochytrium sp., and supplemented (SUP), with 1.2% Schizochytrium sp. in the diet. The final weight, mortality, hematocrit, total erythrocyte count (RBC), hemoglobin, hematimetric indices, white blood cell count (WBC), total protein, and serum lysozyme were measured. Alterations in intestinal morphology were evaluated. The gut microbiota was evaluated with next-generation sequencing. No significant differences (p>0.05) were found in the final weight and mortality between diets. Regarding the hematological parameters, a difference (p<0.05) was detected only in RBC, with there being lower values in the SUP, although this group also showed a tendency toward having an increased mean corpuscular hemoglobin level. There were no differences (p>0.05) in total protein and serum lysozyme concentrations or in WBCs between diets, except for lymphocytes, which presented lower values (p<0.05) in the SUP, suggesting immunomodulation by the polyunsaturated fatty acids present in the microalga. There was no difference (p>0.05) in the intestinal morphology between diets. Metagenomic data indicated greater richness (represented by the Chao index) and a higher abundance of the bacterial phylum Firmicutes in the gut microbiota of the tilapia fed with the SUP diet, demonstrating that the digestion and use of the components of the microalga could influence the microbial community. The results indicated that the microalga had modulatory effects on blood cells and the intestinal microbiota, without affecting the structure and integrity of the intestinal villi.

Klíčová slova:

Algae – Bacteria – Diet – Fish physiology – Gastrointestinal tract – Gut bacteria – Lysozyme – Serum proteins


Zdroje

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