#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Evaluation of liposomal ciprofloxacin formulations in a murine model of anthrax


Autoři: Chad W. Stratilo aff001;  Scott Jager aff001;  Melissa Crichton aff001;  James D. Blanchard aff002
Působiště autorů: Bio Threat Defence Section, Suffield Research Centre, Defence Research and Development Canada, Ralston, Alberta, Canada aff001;  Aradigm Corporation, Hayward, California, United States of America aff002
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0228162

Souhrn

The in vivo efficacy of liposomal encapsulated ciprofloxacin in two formulations, lipoquin and apulmiq, were evaluated against the causative agent of anthrax, Bacillus anthracis. Liposomal encapsulated ciprofloxacin is attractive as a therapy since it allows for once daily dosing and achieves higher concentrations of the antibiotic at the site of initial mucosal entry but lower systemic drug concentrations. The in vivo efficacy of lipoquin and apulmiq delivered by intranasal instillation was studied at different doses and schedules in both a post exposure prophylaxis (PEP) therapy model and in a delayed treatment model of murine inhalational anthrax. In the mouse model of infection, the survival curves for all treatment cohorts differed significantly from the vehicle control. Ciprofloxacin, lipoquin and apulmiq provided a high level of protection (87–90%) after 7 days of therapy when administered within 24 hours of exposure. Reducing therapy to only three days still provided protection of 60–87%, if therapy was provided within 24 hours of exposure. If treatment was initiated 48 hours after exposure the survival rate was reduced to 46–65%. These studies suggest that lipoquin and apulmiq may be attractive therapies as PEP and as part of a treatment cocktail for B. anthracis.

Klíčová slova:

Anthrax – Antibiotics – Bacillus anthracis – Bacterial spores – Inhalation – Mouse models – Post-exposure prophylaxis – Prophylaxis


Zdroje

1. Riedel S. Anthrax: a continuing concern in the era of bioterrorism. Proc (Bayl Univ Med Cent). 2005;18(3):234–43. Epub 2005/10/04. doi: 10.1080/08998280.2005.11928074 16200179.

2. Inglesby TV, Henderson DA, Bartlett JG, Ascher MS, Eitzen E, Friedlander AM, et al. Anthrax as a biological weapon: medical and public health management. Working Group on Civilian Biodefense. JAMA. 1999;281(18):1735–45. Epub 1999/05/18. doi: 10.1001/jama.281.18.1735 10328075.

3. Holty JE, Kim RY, Bravata DM. Anthrax: a systematic review of atypical presentations. Ann Emerg Med. 2006;48(2):200–11. Epub 2006/07/22. doi: 10.1016/j.annemergmed.2005.11.035 16857469.

4. Guidi-Rontani C. The alveolar macrophage: the Trojan horse of Bacillus anthracis. Trends Microbiol. 2002;10(9):405–9. Epub 2002/09/10. doi: 10.1016/s0966-842x(02)02422-8 12217505.

5. Cleret A, Quesnel-Hellmann A, Vallon-Eberhard A, Verrier B, Jung S, Vidal D, et al. Lung dendritic cells rapidly mediate anthrax spore entry through the pulmonary route. J Immunol. 2007;178(12):7994–8001. Epub 2007/06/06. doi: 10.4049/jimmunol.178.12.7994 17548636.

6. Dixon TC, Meselson M, Guillemin J, Hanna PC. Anthrax. N Engl J Med. 1999;341(11):815–26. Epub 1999/09/09. doi: 10.1056/NEJM199909093411107 10477781.

7. Dixon TC, Fadl AA, Koehler TM, Swanson JA, Hanna PC. Early Bacillus anthracis-macrophage interactions: intracellular survival survival and escape. Cell Microbiol. 2000;2(6):453–63. Epub 2001/02/24. doi: 10.1046/j.1462-5822.2000.00067.x 11207600.

8. Weiner ZP, Glomski IJ. Updating perspectives on the initiation of Bacillus anthracis growth and dissemination through its host. Infect Immun. 2012;80(5):1626–33. Epub 2012/02/23. doi: 10.1128/IAI.06061-11 22354031.

9. Booth JL, Duggan ES, Patel VI, Langer M, Wu W, Braun A, et al. Bacillus anthracis spore movement does not require a carrier cell and is not affected by lethal toxin in human lung models. Microbes Infect. 2016;18(10):615–26. Epub 2016/06/21. doi: 10.1016/j.micinf.2016.06.004 27320392.

10. Jernigan JA, Stephens DS, Ashford DA, Omenaca C, Topiel MS, Galbraith M, et al. Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States. Emerg Infect Dis. 2001;7(6):933–44. Epub 2001/12/19. 11747719.

11. Heine HS, Purcell BK, Bassett J, Miller L, Goldstein BP. Activity of dalbavancin against Bacillus anthracis in vitro and in a mouse inhalation anthrax model. Antimicrob Agents Chemother. 2010;54(3):991–6. Epub 2010/01/06. doi: 10.1128/AAC.00820-09 20047912.

12. Peterson JW, Moen ST, Healy D, Pawlik JE, Taormina J, Hardcastle J, et al. Protection Afforded by Fluoroquinolones in Animal Models of Respiratory Infections with Bacillus anthracis, Yersinia pestis, and Francisella tularensis. The open microbiology journal. 2010;4:34–46. Epub 2010/12/04. doi: 10.2174/1874285801004010034 21127743.

13. Weiss S, Kobiler D, Levy H, Pass A, Ophir Y, Rothschild N, et al. Antibiotics cure anthrax in animal models. Antimicrob Agents Chemother. 2011;55(4):1533–42. Epub 2011/01/26. doi: 10.1128/AAC.01689-10 21263056.

14. Vietri NJ, Purcell BK, Lawler JV, Leffel EK, Rico P, Gamble CS, et al. Short-course postexposure antibiotic prophylaxis combined with vaccination protects against experimental inhalational anthrax. Proc Natl Acad Sci U S A. 2006;103(20):7813–6. Epub 2006/05/05. doi: 10.1073/pnas.0602748103 16672361.

15. Weiss S, Altboum Z, Glinert I, Schlomovitz J, Sittner A, Bar-David E, et al. Efficacy of Single and Combined Antibiotic Treatments of Anthrax in Rabbits. Antimicrob Agents Chemother. 2015;59(12):7497–503. Epub 2015/09/24. doi: 10.1128/AAC.01376-15 26392505.

16. Migone TS, Subramanian GM, Zhong J, Healey LM, Corey A, Devalaraja M, et al. Raxibacumab for the treatment of inhalational anthrax. N Engl J Med. 2009;361(2):135–44. Epub 2009/07/10. doi: 10.1056/NEJMoa0810603 19587338.

17. Corey A, Migone TS, Bolmer S, Fiscella M, Ward C, Chen C, et al. Bacillus anthracis protective antigen kinetics in inhalation spore-challenged untreated or levofloxacin/ raxibacumab-treated New Zealand white rabbits. Toxins. 2013;5(1):120–38. Epub 2013/01/25. doi: 10.3390/toxins5010120 23344456.

18. Hendricks KA, Wright ME, Shadomy SV, Bradley JS, Morrow MG, Pavia AT, et al. Centers for disease control and prevention expert panel meetings on prevention and treatment of anthrax in adults. Emerg Infect Dis. 2014;20(2). Epub 2014/01/23. doi: 10.3201/eid2002.130687 24447897.

19. Hagerman JK, Hancock KE, Klepser ME. Aerosolised antibiotics: a critical appraisal of their use. Expert opinion on drug delivery. 2006;3(1):71–86. Epub 2005/12/24. doi: 10.1517/17425247.3.1.71 16370941.

20. Velkov T, Abdul Rahim N, Zhou QT, Chan HK, Li J. Inhaled anti-infective chemotherapy for respiratory tract infections: successes, challenges and the road ahead. Adv Drug Deliv Rev. 2015;85:65–82. Epub 2014/12/03. doi: 10.1016/j.addr.2014.11.004 25446140.

21. Falagas ME, Trigkidis KK, Vardakas KZ. Inhaled antibiotics beyond aminoglycosides, polymyxins and aztreonam: A systematic review. Int J Antimicrob Agents. 2015;45(3):221–33. Epub 2014/12/24. doi: 10.1016/j.ijantimicag.2014.10.008 25533880.

22. Wood GC, Swanson JM. Aerosolised antibacterials for the prevention and treatment of hospital-acquired pneumonia. Drugs. 2007;67(6):903–14. Epub 2007/04/13. doi: 10.2165/00003495-200767060-00006 17428107.

23. Cipolla D, Blanchard J, Gonda I. Development of Liposomal Ciprofloxacin to Treat Lung Infections. Pharmaceutics. 2016;8(1). Epub 2016/03/05. doi: 10.3390/pharmaceutics8010006 26938551.

24. Serisier DJ, Bilton D, De Soyza A, Thompson PJ, Kolbe J, Greville HW, et al. Inhaled, dual release liposomal ciprofloxacin in non-cystic fibrosis bronchiectasis (ORBIT-2): a randomised, double-blind, placebo-controlled trial. Thorax. 2013;68(9):812–7. Epub 2013/05/18. doi: 10.1136/thoraxjnl-2013-203207 23681906.

25. Serisier DJ. Inhaled antibiotics for lower respiratory tract infections: focus on ciprofloxacin. Drugs Today (Barc). 2012;48(5):339–51. Epub 2012/05/31. doi: 10.1358/dot.2012.48.5.1789474 22645722.

26. Bruinenberg P, Serisier D, Cipolla D, Blanchard J. Safety, Tolerability and Pharmacokinetics of Novel Liposomal Ciprofloxacin formulations for inhalation in Healthy volunteers and Non-Cystic bronchiectasis Patients. Am J Resp Crit Care. 2010;181.

27. Bruinenberg P, Serisier D, Cipolla D, Blanchard J. Safety, Tolerability, Pharmacokinetics & Antimicrobial Activity of Inhaled Liposomal Ciprofloxacin Formulations in Humans. Pediatr Pulm. 2010:354.

28. Haworth C, Wanner A, Froehlich J, O’Neal T, Davis A, Gonda I, et al. Inhaled Liposomal Ciprofloxacin In Patients With Bronchiectasis And Chronic Pseudomonas AerugINOSa Infection: Results From Two Parallel Phase Iii Trials (orbit-3 And-4). Am J Resp Crit Care. 2017;195.

29. Bilton D, Bruinenberg P, Otulana B, Morishige R, Blanchard J, DeSoyza A, et al. Inhaled Liposomal Ciprofloxacin Hydrochloride Significantly Reduces Sputum Pseudomonas Aeruginosa Density in CF and Non-CF Bronchiectasis. Am J Resp Crit Care. 2009;179.

30. Chono S, Tanino T, Seki T, Morimoto K. Efficient drug delivery to alveolar macrophages and lung epithelial lining fluid following pulmonary administration of liposomal ciprofloxacin in rats with pneumonia and estimation of its antibacterial effects. Drug development and industrial pharmacy. 2008;34(10):1090–6. Epub 2008/09/09. doi: 10.1080/03639040801958421 18777242.

31. Magallanes M, Dijkstra J, Fierer J. Liposome-incorporated ciprofloxacin in treatment of murine salmonellosis. Antimicrob Agents Chemother. 1993;37(11):2293–7. Epub 1993/11/01. doi: 10.1128/aac.37.11.2293 8285608.

32. Webb MS, Boman NL, Wiseman DJ, Saxon D, Sutton K, Wong KF, et al. Antibacterial efficacy against an in vivo Salmonella typhimurium infection model and pharmacokinetics of a liposomal ciprofloxacin formulation. Antimicrob Agents Chemother. 1998;42(1):45–52. Epub 1998/02/04. 9449259.

33. Blanchard JD, Elias V, Cipolla D, Gonda I, Bermudez LE. Effective Treatment of Mycobacterium avium subsp. hominissuis and Mycobacterium abscessus Species Infections in Macrophages, Biofilm, and Mice by Using Liposomal Ciprofloxacin. Antimicrob Agents Chemother. 2018;62(10). Epub 2018/07/18. doi: 10.1128/AAC.00440-18 30012773.

34. Hamblin KA, Wong JP, Blanchard JD, Atkins HS. The potential of liposome-encapsulated ciprofloxacin as a tularemia therapy. Frontiers in cellular and infection microbiology. 2014;4:79. Epub 2014/07/06. doi: 10.3389/fcimb.2014.00079 24995163.

35. Norville IH, Hatch GJ, Bewley KR, Atkinson DJ, Hamblin KA, Blanchard JD, et al. Efficacy of liposome-encapsulated ciprofloxacin in a murine model of Q fever. Antimicrob Agents Chemother. 2014;58(9):5510–8. Epub 2014/07/09. doi: 10.1128/AAC.03443-14 25001305.

36. Hamblin KA, Armstrong SJ, Barnes KB, Davies C, Wong JP, Blanchard JD, et al. Liposome encapsulation of ciprofloxacin improves protection against highly virulent Francisella tularensis strain Schu S4. Antimicrob Agents Chemother. 2014;58(6):3053–9. Epub 2014/03/19. doi: 10.1128/AAC.02555-13 24637682.

37. Hamblin KA, Armstrong SJ, Barnes KB, Davies C, Laws T, Blanchard JD, et al. Inhaled Liposomal Ciprofloxacin Protects against a Lethal Infection in a Murine Model of Pneumonic Plague. Frontiers in microbiology. 2017;8:91. Epub 2017/02/22. doi: 10.3389/fmicb.2017.00091 28220110.

38. Leighton TJ, Doi RH. The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. J Biol Chem. 1971;246(10):3189–95. Epub 1971/05/25. 4995746.

39. Koransky JR, Allen SD, Dowell VR Jr. Use of ethanol for selective isolation of sporeforming microorganisms. Appl Environ Microbiol. 1978;35(4):762–5. Epub 1978/04/01. 348108.

40. Lyons CR, Lovchik J, Hutt J, Lipscomb MF, Wang E, Heninger S, et al. Murine model of pulmonary anthrax: kinetics of dissemination, histopathology, and mouse strain susceptibility. Infect Immun. 2004;72(8):4801–9. Epub 2004/07/24. doi: 10.1128/IAI.72.8.4801-4809.2004 15271942.

41. Baillie LW, Huwar TB, Moore S, Mellado-Sanchez G, Rodriguez L, Neeson BN, et al. An anthrax subunit vaccine candidate based on protective regions of Bacillus anthracis protective antigen and lethal factor. Vaccine. 2010;28(41):6740–8. Epub 2010/08/10. doi: 10.1016/j.vaccine.2010.07.075 20691267.

42. Loving CL, Kennett M, Lee GM, Grippe VK, Merkel TJ. Murine aerosol challenge model of anthrax. Infect Immun. 2007;75(6):2689–98. Epub 2007/03/14. doi: 10.1128/IAI.01875-06 17353290.

43. Conley J, Yang H, Wilson T, Blasetti K, Di Ninno V, Schnell G, et al. Aerosol delivery of liposome-encapsulated ciprofloxacin: aerosol characterization and efficacy against Francisella tularensis infection in mice. Antimicrob Agents Chemother. 1997;41(6):1288–92. Epub 1997/06/01. 9174185.

44. Wong JP, Yang H, Blasetti KL, Schnell G, Conley J, Schofield LN. Liposome delivery of ciprofloxacin against intracellular Francisella tularensis infection. Journal of controlled release: official journal of the Controlled Release Society. 2003;92(3):265–73. Epub 2003/10/22. doi: 10.1016/s0168-3659(03)00358-4 14568408.

45. Lettieri JT, Rogge MC, Kaiser L, Echols RM, Heller AH. Pharmacokinetic profiles of ciprofloxacin after single intravenous and oral doses. Antimicrob Agents Chemother. 1992;36(5):993–6. Epub 1992/05/01. doi: 10.1128/aac.36.5.993 1510426.

46. Heine HS, Bassett J, Miller L, Hartings JM, Ivins BE, Pitt ML, et al. Determination of antibiotic efficacy against Bacillus anthracis in a mouse aerosol challenge model. Antimicrob Agents Chemother. 2007;51(4):1373–9. Epub 2007/02/14. doi: 10.1128/AAC.01050-06 17296745.


Článek vyšel v časopise

PLOS One


2020 Číslo 1
Nejčtenější tento týden
Nejčtenější v tomto čísle
Kurzy

Zvyšte si kvalifikaci online z pohodlí domova

KOST
Koncepce osteologické péče pro gynekology a praktické lékaře
nový kurz
Autoři: MUDr. František Šenk

Sekvenční léčba schizofrenie
Autoři: MUDr. Jana Hořínková

Hypertenze a hypercholesterolémie – synergický efekt léčby
Autoři: prof. MUDr. Hana Rosolová, DrSc.

Svět praktické medicíny 5/2023 (znalostní test z časopisu)

Imunopatologie? … a co my s tím???
Autoři: doc. MUDr. Helena Lahoda Brodská, Ph.D.

Všechny kurzy
Kurzy Podcasty Doporučená témata Časopisy
Přihlášení
Zapomenuté heslo

Zadejte e-mailovou adresu, se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.

Přihlášení

Nemáte účet?  Registrujte se

#ADS_BOTTOM_SCRIPTS#