Antimicrobial activity of Asteraceae species against bacterial pathogens isolated from postmenopausal women


Autoři: Marcela Oliveira Chiavari-Frederico aff001;  Lidiane Nunes Barbosa aff003;  Isabela Carvalho dos Santos aff003;  Gustavo Ratti da Silva aff003;  Alanna Fernandes de Castro aff001;  Wanessa de Campos Bortolucci aff005;  Lorena Neris Barboza aff001;  Caio Franco de Araújo Almeida Campos aff006;  José Eduardo Gonçalves aff006;  Jacqueline Vergutz Menetrier aff002;  Ezilda Jacomassi aff002;  Zilda Cristiani Gazim aff005;  Samantha Wietzikoski aff001;  Francislaine Aparecida dos Reis Lívero aff002;  Evellyn Claudia Wietzikoski Lovato aff001
Působiště autorů: Laboratory of Preclinical Research of Natural Products, Paranaense University, Umuarama, PR, Brazil aff001;  Medicinal Plants and Phytotherapics in Basic Attention, Paranaense University, Umuarama, PR, Brazil aff002;  Laboratory of Preventive Veterinary Medicine and Public Health, Paranaense University, Umuarama, PR, Brazil aff003;  Animal Sciences with Emphasis on Bioactive Products, Paranaense University, Umuarama, PR, Brazil aff004;  Biotechnology Applied to Agriculture, Chemistry Laboratory of Natural Products, Paranaense University, Umuarama, PR, Brazil aff005;  Clean Technologies, University Center of Maringa, Maringa, PR, Brazil aff006;  Technology and Food Safety and Cesumar Institute of Science, Technology and Innovation – ICETI, University Center of Maringa, Maringa, PR, Brazil aff007
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0227023

Souhrn

Purpose

Investigation of the antibacterial action of aqueous extracts of Bidens sulphurea, Bidens pilosa, and Tanacetum vulgare, species of Asteraceae family that are popularly used for the treatment of genito-urinary infection.

Methods

The minimum inhibitory concentration (MIC) and minimal bacterial concentration (MBC) of the extracts against standard strains of Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli (ATCC25922), and Pseudomonas aeruginosa (ATCC27853) and against bacteria that were isolated from cultures of vaginal secretions and urine from menopausal women with a diagnosis of recurrent urinary tract infections (rUTI) were determined by broth microdilution.

Results

The MIC values of the three extracts against Gram-positive and Gram-negative standard bacterial strains ranged from 7.81 to 125.00 mg ml-1, and the MBC values ranged from 7.81 to 500.00 mg ml-1. However, B. sulphurea was more efficient. In the urine samples, the three extracts inhibited the growth of coagulase-negative Staphylococcus spp., and the B. pilosa was the most active extract against E. coli compared with the other ones. For the vaginal secretion samples, no significant differences in the inhibition of coagulase-positive Staphylococcus spp. and P. mirabilis were found among the extracts. T. vulgare and B. sulphurea were more effective in inhibiting coagulase-negative Staphylococcus spp. compared with B. pilosa. E. coli was more susceptible to the B. sulphurea extract compared with the B. pilosa and T. vulgare extracts.

Conclusion

The present results suggested the potential medicinal use of Asteraceae species, especially B. sulphurea, as therapeutic agents against rUTI-related bacteria.

Klíčová slova:

Antibiotic resistance – Antibiotics – Gram negative bacteria – Gram positive bacteria – Secretion – Staphylococcus – Urine – Proteus mirabilis


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