Pyramiding QTLs controlling tolerance against drought, salinity, and submergence in rice through marker assisted breeding

Autoři: Valarmathi Muthu aff001;  Ragavendran Abbai aff001;  Jagadeeshselvam Nallathambi aff001;  Hifzur Rahman aff001;  Sasikala Ramasamy aff001;  Rohit Kambale aff001;  Thiyagarajan Thulasinathan aff001;  Bharathi Ayyenar aff001;  Raveendran Muthurajan aff001
Působiště autorů: Centre for Plant Molecular Biology and Biotechnology Tamil Nadu Agricultural University, Coimbatore, India aff001
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article


Increases in rice productivity are significantly hampered because of the increase in the occurrence of abiotic stresses, including drought, salinity, and submergence. Developing a rice variety with inherent tolerance against these major abiotic stresses will help achieve a sustained increase in rice production under unfavorable conditions. The present study was conducted to develop abiotic stress-tolerant rice genotypes in the genetic background of the popular rice variety Improved White Ponni (IWP) by introgressing major effect quantitative trait loci (QTLs) conferring tolerance against drought (qDTY1.1, qDTY2.1), salinity (Saltol), and submergence (Sub1) through a marker assisted backcross breeding approach. Genotyping of early generation backcrossed inbred lines (BILs) resulted in the identification of three progenies, 3-11-9-2, 3-11-11-1, and 3-11-11-2, possessing all four target QTLs and maximum recovery of the recurrent parent genome (88.46%). BILs exhibited consistent agronomic and grain quality characters compared to those of IWP and enhanced performance against dehydration, salinity, and submergence stress compared with the recurrent parent IWP. BILs exhibited enhanced tolerance against salinity during germination and increased shoot length, root length, and vigor index compared to those of IWP. All three BILs exhibited reduced symptoms of injury because of salinity (NaCl) and dehydration (PEG) than did IWP. At 12 days of submergence stress, BILs exhibited enhanced survival and greater recovery, whereas IWP failed completely. BILs were found to exhibit on par grain and cooking quality characteristics with their parents. Results of this study clearly demonstrated the effects of the target QTLs in reducing damage caused by drought, salinity, and submergence and lead to the development of a triple stress tolerant version of IWP.

Klíčová slova:

Drought – Drought adaptation – Leaves – Plant resistance to abiotic stress – Quantitative trait loci – Rice – Salinity – Seedlings


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