Discovery of Jogalong virus, a novel hepacivirus identified in a Culex annulirostris (Skuse) mosquito from the Kimberley region of Western Australia


Autoři: Simon H. Williams aff001;  Avram Levy aff003;  Rachel A. Yates aff001;  Nilusha Somaweera aff004;  Peter J. Neville aff004;  Jay Nicholson aff004;  Michael D. A. Lindsay aff004;  John S. Mackenzie aff003;  Komal Jain aff001;  Allison Imrie aff002;  David W. Smith aff002;  W. Ian Lipkin aff001
Působiště autorů: Center for Infection and Immunity, Mailman School of Public Health of Columbia University, New York, New York, United States of America aff001;  Faculty of Health and Medical Sciences, University of Western Australia, Nedlands, Western Australia, Australia aff002;  PathWest Laboratory Medicine WA, Nedlands, Western Australia, Australia aff003;  Environmental Health Directorate, Public and Aboriginal Health Division, Department of Health, Western Australia, Perth, Western Australia, Australia aff004;  Faculty of Health Sciences, Curtin University, Perth, Western Australia, Australia aff005
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0227114

Souhrn

The discovery of hepaciviruses in non-human hosts has accelerated following the advancement of high-throughput sequencing technology. Hepaciviruses have now been described in reptiles, fish, birds, and an extensive array of mammals. Using metagenomic sequencing on pooled samples of field-collected Culex annulirostris mosquitoes, we discovered a divergent hepacivirus-like sequence, named Jogalong virus, from the Kimberley region in northern Western Australia. Using PCR, we screened the same 300 individual mosquitoes and found just a single positive sample (1/300, 0.33%). Phylogenetic analysis of the hepacivirus NS5B protein places Jogalong virus within the genus Hepacivirus but on a distinct and deeply rooted monophyletic branch shared with duck hepacivirus, suggesting a notably different evolutionary history. Vertebrate barcoding PCR targeting two mitochondrial genes, cytochrome c oxidase subunit I and cytochrome b, indicated that the Jogalong virus-positive mosquito had recently fed on the tawny frogmouth (Podargus strigoides), although it is currently unknown whether this bird species contributes to the natural ecology of this virus.

Klíčová slova:

Birds – Blood – Ducks – Hepacivirus – Mosquitoes – Phylogenetic analysis – Polymerase chain reaction – Sequence assembly tools


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2020 Číslo 1