Long term conjugated linoleic acid supplementation modestly improved growth performance but induced testicular tissue apoptosis and reduced sperm quality in male rabbit

Autoři: A. M. Abdelatty aff001;  O. A. M. Badr aff002;  S. A. Mohamed aff002;  M. S. Khattab aff003;  SH. M. Dessouki aff004;  O. A. A. Farid aff005;  A. A. Elolimy aff006;  O. G. Sakr aff004;  M. A. Elhady aff009;  G. Mehesen aff004;  M. Bionaz aff010
Působiště autorů: Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff001;  Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Qalyubia, Egypt aff002;  Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff003;  Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt aff004;  Department of Physiology, National Organization for Drug Control and Research, Giza, Egypt aff005;  Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America aff006;  Arkansas Children’s Nutrition Center, Little Rock, AR, United States of America aff007;  Department of Animal Production, National Research Centre, Giza, Egypt aff008;  Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt aff009;  Department of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR, United States of America aff010
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0226070


Conjugated linoleic acid (CLA) is known for its multiple benefits including improvement of growth, increasing lean mass, and anti-carcinogenic effects. However, when used in long-term supplementations CLA does not improve semen parameters in boar and bull and reduces fertility in Japanese quails. The content of unsaturated fatty acids in dietary lipids plays a significant role in spermatogenesis owning the high proportion of unsaturated fatty acids in plasma membrane of sperms. Whether CLA plays a role in testicular tissue and epididymal fat is still unknown. Therefore, in this study we hypothesize that long-term supplementation of equal proportion of CLA isomer mix (c9,t11-CLA and t10,c12- CLA) in rabbit bucks might alter male reproductive potentials. Twelve V-Line weaned male rabbits were used in 26 weeks trial, rabbits were individually raised and randomly allocated into three dietary groups. Control group (CON) received a basal diet, a group received 0.5% CLA (CLA 0.5%), and a group received 1% CLA (CLA 1%). Rabbits were euthanized at the end of the trial and several parameters were evaluated related to growth, semen quality, and testicular and epididymal tissue histopathology and transcriptome. The long-term supplementation of CLA increased feed intake by 5% and body weight by 2–3%. CLA 1% decreased sperm progressive motility. In testicular tissue L-carnitine and α-tocopherol were decreased by CLA supplementation. In epididymal fat, CLA tended to decrease concentration of polyunsaturated fatty acids, the expression of SCD5 gene was upregulated by CLA 1% and CASP3 gene was upregulated by CLA 0.5%. Transcription of PPARG was downregulated by CLA. Feeding 1% CLA also decreased testicular epithelial thickness. Long-term supplementation of CLA modestly enhanced male rabbit growth, but negatively impacted male reproduction, especially at high dose of CLA.

Klíčová slova:

Body weight – Diet – Fats – Fatty acids – Hematoxylin staining – Rabbits – Semen – Sperm


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