Disparate effects of antibiotic-induced microbiome change and enhanced fitness in Daphnia magna


Autoři: Asa Motiei aff001;  Björn Brindefalk aff002;  Martin Ogonowski aff001;  Rehab El-Shehawy aff001;  Paulina Pastuszek aff002;  Karin Ek aff001;  Birgitta Liewenborg aff001;  Klas Udekwu aff002;  Elena Gorokhova aff001
Působiště autorů: Department of Environmental Science & Analytical Chemistry (ACES), Stockholm University, Stockholm, Sweden aff001;  Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden aff002;  Aquabiota Water Research AB, Stockholm, Sweden aff003;  Swedish University of Agricultural Sciences, Department of Aquatic Resources, Institute of Freshwater Research, Drottningholm, Sweden aff004
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0214833

Souhrn

It is a common view that an organism’s microbiota has a profound influence on host fitness; however, supporting evidence is lacking in many organisms. We manipulated the gut microbiome of Daphnia magna by chronic exposure to different concentrations of the antibiotic Ciprofloxacin (0.01–1 mg L-1), and evaluated whether this affected the animals fitness and antioxidant capacity. In line with our expectations, antibiotic exposure altered the microbiome in a concentration-dependent manner. However, contrary to these expectations, the reduced diversity of gut bacteria was not associated with any fitness detriment. Moreover, the growth-related parameters correlated negatively with microbial diversity; and, in the daphnids exposed to the lowest Ciprofloxacin concentrations, the antioxidant capacity, growth, and fecundity were even higher than in control animals. These findings suggest that Ciprofloxacin exerts direct stimulatory effects on growth and reproduction in the host, while microbiome- mediated effects are of lesser importance. Thus, although microbiome profiling of Daphnia may be a sensitive tool to identify early effects of antibiotic exposure, disentangling direct and microbiome-mediated effects on the host fitness is not straightforward.

Klíčová slova:

Antibiotics – Antioxidants – Bacteria – Daphnia – Fecundity – Gut bacteria – Microbiome – Shannon index


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