Aqueous extract from Mangifera indica Linn. (Anacardiaceae) leaves exerts long-term hypoglycemic effect, increases insulin sensitivity and plasma insulin levels on diabetic Wistar rats

Autoři: Gustavo Roberto Villas Boas aff001;  João Marcos Rodrigues Lemos aff003;  Matheus William de Oliveira aff003;  Rafael Claudino dos Santos aff002;  Ana Paula Stefanello da Silveira aff003;  Flávia Barbieri Bacha aff003;  Caren Naomi Aguero Ito aff003;  Ediane Bortolotte Cornelius aff003;  Fernanda Brioli Lima aff003;  Andrea Marisa Sachilarid Rodrigues aff003;  Nathália Belmal Costa aff003;  Felipe Francisco Bittencourt aff003;  Fernando Freitas de Lima aff002;  Marina Meirelles Paes aff001;  Priscila Gubert aff001;  Silvia Aparecida Oesterreich aff002
Působiště autorů: Research Group on Development of Pharmaceutical Products (P&DProFar), Center for Biological and Health Sciences, Federal University of Western Bahia, Barreiras, Bahia, Brazil aff001;  Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil aff002;  Faculty of Health Sciences, University Center of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil aff003;  Department of Biochemistry, Laboratory of Imunopathology Keizo Asami, Federal University of Pernambuco, Recife, Brazil aff004
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0227105



Diabetes mellitus is one of the most common todays public health problems. According to a survey by the World Health Organization, this metabolic disorder has reached global epidemic proportions, with a worldwide prevalence of 8.5% in the adult population.


The present study aimed to investigate the hypoglycemic effect of aqueous extract of Mangifera indica (EAMI) leaves in streptozotocin-induced diabetic rats.


Sixty male rats were divided into 2 groups: Normoglycemic and Diabetic. Each group was subdivided into negative control, glibenclamide 3 or 10 mg/kg, EAMI 125, 250, 500, and 1000 mg/kg. Intraperitoneal injection of streptozotocin 100 mg/kg was used to DM induction. The hypoglycemic response was assessed acutely after two and four weeks of treatment. After a 6-hour fasting period, the fasting blood glucose of animals was verified, and 2.5 g/kg glucose solution was orally administered. The insulin tolerance test and plasma insulin levels assessment were performed in the morning after fasting of 12 to 14 hours.

Results and conclusion

The chemical analysis of EAMI showed high levels of phenolic compounds. There was no significant difference in fasting blood glucose between normoglycemic and diabetic groups, and that EAMI did not have an acute effect on diabetes. After two and four weeks of treatment, the extract significantly reduced blood glucose levels, exceeding glibenclamide effects. EAMI was effective in maintaining the long-term hypoglycemic effect, as well as, significantly increased the sensitivity of diabetic animals to insulin and the plasma insulin level.

Klíčová slova:

Blood plasma – Blood sugar – Diabetes mellitus – Glucose – Hypoglycemics – Insulin – Leaves – Phenols


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