Dense carbon-nanotube coating scaffolds stimulate osteogenic differentiation of mesenchymal stem cells

Autoři: Hideki Mori aff001;  Yuko Ogura aff002;  Kenta Enomoto aff002;  Masayuki Hara aff001;  Gjertrud Maurstad aff003;  Bjørn Torger Stokke aff003;  Shinichi Kitamura aff002
Působiště autorů: Graduate School of Science, Osaka Prefecture University, Sakai, Osaka, Japan aff001;  Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan aff002;  Biophysics and Medical Technology, Department of Physics, NTNU The Norwegian University of Science and Technology, Trondheim, Norway aff003;  Center for Research and Development of Bioresources, Osaka Prefecture University, Sakai, Japan aff004
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article


Carbon nanotubes (CNTs) have desirable mechanical properties for use as biomaterials in orthopedic and dental area such as bone- and tooth- substitutes. Here, we demonstrate that a glass surface densely coated with single-walled carbon nanotubes (SWNTs) stimulate the osteogenic differentiation of rat bone marrow mesenchymal stem cells (MSCs). MSCs incubated on SWNT- and multi-walled carbon nanotube (MWNT)-coated glass showed high activities of alkaline phosphatase that are markers for early stage osteogenic differentiation. Expression of Bmp2, Runx2, and Alpl of MSCs showed high level in the early stage for MSC incubation on SWNT- and MWNT-coated surfaces, but only the cells on the SWNT-coated glass showed high expression levels of Bglap (Osteocalcin). The cells on the SWNT-coated glass also contained the most calcium, and their calcium deposits had long needle-shaped crystals. SWNT coating at high density could be part of a new scaffold for bone regeneration.

Klíčová slova:

Carbon nanotubes – Cell differentiation – Coatings – Glass – Mesenchymal stem cells – Osteoblast differentiation – Osteoblasts – Laboratory glassware


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