Morphological identification of Amphitetranychus species (Acari: Tetranychidae) with crossbreeding, esterase zymograms and DNA barcode data


Autoři: Tea Arabuli aff001;  Mohamed Waleed Negm aff001;  Tomoko Matsuda aff001;  Yasuki Kitashima aff001;  Tea Abramishvili aff006;  Igor Andrijovych Akimov aff007;  Olga Valentynivna Zhovnerchuk aff007;  Sergei Yakovlevich Popov aff008;  Tetsuo Gotoh aff001
Působiště autorů: Laboratory of Applied Entomology and Zoology, Faculty of Agriculture, Ibaraki University, Ami, Ibaraki, Japan aff001;  Institute of Zoology, Ilia State University, Kakutsa Cholokashvilli Ave, Tbilisi, Georgia aff002;  Institute of Entomology, Agricultural University of Georgia, Kakha Bendukidze Campus, David Aghamashenebeli Alley, Tbilisi, Georgia aff003;  Department of Plant Protection, Faculty of Agriculture, Assiut University, Assiut, Egypt aff004;  Japan Society for the Promotion of Science, Chiyoda, Tokyo, Japan aff005;  Department of Plant Pest Diagnostic, Laboratory of the Ministry of Agriculture, Vasil Godziashvili, Tbilisi, Georgia aff006;  Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine, Vul. B. Khmelnytskogo, Kyiv, Ukraine aff007;  Department of Plant Protection, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya, Moscow, Russia aff008;  Faculty of Economics, Ryutsu Keizai University, Ryugasaki, Ibaraki, Japan aff009
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0221951

Souhrn

The genus Amphitetranychus Oudemans (Tetranychidae) consists of only three species, A. quercivorus (Ehara & Gotoh), A. savenkoae (Reck) and A. viennensis (Zacher). The original description of A. savenkoae was extremely simple and had no drawing of the aedeagus; however, a subsequent study described only the aedeagus. The present study investigated all three species in detail using a combination of morphological traits, crossbreeding experiments, esterase zymograms and the mitochondrial cytochrome c oxidase subunit I (COI) gene. Morphological differences in the peritremes and male aedeagi were observed among the three species. Complete reproductive isolation was confirmed in the reciprocal crosses between the morphologically similar A. savenkoae and A. quercivorus (no female offspring were produced). Esterase zymograms differed interspecifically, but not intraspecifically (among individuals in a given species). All three species formed clearly separate clades with 100% bootstrap values in the COI tree, and A. savenkoae was more closely related to A. quercivorus than to A. viennensis, which corresponded to the morphological similarity of their aedeagi and setal counts on tarsi IV. A key to Amphitetranychus species is provided.

Klíčová slova:

Biology and life sciences – Anatomy – Musculoskeletal system – Body limbs – Legs – Biochemistry – Enzymology – Enzymes – Hydrolases – Esterases – Proteins – Bioenergetics – Energy-producing organelles – Mitochondria – Taxonomy – Organisms – Eukaryota – Animals – Invertebrates – Arthropoda – Mites – Cell biology – Cellular structures and organelles – Plant science – Plant anatomy – Leaves – Medicine and health sciences – Computer and information sciences – Data management – People and places – Geographical locations – Asia – Japan – Research and analysis methods – Database and informatics methods – Biological databases – Bioinformatics – Sequence analysis – Sequence databases


Zdroje

1. Jeppson LR, Keifer HH, Baker EW. Mites injurious to economic plants. University of California Press: Berkeley; 1975.

2. Helle W, Sabelis MW. editors. Spider mites. Their biology, natural enemies, and control. Elsevier: Amsterdam; 1985.

3. Migeon A, Dorkeld F. Spider Mites Web: a comprehensive database for the Tetranychidae; 2019 [cited 2019 January 15]. Available from: http://www.montpellier.inra.fr/CBGP/spmweb

4. Oudemans AC. Acarologische Aanteekeningen CVII. Entomologische Berichten, Amsterdam; 1931; 8: 221–236.

5. Zacher F. Vorläufige Diagnosen einiger neuen Spinnmilbenarten. Berlin, Germany: 1; 1920.

6. Hirst S. Revision of the English species of red spider (Genera Tetranychus and Oligonychus). Proc Zool Soc London; 1920; 4: 49–60.

7. Zacher F. Neue und wenig bekannte Spinnmilben. Z Ang Entomol. 1921; 7: 181–187.

8. Reck GF. Novye vidy tetranihovyh klescej iz Vostocnoj Gruzii. Trudy Instituta Zoologyi Akademyi Nauk Gruzinskoi SSR; 1956; 15: 5–28. (in Russian)

9. Reck GF. A key to the tetranychoid mites. Fauna Trans Caucasia Akademyi Nauk. Gruzinskoi SSR; 1959; 1: 1–152. (in Russian)

10. Wainstein BA. Tetranychoid Mites of Kazakhstan. Publishing House of the Academy of Science of Kazakhistan, Alma-Ata. 1960; 5: 276 pp. (in Russian)

11. Arabuli T. Tetranychoid mites (Acari: Tetranychoidea) fauna of Georgia. Proceedings of Georgian Academy of Science, Biology, Series B; 2008; 6: 86–97.

12. Mitrofanov VI, Strunkova ZI, Livshits IZ. Keys to the tetranychid mites (Tetranychidae, Bryobiidae). Fauna of the USSR and Adjacent Countries. SSR, Dushanbe, Donish; 1987.

13. Zhovnerchuk OV. Tetranychoid mites (Acari, Tetranychoidea) from the Fagales plants in Ukraine. Proceedings of the V International Scientific Conference Readings in memory of prof. II Barabash-Nikiforov, Voronej, Publishing center Voronezh State University, pp. 68–72; 2013.

14. Akimov ІА, Zhovnerchuk ОV. Current status and tendencies in diversity and trophic specialization of Tetranychidae mites (Acari, Tetranychidae) of the steppe zone of Ukraine. Ukrainian Entomol J. 2016; 592: 105–110.

15. Ehara S, Gotoh T. A new Tetranychus closely related to T. viennensis Zacher (Acari: Tetranychidae). Int J Acarol. 1990; 16: 55–58.

16. Khaing TM, Lee JH, Lee WG, Lee KL. A new record of Amphitetranychus quercivorus (Acari: Tetranychidae) in Korea and molecular comparison with A. viennensis. J Asia-Pac Entomol. 2013; 16: 155–160.

17. Ehara S. Some spider mites from Northern Japan. J Fac Sci Hokkaido Univ Zool. 1956; 12: 244–258.

18. Tseng YH. A monograph of the mite family Tetranychidae (Acarina: Tronbidiformes) from Taiwan. Taiwan Museum Special Publication Series; 1990; 9: 1–226.

19. Navajas M, Gutierrez J, Gotoh T. Convergence of molecular and morphological data reveals phylogenetic information on Tetranychus species and allows the restoration of the genus Amphitetranychus (Acari: Tetranychidae). Bull Entomol Res. 1997; 87: 283–288.

20. Navajas M, Gutierrez J, Lagnel J, Fauvel G, Gotoh T. DNA sequences and cross-breeding experiments in the hawthorn spider mite Amphitetranychus viennensis reveal high genetic differentiation between Japanese and French populations. Entomol Exp Appl. 1999; 90: 113–122.

21. Lindquist EE. Anatomy, phylogeny and systematics. External anatomy. In: Helle W, Sabelis MW, editors. Spider mites. Their biology, natural enemies and control. vol. 1A. Elsevier: Amsterdam; 1985. pp. 3–28.

22. Simon C, Frati F, Backenbach A, Crespi B, Liu H, Flook P. Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am. 1994; 87: 651–701.

23. Gotoh T, Araki R, Boubou A, Migeon A, Ferragut F, Navajas M. Evidence of co-specificity between Tetranychus evansi and Tetranychus takafujii (Acari: Prostigmata, Tetranychidae): comments on taxonomic and agricultural aspects. Int J Acarol. 2009; 35: 485–501.

24. Matsuda T, Fukumoto C, Hinomoto N, Gotoh T. DNA-based identification of spider mites: molecular evidence for cryptic species of the genus Tetranychus (Acari: Tetranychidae). J Econ Entomol. 2013; 106: 463–472. doi: 10.1603/ec12328 23448063

25. Matsuda T, Morishita M, Hinomoto N, Gotoh T. Phylogenetic analysis of the spider mite sub-family Tetranychinae (Acari: Tetranychidae) based on the mitochondrial COI gene and the 18S and the 5' end of the 28S rRNA genes indicates that several genera are polyphyletic. PLOS ONE. 2014; 9(10):e108672. doi: 10.1371/journal.pone.0108672 25289639

26. Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016; 33: 1870–1874. doi: 10.1093/molbev/msw054 27004904

27. SPSS. Manual of PASW® Statistics Base 18. Tokyo, Japan: SPSS Japan Inc. (in Japanese); 2009.

28. Sokal RR, Rohlf FJ. Biometry 3rd edition. New York, USA: W. H. Freeman & Company; 1995.

29. Geijskes DC. Beiträge zur Kenntnis der europäischen Spinnmilben (Acari, Tetranychidae) mit besonderer Berücksichtigung der niederländischen Arten. Mededeelingen van de Landbouwhoogeschool, Wageningen; 1939; 42: 1–68.

30. Pritchard AE, Baker EW. A revision of the spider mite family Tetranychidae. Memoir Series 2. Pacific Coast Entomological Society: San Francisco; 1955.

31. Ehara S. Revision of the spider mite family Tetranychidae of Japan (Acari, Prostigmata). Spec Div. 1999; 4: 63–141.

32. Gotoh T, Takayama K. Developmental characteristics, genetic compatibility and esterase zymograms in three strains of the hawthorn spider mite, Tetranychus viennensis Zacher (Acari: Tetranychidae). J Acarol Soc Jpn. 1992; 1: 45–60.

33. Enohara K, Amano H. Simple method for discriminating six common species of red Tetranychus spider mites (Acari: Tetranychidae) in Japan. Jpn J Appl Entomol Zool. 1996; 40: 311–315 (in Japanese).

34. Gotoh T, Kitashima Y, Goka K. Tetranychus mite species identification using esterase and phosphoglucomutase zymograms. Appl Entomol Zool. 2007; 42: 579–585.

35. Klimov PB, Lekveishvili M, Dowling APG, O’Connor BM. Multivariate analysis of morphological variation in two cryptic species of Sancassania (Acari: Acaridae) from Costa Rica. Ann Entomol Soc Am. 2004; 97: 322–345.

36. Famah Sourassou N, Hanna R, Zannou I, Breeuwer JAJ, Moraes G. de, Sabelis MW. Morphological, molecular and cross-breeding analysis of geographic populations of coconut-mite associated predatory mites identified as Neoseiulus baraki: evidence for cryptic species? Exp Appl Acarol. 2012; 57: 15–36. doi: 10.1007/s10493-012-9534-0 22407586


Článek vyšel v časopise

PLOS One


2019 Číslo 9
Nejčtenější tento týden