Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1


Autoři: Ji Hong Kim aff001;  Chae Hwan Cho aff001;  Myung Yi Ryu aff001;  Jong-Gil Kim aff002;  Sei-Jung Lee aff001;  Tae Jung Park aff002;  Jong Pil Park aff001
Působiště autorů: Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan, Republic of Korea aff001;  Department of Chemistry, Institute of Interdisciplinary Convergence Research, Research Institute of Halal Industrialization Technology, Chung-Ang University, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0222144

Souhrn

Dengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed for the detection of dengue virus NS1. A series of amino acid-substituted synthetic peptides was rationally designed, chemically synthesized and covalently immobilized to a gold sensor surface. The sensor performance was monitored via square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Potential affinity peptides specific for NS1 were chosen according to the dynamic current decrease in SWV experiments. Using circular dichroism, the molar ellipticity of peptides (DGV BP1–BP5) was determined, indicating that they had a mostly similar in random coil structure, not totally identical. Using SWV, DGV BP1 was selected as a promising recognition peptide and limit of detection for NS1 was found to be 1.49 μg/mL by the 3-sigma rule. DGV BP1 showed good specificity and stability for NS1, with low signal interference. The validation of the sensor to detect NS1 proteins was confirmed with four dengue virus culture broth (from serotype 1 to 4) as proof-of-concept. The detection performance of our sensor incorporating DGV BP1 peptides showed a statistically significant difference. These results indicate that this strategy can potentially be used to detect the dengue virus antigen, NS1, and to diagnosis dengue fever within a miniaturized portable device in point-of-care testing.

Klíčová slova:

Dengue virus – Electrochemistry – Sensory systems – Synthetic peptides – Gold – Dengue fever – Biosensors – Square waves


Zdroje

1. Antunes P, Watterson D, Parmvi M, Burger R, Boisen A, Young P, et al. (2015) Quantification of NS1 dengue biomarker in serum via optomagnetic nanocluster detection. Sci Rep 5: 16145. doi: 10.1038/srep16145 26536916

2. Pabbaraju K, Wong S, Gill K, Fonseca K, Tipples GA, Tellier R (2016) Simultaneous detection of Zika, Chikungunya and Dengue viruses by a multiplex real-time RT-PCR assay. J Clin Virol 83: 66–71. doi: 10.1016/j.jcv.2016.09.001 27614319

3. Ngo HT, Wang H-N, Fales AM, Nicholson BP, Woods CW, Vo-Dinh T (2014) DNA bioassay-on-chip using SERS detection for dengue diagnosis. Analyst 139: 5655–5659. doi: 10.1039/c4an01077a 25248522

4. Meng F, Badierah RA, Almehdar HA, Redwan EM, Kurgan L, Uversky VN (2015) Unstructural biology of the dengue virus proteins. FEBS J 282: 3368–3394. doi: 10.1111/febs.13349 26096987

5. Lim JM, Kim JH, Ryu MY, Cho CH, Park TJ, Park JP (2018) An electrochemical peptide sensor for detection of dengue fever biomarker NS1. Anal Chim Acta 1026: 109–116. doi: 10.1016/j.aca.2018.04.005 29852986

6. Gutsche I, Coulibaly F, Voss JE, Salmon J, d'Alayer J, Ermonval M, et al. (2011) Secreted dengue virus nonstructural protein NS1 is an atypical barrel-shaped high-density lipoprotein. Proc Natl Acad Sci 108): 8003–8008. doi: 10.1073/pnas.1017338108 21518917

7. Kathiresan E, Paramasivan R, Thenmozhi V, Das A, Dhananjeyan KJ, Sankar SG, et al. (2017) Development and multi-use applications of dengue NS1 monoclonal antibody for early diagnosis. RSC Adv 7: 1927–1931.

8. Darwish NT, Sekaran SD, Alias Y, Khor SM (2018) Immunofluorescence-based biosensor for the determination of dengue virus NS1 in clinical samples. J Pharm Biomed Anal 149: 591–602. doi: 10.1016/j.jpba.2017.11.064 29197806

9. Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M (2002) Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 40: 376–381. doi: 10.1128/JCM.40.2.376-381.2002 11825945

10. Cabral-Castro MJ, Peralta RHS, Cavalcanti MG, Puccioni-Sohler M, Carvalho VL, da Costa Vasconcelos PF, et al. (2016) A Luminex-based single DNA fragment amplification assay as a practical tool for detecting and serotyping dengue virus. J Virol Methods 236: 18–24. doi: 10.1016/j.jviromet.2016.07.003 27393681

11. Hosseini S, Ibrahim F, Djordjevic I, Rothan HA, Yusof R, van der Marel C, et al. (2014) Synthesis and characterization of methacrylic microspheres for biomolecular recognition: ultrasensitive biosensor for dengue virus detection. Eur Polym J 60: 14–21.

12. Parkash O, Yean C, Shueb R (2014) Screen printed carbon electrode based electrochemical immunosensor for the detection of dengue NS1 antigen. Diagnostics 4: 165. doi: 10.3390/diagnostics4040165 26852684

13. Peeling RW, Artsob H, Pelegrino JL, Buchy P, Cardosa MJ, Devi S, et al. (2010) Evaluation of diagnostic tests: dengue. Nat Rev Microbiol 8: S30. doi: 10.1038/nrmicro2459 21548185

14. Wang SM, Sekaran SD (2010) Early diagnosis of dengue infection using a commercial dengue duo rapid test kit for the detection of NS1, IGM, and IGG. Amer J Trop Med Hygiene 83: 690–695.

15. Tricou V, Vu HTT, Quynh NVN, Nguyen CVV, Tran HT, Farrar J, et al. (2010) Comparison of two dengue NS1 rapid tests for sensitivity, specificity and relationship to viraemia and antibody responses. BMC Infect Diseases 10: 142.

16. Wasik D, Mulchandani A, Yates MV (2017) A heparin-functionalized carbon nanotube-based affinity biosensor for dengue virus. Biosens Bioelectron 91: 811–816. doi: 10.1016/j.bios.2017.01.017 28152487

17. Cardosa J, Ooi MH, Tio PH, Perera D, Holmes EC, Bibi K, et al. (2009) Dengue virus serotype 2 from a sylvatic lineage isolated from a patient with dengue hemorrhagic fever. PLOS Negl Trop Diseases 3: e423.

18. Chien L-J, Liao T-L, Shu P-Y, Huang J-H, Gubler DJ, Chang G-JJ (2006) Development of real-time reverse transcriptase PCR assays to detect and serotype dengue viruses. J Clin Microbiol 44: 1295–1304. doi: 10.1128/JCM.44.4.1295-1304.2006 16597854

19. Iswardy E, Tsai T-C, Cheng IF, Ho T-C, Perng GC, Chang H-C (2017) A bead-based immunofluorescence-assay on a microfluidic dielectrophoresis platform for rapid dengue virus detection. Biosens Bioelectron 95: 174–180. doi: 10.1016/j.bios.2017.04.011 28453962

20. Yen C-W, de Puig H, Tam JO, Gomez-Marquez J, Bosch I, Hamad-Schifferli K, et al. (2015) Multicolored silver nanoparticles for multiplexed disease diagnostics: distinguishing dengue, yellow fever, and Ebola viruses. Lab Chip 15: 1638–1641. doi: 10.1039/c5lc00055f 25672590

21. Singhal C, Pundir CS, Narang J (2017) A genosensor for detection of consensus DNA sequence of Dengue virus using ZnO/Pt-Pd nanocomposites. Biosens Bioelectron 97: 75–82. doi: 10.1016/j.bios.2017.05.047 28577500

22. Linares EM, Pannuti CS, Kubota LT, Thalhammer S (2013) Immunospot assay based on fluorescent nanoparticles for Dengue fever detection. Biosens Bioelectron 41: 180–185. doi: 10.1016/j.bios.2012.08.005 22981010

23. Hu D, Fry S, Huang J, Ding X, Qiu L, Pan Y, et al. (2013) Comparison of surface plasmon resonance, resonant waveguide grating biosensing and enzyme linked immunosorbent assay (ELISA) in the evaluation of a dengue virus immunoassay. Biosensors 3: 297. doi: 10.3390/bios3030297 25586260

24. Wong WR, Krupin O, Sekaran SD, Mahamd Adikan FR, Berini P (2014) Serological diagnosis of dengue infection in blood plasma using long-range surface plasmon waveguides. Anal Chem 86: 1735–1743. doi: 10.1021/ac403539k 24410440

25. Kim J-G, Baek SH, Kim S, Kim HI, Lee SW, Phan LMT, et al. (2018) Rapid discriminative detection of dengue viruses via loop mediated isothermal amplification. Talanta 190: 391–396. doi: 10.1016/j.talanta.2018.08.019 30172524

26. Park CR, Park SJ, Lee WG, Hwang BH (2018) Biosensors using hybridization chain reaction—design and signal amplification strategies of hybridization chain reaction. Biotechnol Bioprocess Eng 23: 355–370.

27. Yang JM, Kim KR, Kim CS (2018) Biosensor for rapid and sensitive detection of influenza virus. Biotechnol Bioprocess Eng 23: 371–382.

28. Sinawang P, Rai V, Ionescu RE, Marks RS (2016) Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein. Biosens Bioelectron 77: 400–408. doi: 10.1016/j.bios.2015.09.048 26433352

29. Silva MMS, Dias ACMS, Silva BVM, Gomes-Filho, Kubota LT, Goulart MOF, et al. (2015) Electrochemical detection of dengue virus NS1 protein with a poly(allylamine)/carbon nanotube layered immunoelectrode. J Chem Technol Biotechnol 90: 194–200.

30. Wasik D, Mulchandani A, Yates MV (2018) Point-of-use nanobiosensor for detection of dengue virus NS1 antigen in adult Aedes aegypti: a potential tool for improved dengue surveillance. Anal Chem 90: 679–684. doi: 10.1021/acs.analchem.7b03407 29182305

31. Abdolahad M, Shashaani H, Janmaleki M, Mohajerzadeh S (2014) Silicon nanograss based impedance biosensor for label free detection of rare metastatic cells among primary cancerous colon cells, suitable for more accurate cancer staging. Biosens Bioelectron 59: 151–159. doi: 10.1016/j.bios.2014.02.079 24727600

32. Wu J, Cropek DM, West AC, Banta S (2010) Development of a troponin I biosensor using a peptide obtained through phage display. Anal Chem 82: 8235–8243. doi: 10.1021/ac101657h 20831206

33. Jahanshahi P, Wei Q, Jie Z, Zalnezhad E (2018) Designing a non-invasive surface acoustic resonator for ultra-high sensitive ethanol detection for an on-the-spot health monitoring system. Biotechnol Bioprocess Eng 23: 394–404.

34. Lee D, Hwang J, Seo Y, Gilad AA, Choi J (2018) Optical immunosensors for the efficient detection of target biomolecules. Biotechnol Bioprocess Eng 23: 123–133.

35. Yoon J-Y, Kim D-H, Kim S, Kim D, Jo G, Shin M-S, et al. (2017) Generation of a monoclonal antibody that has reduced binding activity to VX-inactivated butyrylcholinesterase (BuChE) compared to BuChE by phage display. Biotechnol Bioprocess Eng 22: 114–119.

36. Baek SH, Kim MW, Park CY, Choi CS, Kailasa SK, Park JP, et al. (2019) Development of a rapid and sensitive electrochemical biosensor for detection of human norovirus via novel specific binding peptides. Biosens Bioelectron 123: 223–229. doi: 10.1016/j.bios.2018.08.064 30195404

37. Hwang HJ, Ryu MY, Park CY, Ahn J, Park HG, Choi C, et al. (2017) High sensitive and selective electrochemical biosensor: Label-free detection of human norovirus using affinity peptide as molecular binder. Biosens Bioelectron 87: 164–170. doi: 10.1016/j.bios.2016.08.031 27551996

38. Hwang HJ, Ryu MY, Lee GB, Park JP (2016) Selection of high affinity peptides for prediction of colorectal adenoma–to-carcinoma progression. ChemistrySelect 1: 1140–1143.

39. Lim JM, Ryu MY, Cho CH, Kim JH, Yun JW, Park JP (2017) Electrochemical peptide sensor for diagnosing adenoma-carcinoma transition in colon cancer. Biosensor Bioelectron 98: 330–337.

40. Lim JM, Heo NS, Ho SY, Ryu MY, Seo JH, Park TJ, Huh YS, Park JP (2018) Selection of affinity peptides for interference-free detection of cholera toxin. Biosens Bioelectron 99: 289–295. doi: 10.1016/j.bios.2017.07.075 28780344

41. Meirinho SG, Dias LG, Peres AM, Rodrigues LR (2017) Electrochemical aptasensor for human osteopontin detection using a DNA aptamer selected by SELEX. Anal Chim Acta 987: 25–37. doi: 10.1016/j.aca.2017.07.071 28916037

42. Nowinski AK, Sun F, White AD, Keefe AJ, Jiang S (2012) Sequence, structure, and function of peptide self-assembled monolayers. J Am Chem Soc 134: 6000–6005. doi: 10.1021/ja3006868 22401132

43. Desai D, Kumar A, Bose D, Datta M (2018) Ultrasensitive sensor for detection of early stage chronic kidney disease in human. Biosens Bioelectron 105: 90–94. doi: 10.1016/j.bios.2018.01.031 29412950

44. Parkash O, Yean CY, Shueb RH (2014) Screen printed carbon electrode based electrochemical immunosensor for the detection of dengue NS1 antigen. Diagnostics 4: 165. doi: 10.3390/diagnostics4040165 26852684


Článek vyšel v časopise

PLOS One


2019 Číslo 9
Nejčtenější tento týden