Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations
Autoři:
Rola M. Labib aff001; Iriny M. Ayoub aff001; Haidy E. Michel aff002; Mina Mehanny aff003; Verena Kamil aff005; Meryl Hany aff005; Mirette Magdy aff005; Aya Moataz aff005; Boula Maged aff005; Ahmed Mohamed aff005
Působiště autorů:
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
aff001; Department of Pharmcology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
aff002; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
aff003; Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Center for Infection Research (HZI), Saarland University, Saarbrücken, Germany
aff004; Drug Design Program, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
aff005
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0219561
Souhrn
The present study investigates the wound healing potential of three chitosan-based topical preparations loaded with either tea tree essential oil, rosemary essential oil or a mixture of both oils in vivo. Essential oils of M. alternifolia and R. officinalis were analyzed using GC/MS. Essential oil-loaded chitosan topical preparations were formulated. Wound healing potential was evaluated in vivo using an excision wound model in rats. GC/MS analysis of M. alternifolia and R. officinalis essential oils revealed richness in oxygenated monoterpenes, representing 51.06% and 69.61% of the total oil composition, respectively. Topical application of chitosan-based formulation loaded with a mixture of tea tree and rosemary oils resulted in a significant increase in wound contraction percentage compared to either group treated with individual essential oils and the untreated group. Histopathological examination revealed that topical application of tea tree and rosemary oil combination demonstrated complete re-epithelialization associated with activated hair follicles. The high percentage of oxygenated monoterpenes in both essential oils play an important role in the antioxidant and wound healing potential observed herein. Incorporation of tea tree and rosemary essential oils in chitosan-based preparations in appropriate combination could efficiently promote different stages of wound healing.
Klíčová slova:
Biology and life sciences – Biochemistry – Lipids – Oils – Antioxidants – Physiology – Physiological processes – Tissue repair – Wound healing – Nutrition – Diet – Beverages – Tea – Anatomy – Biological tissue – Connective tissue – Granulation tissue – Integumentary system – Hair – Skin – Hair follicles – Medicine and health sciences – Immunology – Immune response – Inflammation – Diagnostic medicine – Signs and symptoms – Pathology and laboratory medicine
Zdroje
1. Budovsky A, Yarmolinsky L, Ben-Shabat S.Effect of medicinal plants on wound healing. Wound. Repair Regen. 2015; 23(2): 171–183. doi: 10.1111/wrr.12274 25703533
2. Kasuya A, Tokura Y.Attempts to accelerate wound healing. J Dermatol. Sci. 2014; 76(3): 169–172. doi: 10.1016/j.jdermsci.2014.11.001 25468357
3. Woollard AC, Tatham KC, Barker S.The influence of essential oils on the process of wound healing: a review of the current evidence. J Wound. Care. 2007; 16(6): 255–257. doi: 10.12968/jowc.2007.16.6.27064 17722522
4. Carson CF, Hammer KA, Riley TV. Melaleuca alternifolia (Tea Tree) oil: a review of antimicrobial and other medicinal properties. Clin Microbiol Rev. 2006; 19(1): 50–62. doi: 10.1128/CMR.19.1.50-62.2006 16418522
5. Drury S. Tea Tree Oil: A Medicine Kit in a Bottle. Random House. 2011.
6. Hammer K. Treatment of acne with tea tree oil (Melaleuca) products: a review of efficacy, tolerability and potential modes of action. International journal of antimicrobial agents. 2015; 45(2): 106–110. doi: 10.1016/j.ijantimicag.2014.10.011 25465857
7. Syed TA, Qureshi ZA, Ali SM, Ahmad S, Ahmad SA. Treatment of toenail onychomycosis with 2% butenafine and 5% Melaleuca alternifolia (tea tree) oil in cream. Tropical Medicine & International Health.1999; 4(4): 284–287.
8. Satchell AC, Saurajen A, Bell C, Barnetson R. Treatment of interdigital tinea pedis with 25% and 50% tea tree oil solution: A randomized, placebo-controlled, blinded study. Australian Journal of Dermatology. 2002; 43(3): 175–178.
9. Yadav E, Kumar S, Mahant S, Khatkar S, Rao R. Tea tree oil: a promising essential oil. Jessent. oil research. 2017; 29(3): 201–213.
10. Rašković A, Milanović I, Pavlović N, Ćebović T, Vukmirović S, Mikov M. Antioxidant activity of rosemary (Rosmarinus officinalis L.) essential oil and its hepatoprotective potential. BMC Complementary and Alternative Medicine. 2014; 14(1): 225.
11. Yu MH, Choi JH, Chae IG, Im HG, Yang SA, More K, Lee IS, Lee J. Suppression of LPS-induced inflammatory activities by Rosmarinus officinalis L. Food Chemistry. 2013; 136(2): 1047–1054. doi: 10.1016/j.foodchem.2012.08.085 23122161
12. Abu-Al-Basal MA.Healing potential of Rosmarinus officinalis L. on full-thickness excision cutaneous wounds in alloxan-induced-diabetic BALB/c mice. J Ethnopharmacol. 2010;131(2): 443–50. doi: 10.1016/j.jep.2010.07.007 20633625
13. de Medeiros Barbosa I, da Costa Medeiros JA, de Oliveira KÁR, Gomes-Neto NJ, Tavares JF, Magnani M, de Souza EL. Efficacy of the combined application of oregano and rosemary essential oils for the control of Escherichia coli, Listeria monocytogenes and Salmonella Enteritidis in leafy vegetables. Food control. 2016; 59: 468–477.
14. Fiume MM, Bergfeld WF, Belsito DV, Hill RA, Klaassen CD, Liebler DC, et al. Safety Assessment of Rosmarinus officinalis (Rosemary)-Derived Ingredients as Used in Cosmetics. International Journal of Toxicology. 2018 37(3-suppl): 12S–50S.
15. Saporito F, Sandri G, Bonferoni MC, Rossi S, Boselli C, Icaro CA, et al. Essential oil-loaded lipid nanoparticles for wound healing. Int. J Nanomedicine. 2018; 13: 175–186. doi: 10.2147/IJN.S152529 29343956
16. Patrulea V, Ostafe V, Borchard G, Jordan O.Chitosan as a starting material for wound healing applications. Eur. J Pharm. Biopharm. 2015; 97(Pt B): 417–426. doi: 10.1016/j.ejpb.2015.08.004 26614560
17. Pelissari FM, Grossmann MV, Yamashita F, Pineda EA. Antimicrobial, mechanical, and barrier properties of cassava starch-chitosan films incorporated with oregano essential oil. J Agric Food Chem. 2009; 57(16): 7499–504. doi: 10.1021/jf9002363 19627142
18. Raafat D, Sahl HG.Chitosan and its antimicrobial potential-a critical literature survey. Microb. Biotechnol. 2009; 2(2): 186–201. doi: 10.1111/j.1751-7915.2008.00080.x 21261913
19. Delaquis PJ, Stanich K, Girard B, Mazza G. Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int. J Food Microbiol. 2002; 74(1–2): 101–109. 11929164
20. Amin M, abdel-Raheem I. Accelerated wound healing and anti-inflammatory effects of physically cross linked polyvinyl alcohol-chitosan hydrogel containing honey bee venom in diabetic rats. Arch. Pharm. Res. 2014; 37(8): 1016–1031. doi: 10.1007/s12272-013-0308-y 24293065
21. Adams RP. Identification of essential oil components by gas chromatography/mass spectorscopy: Allured Publishing Corporation.2007.
22. Elkady WM, Ayoub IM.Chemical profiling and antiproliferative effect of essential oils of two Araucaria species cultivated in Egypt. Industrial Crops and Products. 2018;118:188–195.
23. Labib RM, Youssef FS, Ashour ML, Abdel Daim MM, Ross SA. Chemical composition of Pinus roxburghii bark volatile oil and validation of its anti-Inflammatory activity using molecular modelling and bleomycin-induced inflammation in Albino mice. Molecules. 2017; 22(9): 1384.
24. Wang L, Liu F, Jiang Y, Chai Z, Li P, Cheng Y, et al. Synergistic antimicrobial activities of natural essential oils with chitosan films. J Agric. Food Chem. 2011; 59(23): 12411–12419. doi: 10.1021/jf203165k 22034912
25. Ponrasu T, Suguna L.Efficacy of Annona squamosa on wound healing in streptozotocin-induced diabetic rats. Int Wound J. 2012; 9(6): 613–23. doi: 10.1111/j.1742-481X.2011.00924.x 22233431
26. Kant V, Gopal A, Pathak NN, Kumar P, Tandan SK, Kumar D. Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats. International immunopharmacology. 2014; 20(2): 322–330. doi: 10.1016/j.intimp.2014.03.009 24675438
27. Sardari K, Kakhki EG, Mohri M.Evaluation of wound contraction and epithelializationafter subcutaneous administration of Theranekron in cows. Comp Clin Pathol. 2007; 16: 197–200.
28. Ohkawa H, Ohishi N, Yagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry. 1979; 95(2): 351–358. doi: 10.1016/0003-2697(79)90738-3 36810
29. Kei S. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica chimica acta. 1978; 90(1): 37–43.
30. Beutler E, Duron O, Kelly M.Colorimetric method for determination of glutathione reductase concentration. Journal of Laboratory and Clinical Medicine. 1963; 61: 882. 13967893
31. Saha K, Mukherjee PK, Das J, Pal M, Saha BP. Wound healing activity of Leucas lavandulaefolia Rees. Journal of Ethnopharmacology. 1997; 56(2): 139–144. 9174975
32. Shi C, Zhu Y, Ran X, Wang M, Su Y, Cheng T. Therapeutic potential of chitosan and its derivatives in regenerative medicine. J Surg Res. 2006;133(2): 185–92. doi: 10.1016/j.jss.2005.12.013 16458923
33. Kweon DK, Song SB, Park YY. Preparation of water-soluble chitosan/heparin complex and its application as wound healing accelerator. Biomaterials. 2003; 24(9): 1595–601. 12559819
34. Tripathi S, Mehrotra G, Dutta P. Physicochemical and bioactivity of cross-linked chitosan–PVA film for food packaging applications. International Journal of Biological Macromolecules. 2009; 45(4): 372–376. doi: 10.1016/j.ijbiomac.2009.07.006 19643129
35. Caner C, Vergano P, Wiles J.Chitosan film mechanical and permeation properties as affected by acid, plasticizer, and storage. Journal of food science. 1998; 63(6): 1049–1053.
36. Pazyar N, Yaghoobi R, Bagherani N, Kazerouni A. A review of applications of tea tree oil in dermatology. International Journal of Dermatology. 2013;52(7): 784–790. doi: 10.1111/j.1365-4632.2012.05654.x 22998411
37. Farag RS, Shalaby AS, El-Baroty GA, Ibrahim NA, Ali MA, Hassan EM. Chemical and biological evaluation of the essential oils of different Melaleuca species. Phytotherapy Research. 2004; 18(1): 30–35. doi: 10.1002/ptr.1348 14750197
38. Chin KB, Cordell B. The effect of tea tree oil (Melaleuca alternifolia) on wound healing using a dressing model. J Altern Complement Med. 2013; 19(12): 942–5. doi: 10.1089/acm.2012.0787 23848210
39. Brand C, Ferrante A, Prager RH, Riley TV, Carson CF, Finlay-Jones JJ. et al. The water-soluble components of the essential oil of Melaleuca alternifolia (tea tree oil) suppress the production of superoxide by human monocytes, but not neutrophils, activated in vitro. Inflam Res. 2001; 50(4): 213–9.
40. Silva CJ, Barbosa LCA, Maltha CRA, Pinheiro AL, Ismail FMD. Comparative study of the essential oils of seven Melaleuca (Myrtaceae) species grown in Brazil. Flavour and Fragrance Journal. 2007; 22(6): 474–478.
41. Takaki I, Bersani-Amado LE, Vendruscolo A, Sartoretto SM, Diniz SP, Bersani-Amado CA, et al. Anti-inflammatory and antinociceptive effects of Rosmarinus officinalis L. essential oil in experimental animal models. J Med Food. 2008; 11(4): 741–6. doi: 10.1089/jmf.2007.0524 19053868
Článek vyšel v časopise
PLOS One
2019 Číslo 9
- Proč jsou nemocnice nepřítelem spánku? A jak to změnit?
- Dlouhodobá ketodieta může poškozovat naše orgány
- „Jednohubky“ z klinického výzkumu – 2024/42
- Metamizol jako analgetikum první volby: kdy, pro koho, jak a proč?
- MUDr. Jana Horáková: Remise již dosahujeme u více než 80 % pacientů s myastenií