Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach


Autoři: Mathilde Scheifler aff001;  Magdalena Ruiz-Rodríguez aff001;  Sophie Sanchez-Brosseau aff001;  Elodie Magnanou aff001;  Marcelino T. Suzuki aff002;  Nyree West aff003;  Sébastien Duperron aff004;  Yves Desdevises aff001
Působiště autorů: Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Observatoire Océanologique, Banyuls/Mer, France aff001;  Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes, LBBM Observatoire Océanologique, Banyuls/Mer, France aff002;  Sorbonne Université, CNRS, Observatoire Océanologique de Banyuls, Banyuls/Mer, France aff003;  CNRS, Muséum National d’Histoire Naturelle, Molécules de Communication et Adaptation des Micro-organismes, UMR7245 MCAM, Muséum National d’Histoire Naturelle, Paris, France aff004
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0221475

Souhrn

Next‐generation sequencing methods are increasingly used to identify eukaryotic, unicellular and multicellular symbiont communities within hosts. In this study, we analyzed the non-specific reads obtained during a metabarcoding survey of the bacterial communities associated to three different tissues collected from 13 wild Mediterranean teleost fish species. In total, 30 eukaryotic genera were identified as putative parasites of teleosts, associated to skin mucus, gills mucus and intestine: 2 ascomycetes, 4 arthropods, 2 cnidarians, 7 nematodes, 10 platyhelminthes, 4 apicomplexans, 1 ciliate as well as one order in dinoflagellates (Syndiniales). These results highlighted that (1) the metabarcoding approach was able to uncover a large spectrum of symbiotic organisms associated to the fish species studied, (2) symbionts not yet identified in several teleost species were putatively present, (3) the parasitic diversity differed markedly across host species and (4) in most cases, the distribution of known parasitic genera within tissues is in accordance with the literature. The current work illustrates the large insights that can be gained by making maximum use of data from a metabarcoding approach.

Klíčová slova:

Biology and life sciences – Anatomy – Body fluids – Mucus – Animal anatomy – Aquatic respiratory anatomy – Gills – Respiratory system – Digestive system – Gastrointestinal tract – Physiology – Organisms – Eukaryota – Animals – Invertebrates – Flatworms – Nematoda – Zoology – Parasitology – Intestinal parasites – Medicine and health sciences – Parasitic diseases – Parasitic intestinal diseases


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