Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro
Autoři:
Prashant Kumar Modi aff001; Ashwini Prabhu aff001; Yashodhar P. Bhandary aff001; Sudheer Shenoy P. aff001; Aparna Hegde aff001; Sindhu Priya ES aff001; Renjith P. Johnson aff001; Shankar Prasad Das aff001; Sahil Vazirally aff002; Punchappady-Devasya Rekha aff001
Působiště autorů:
Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, Karnataka, India
aff001; Global Calcium Pvt. Ltd., Bangalore, Karnataka, India
aff002
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0222240
Souhrn
Calcium is the key macromineral having a role in skeletal structure and function, muscle contraction, and neurotransmission. Bone remodeling is maintained through a constant balance between calcium resorption and deposition. Calcium deficiency is resolved through calcium supplementation, and among the supplements, water-soluble organic molecules attracted great pharmaceutical interest. Calcium glucoheptonate is a highly water-soluble organic calcium salt having clinical use; however, detailed investigations on its biological effects are limited. We assessed the effects of calcium glucoheptonate on cell viability and proliferation of osteoblast-like MG-63 cells. Calcium uptake and mineralization were evaluated using Alizarin red staining of osteoblast-like MG-63 cells treated with calcium glucoheptonate. Expression of osteogenic markers were monitored by western blotting, immunofluorescence, and qRT-PCR assays. Increased proliferation and calcium uptake were observed in the MG-63 cells treated with calcium glucoheptonate. The treatment also increased the expression of osteopontin and osteogenic genes such as collagen-1, secreted protein acidic and cysteine rich (SPARC), and osteocalcin. Calcium glucoheptonate treatment did not exert any cytotoxicity on colorectal and renal epithelial cells, indicating the safety of the treatment. This is the first report with evidence for its beneficial effect for pharmaceutical use in addressing calcium deficiency conditions.
Klíčová slova:
Biology and life sciences – Biochemistry – Hormones – Peptide hormones – Osteocalcin – Enzymology – Enzymes – Phosphatases – Proteins – Cell biology – Cellular types – Animal cells – Epithelial cells – Anatomy – Biological tissue – Epithelium – Physiology – Physiological processes – Bone remodeling – Ossification – Research and analysis methods – Specimen preparation and treatment – Staining – Cell staining – Group-specific staining – Alizarin staining – Biological cultures – Cell lines – 293T cells – Bioassays and physiological analysis – Biochemical analysis – Colorimetric assays – MTT assay – Enzyme assays – Medicine and health sciences
Zdroje
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PLOS One
2019 Číslo 9
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