Identification of candidate flowering and sex genes in white Guinea yam (D. rotundata Poir.) by SuperSAGE transcriptome profiling


Autoři: Gezahegn Girma aff001;  Satoshi Natsume aff003;  Anna Vittoria Carluccio aff001;  Hiroki Takagi aff003;  Hideo Matsumura aff003;  Aiko Uemura aff003;  Satoru Muranaka aff004;  Hiroko Takagi aff004;  Livia Stavolone aff001;  Melaku Gedil aff001;  Charles Spillane aff002;  Ryohei Terauchi aff003;  Muluneh Tamiru aff003
Působiště autorů: Bioscience center, International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, Nigeria aff001;  Plant and AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway, Ireland aff002;  Department of Genomics and Breeding, Iwate Biotechnology Research Center (IBRC), Kitakami, Iwate, Japan aff003;  Japan International Research Center for Agricultural Sciences (JIRCAS), Ohwashi, Tsukuba, Japan aff004
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0216912

Souhrn

Dioecy (distinct male and female individuals) and scarce to non-flowering are common features of cultivated yam (Dioscorea spp.). However, the molecular mechanisms underlying flowering and sex determination in Dioscorea are largely unknown. We conducted SuperSAGE transcriptome profiling of male, female and monoecious individuals to identify flowering and sex-related genes in white Guinea yam (D. rotundata), generating 20,236 unique tags. Of these, 13,901 were represented by a minimum of 10 tags. A total 88 tags were significantly differentially expressed in male, female and monoecious plants, of which 18 corresponded to genes previously implicated in flower development and sex determination in multiple plant species. We validated the SuperSAGE data with quantitative real-time PCR (qRT-PCR)-based analysis of the expression of three candidate genes.

We further investigated the flowering patterns of 1938 D. rotundata accessions representing diverse geographical origins over two consecutive years. Over 85% of accessions were either male or non-flowering, less than 15% were female, while monoecious plants were rare. Intensity of flowering varied between male and female plants, with the former flowering more abundantly than the latter. Candidate genes identified in this study can be targeted for further validation and to induce regular flowering in poor to non-flowering cultivars. Findings of the study provide important inputs for further studies aiming to overcome the challenge of flowering in yams and to improve efficiency of yam breeding.

Klíčová slova:

Flowering plants – Flowers – Gene expression – Gene regulation – Sequence databases – Sex determination – Expressed sequence tags analysis – Floral development


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