Hypervirulent Klebsiella pneumoniae serotype K1 clinical isolates form robust biofilms at the air-liquid interface


Autoři: Meritxell Cubero aff001;  Sara Marti aff001;  Mª Ángeles Domínguez aff001;  Aida González-Díaz aff001;  Dàmaris Berbel aff001;  Carmen Ardanuy aff001
Působiště autorů: Department of Microbiology, Hospital Universitari de Bellvitge, Instituto de Investigación Biomédica de Bellvitge (IDIBELL), Barcelona, Spain aff001;  Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, Spain aff002;  Spanish Network for Research in Infectious Diseases (REIPI), Barcelona, Spain aff003;  Departamento de Patología y terapéutica experimental, Universitat de Barcelona, Barcelona, Spain aff004
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222628

Souhrn

The prevalence of a new hypervirulent and hypermucoviscous K. pneumoniae phenotype (Hmv) is increasing worldwide, mainly linked to serotypes K1 and K2. Since capsular thickness can directly affect the capability to form biofilms, we aimed to evaluate the association between the Hmv phenotype with adhesion and biofilm formation in a collection of clinical K. pneumoniae isolates.

We selected 38 Hmv clinical isolates [15 serotype K1; 9 serotype K2; 3 non-K1/K2 (rmpA+); 11 non-K1/K2 (rmpA-)] and 7 non-Hmv clinical isolates. The Hmv phenotype was assessed through the mucoviscosity test. Serum resistance was determined by bacterial viability tests in pooled human serum. Adhesion was evaluated with the Biofilm Ring Test®, and biofilm formation was identified by crystal violet staining (Solid-Liquid, SLI-biofilm) or visual examination (Air-Liquid, ALI-biofilm).

This study linked for the first time the formation of robust ALI-biofilm plugs by K. pneumoniae to the capsular serotype K1, a group of hypervirulent strains which are generally highly susceptible to the antimicrobial agents. Among all the studied isolates, the capsular serotype K1 presented lower initial adhesion despite having the adhesins mrkD and fimH but higher ALI-biofilm formation than isolates with other capsular serotypes (K2 or non-K1/K2). This structure might confer increased resistance to a group of hypervirulent K. pneumoniae serotype K1.

Klíčová slova:

Biology and life sciences – Organisms – Bacteria – Klebsiella – Microbiology – Medical microbiology – Microbial pathogens – Bacterial pathogens – Bacteriology – Bacterial biofilms – Bacterial physiology – Adhesins – Biofilms – Microbial physiology – Cell biology – Cellular structures and organelles – Pili and fimbriae – Medicine and health sciences – Pathology and laboratory medicine – Pathogens – Virulence factors – Pathogen motility – Research and analysis methods – Specimen preparation and treatment – Staining – Crystal violet staining


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2019 Číslo 9

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