Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice


Autoři: Zhongquan Cai aff001;  Peilong Jia aff002;  Jiaqiang Zhang aff003;  Ping Gan aff001;  Qi Shao aff001;  Gang Jin aff004;  Liping Wang aff004;  Jian Jin aff001;  Jiangyi Yang aff001;  Jijing Luo aff001
Působiště autorů: College of Life Science and Technology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, China aff001;  Institute for New Rural Development, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, China aff002;  Research and Development Centre of Flower, Zhejiang Academy of Agricultural Sciences, Hangzhou, China aff003;  Guangxi Subtropical Crops Research Institute, Nanning, China aff004
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223228

Souhrn

Chloroplast plays an important role in the plant life cycle. However, the details of its development remain elusive in rice. In this study, we report the fine-mapping of a novel rice gene wpb1 (white panicle branch 1), which affects chloroplast biogenesis, from a tropical japonica variety that results in an albino panicle branches at and after the heading stage. The wpb1 variety was crossed with Nipponbare to generate the F2 and BC1F2 populations. Green and white panicle branch phenotypes with a 3:1 segregation ratio was observed in the F2 population. Bulked segregant analysis (BSA) based on whole genome resequencing was conducted to determine the wpb1 locus. A candidate interval spanning from 11.35 to 23.79M (physical position) on chromosome 1 was identified. The results of BSA analysis were verified by a 40K rice SNP-array using the BC1F2 population. A large-scale F2 population was used to pinpoint wpb1, and the locus was further narrowed down to a 95-kb interval. Furthermore, our results showed that the expression levels of the majority of the genes involved in Chl biosynthesis, photosynthesis and chloroplast development were remarkably affected in wpb1 variety and in F2 plants with a white panicle branch phenotype. In line with the results mentioned above, anatomical structural examination and chlorophyll (Chl) content measurement suggested that wpb1 might play an important role in the regulation of chloroplast development. Further cloning and functional characterization of the wpb1 gene will shed light on the molecular mechanism underlying chloroplast development in rice.

Klíčová slova:

Biosynthesis – Genetic loci – Chlorophyll – Chloroplasts – Leaves – Photosynthesis – Rice – Panicles


Zdroje

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