Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation

Autoři: Mahesh Attimarad aff001;  Venugopla Katarigatta Narayanswamy aff001;  Bandar Essa Aldhubaib aff001;  Nagaraja SreeHarsha aff001;  Anroop Balachandran Nair aff001
Působiště autorů: Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa, KSA aff001;  Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa aff002
Vyšlo v časopise: PLoS ONE 14(9)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222526


Recently, the United States Food and Drug Administration approved a new oral dosage preparation of amlodipine besylate (AML) and celecoxib (CEL) for the management of hypertension and osteoarthritis. However, no simultaneous estimation procedures for these two analytes have been described. Hence, two simple, accurate, and precise ultraviolet spectroscopic procedures that manipulated the ratio spectra were established for concurrent quantification of AML and CEL using ethanol as a solvent. The first method involves determining the peak-to-trough amplitude difference of the ratio spectra of AML and CEL. The second method involves determining the peak amplitude of the ratio first derivative (Δλ 4 nm) spectra of AML and CEL at 334.2 nm and 254.2 nm, correspondingly. Both methods showed linearity in the range of 1–6 μg mL-1 for AML and 5–40 μg mL-1 for CEL with an excellent correlation coefficient (<0.999). The proposed procedures were validated by following the International Conference on Harmonization guidelines for accuracy, precision, selectivity, recovery, and stability studies. It is evident from the low %RSD and %RE that both analytical procedures were found to be accurate and precise, respectively. The percent recovery of AML and CEL from the formulation was found to be 99.79% and 99.34% using the ratio-difference method and 100.13% and 99.70% using the ratio first-derivative method, with a low percent relative standard deviation. Further, the proposed techniques permit concurrent quantification of AML and CEL in different concentration ratios without interference from each other; hence, these techniques can be adopted for regular quality-control studies.

Klíčová slova:

Physical sciences – Chemistry – Chemical compounds – Organic compounds – Alcohols – Ethanol – Organic chemistry – Physics – Electromagnetic radiation – Absorption spectra – Light – Ultraviolet radiation – Materials science – Materials – Mixtures – Medicine and health sciences – Rheumatology – Arthritis – Osteoarthritis – Vascular medicine – Blood pressure – Hypertension – Pharmacology – Drugs – Analgesics – NSAIDs – Antihypertensive drugs – Pain management


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