Roles of differential expression of miR-543-5p in GH regulation in rat anterior pituitary cells and GH3 cells
Autoři:
Ze-Wen Yu aff001; Wei Gao aff001; Xin-Yao Feng aff001; Jin-Yu Zhang aff001; Hai-Xiang Guo aff001; Chang-Jiang Wang aff001; Jian Chen aff001; Jin-Ping Hu aff001; Wen-Zhi Ren aff001; Bao Yuan aff001
Působiště autorů:
Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China
aff001
Vyšlo v časopise:
PLoS ONE 14(9)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0222340
Souhrn
Growth hormone (GH) is an important hormone released by the pituitary gland that plays a key role in the growth and development of organisms. In our study, TargetScan analysis and the dual luciferase reporter assays were used to predict and screen for miRNAs that might act on the rat Gh1 gene, and we identified miR-543-5p. Then, the GH3 cell line and the primary rat pituitary cells were transfected with miRNA mimic, inhibitor, and siRNA. We detected the Gh1 gene expression and the GH secretion by real-time PCR and ELISAs, respectively, to verify the regulatory effect of miR-543-5p on GH secretion. The results showed that miR-543-5p can inhibit Gh1 mRNA expression and reduce GH secretion. MiR-543-5p inhibitor upregulated Gh1 mRNA expression and increased GH secretion compared with the negative control. In summary, miR-543-5p downregulates Gh1 expression, resulting in a decrease in GH synthesis and secretion, which demonstrates the important role of miRNAs in regulating GH and animal growth and development.
Klíčová slova:
Biology and life sciences – Anatomy – Endocrine system – Pituitary gland – Nervous system – Neuroscience – Biochemistry – Nucleic acids – RNA – Non-coding RNA – Natural antisense transcripts – MicroRNAs – Hormones – Peptide hormones – Growth hormone – Genetics – Gene expression – Gene regulation – Molecular biology – Molecular biology techniques – Transfection – DNA construction – Plasmid construction – Physiology – Physiological processes – Secretion – Cell biology – Cell processes – Cell death – Apoptosis – Medicine and health sciences – Neuroanatomy – Research and analysis methods
Zdroje
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PLOS One
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