Principles of Carbon Catabolite Repression in the Rice Blast Fungus: Tps1, Nmr1-3, and a MATE–Family Pump Regulate Glucose Metabolism during Infection
Understanding the genetic pathways that regulate how pathogenic fungi respond to their environment is paramount to developing effective mitigation strategies against disease. Carbon catabolite repression (CCR) is a global regulatory mechanism found in a wide range of microbial organisms that ensures the preferential utilization of glucose over less favourable carbon sources, but little is known about the components of CCR in filamentous fungi. Here we report three new mediators of CCR in the devastating rice blast fungus Magnaporthe oryzae: the sugar sensor Tps1, the Nmr1-3 inhibitor proteins, and the multidrug and toxin extrusion (MATE)–family pump, Mdt1. Using simple plate tests coupled with transcriptional analysis, we show that Tps1, in response to glucose-6-phosphate sensing, triggers CCR via the inactivation of Nmr1-3. In addition, by dissecting the CCR pathway using Agrobacterium tumefaciens-mediated mutagenesis, we also show that Mdt1 is an additional and previously unknown regulator of glucose metabolism. Mdt1 regulates glucose assimilation downstream of Tps1 and is necessary for nutrient utilization, sporulation, and pathogenicity. This is the first functional characterization of a MATE–family protein in filamentous fungi and the first description of a MATE protein in genetic regulation or plant pathogenicity. Perturbing CCR in Δtps1 and MDT1 disruption strains thus results in physiological defects that impact pathogenesis, possibly through the early expression of cell wall–degrading enzymes. Taken together, the importance of discovering three new regulators of carbon metabolism lies in understanding how M. oryzae and other pathogenic fungi respond to nutrient availability and control development during infection.
Published in the journal:
. PLoS Genet 8(5): e32767. doi:10.1371/journal.pgen.1002673
Category:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1002673
Summary
Understanding the genetic pathways that regulate how pathogenic fungi respond to their environment is paramount to developing effective mitigation strategies against disease. Carbon catabolite repression (CCR) is a global regulatory mechanism found in a wide range of microbial organisms that ensures the preferential utilization of glucose over less favourable carbon sources, but little is known about the components of CCR in filamentous fungi. Here we report three new mediators of CCR in the devastating rice blast fungus Magnaporthe oryzae: the sugar sensor Tps1, the Nmr1-3 inhibitor proteins, and the multidrug and toxin extrusion (MATE)–family pump, Mdt1. Using simple plate tests coupled with transcriptional analysis, we show that Tps1, in response to glucose-6-phosphate sensing, triggers CCR via the inactivation of Nmr1-3. In addition, by dissecting the CCR pathway using Agrobacterium tumefaciens-mediated mutagenesis, we also show that Mdt1 is an additional and previously unknown regulator of glucose metabolism. Mdt1 regulates glucose assimilation downstream of Tps1 and is necessary for nutrient utilization, sporulation, and pathogenicity. This is the first functional characterization of a MATE–family protein in filamentous fungi and the first description of a MATE protein in genetic regulation or plant pathogenicity. Perturbing CCR in Δtps1 and MDT1 disruption strains thus results in physiological defects that impact pathogenesis, possibly through the early expression of cell wall–degrading enzymes. Taken together, the importance of discovering three new regulators of carbon metabolism lies in understanding how M. oryzae and other pathogenic fungi respond to nutrient availability and control development during infection.
Introduction
Fungi cause recalcitrant diseases of humans, animals and plants. In order to survive in environments with limited and variable resources, they have developed elegant and efficient genetic regulatory systems to enable them to respond rapidly to fluctuating nutritional conditions, but little is known about the components of these metabolic control pathways in multicellular fungal pathogens. Carbon and nitrogen metabolic regulation has, however, been extensively studied in model filamentous fungi such as the bread mold Neurospora crassa [1] and the soil saprophyte Aspergillus nidulans [2]–[8]. A. nidulans uses pathway specific gene induction to metabolize a wide range of carbon and nitrogen compounds, but this voracity is tempered by two global regulatory systems that ensure the preferential utilization of a few favoured carbon and nitrogen sources. The positive-acting GATA family transcription factor AreA functions in global nitrogen metabolite repression (NMR) to allow utilization of the most preferred nitrogen sources ammonium (NH4+) and L-glutamine (Figure 1A; reviewed in [7] and [8]). In the presence of NH4+or L-glutamine, the inhibitor protein NmrA [9] interacts with AreA to prevent nitrogen catabolic gene expression, but in the presence of less-preferred nitrogen sources such as nitrate (NO3−), NmrA dissociates from AreA, allowing it to activate the expression of more than 100 genes involved in alternative nitrogen source usage [7]. Carbon catabolite repression (CCR) on the other hand, operates via the negatively-acting zinc finger repressor CreA [4], [6], [10], [11] to ensure glucose is utilized preferentially by preventing the expression of genes required for the metabolism of less preferred carbon sources (Figure 1B).
Interestingly, both CCR and NMR regulatory systems converge on genes required for metabolizing a few key compounds that can be used as both carbon and nitrogen sources. For example, A. nidulans utilizes proline as both a carbon and nitrogen source [2], [3], [12], [13]. Dual CCR/NMR control of proline utilization ensures proline can be used as a nitrogen source in the presence of a repressing carbon source, and can be used as a carbon source in the presence of a repressing nitrogen source. Moreover, strains carrying areA loss-of-function mutations (areA−) are unable to utilize proline as a source of nitrogen if a repressing carbon source (e.g. glucose) is present, but grow on proline in the presence of non-repressing carbon sources [2], [3]. Thus AreA is only required for the expression of proline structural genes in the presence of an active CreA protein. Loss of growth of areA− strains on glucose+proline media has been used as a selection to generate revertants of areA−, restored for growth on this media, that result from mutations in CreA and the inactivation of CCR [3].
Like other fungal pathogens, the filamentous fungus Magnaporthe oryzae, cause of the devastating rice blast disease [14], [15], also faces challenges of nutrient limitation and variability but in a significantly different environment to that of A. nidulans. Rice blast disease is a grave threat to global food security [16] and results in 10–30% crop loss annually [17], although in some regions destruction of rice can reach 100%. The life cycle of M. oryzae begins when a three-celled conidium lands on the surface of the leaf and germinates [15]. In a nutrient-free and hydrophobic environment (ie. the leaf surface), the germtube swells and forms the dome-shaped infectious cell called the appressorium. Enormous turgor in the appressorium, formed from the accumulation of glycerol, acts on a thin penetration peg emerging from the base of the cell, forcing it through the surface of the leaf. However, this “brute-force” entry mechanism belies the fact that once within the host cell, the fungus spreads undetected from cell to cell in a biotrophic growth phase, extracting nutrients from the host in a manner that does not immediately kill the plant cell [18], [19]. Only after 72 hrs does M. oryzae enter its necrotic phase, forming characteristic lesions on the surface of the leaf from which aerial hyphae release spores to continue the infection process. During the infection cycle, global regulatory systems in M. oryzae must cope temporally with acquiring nutrients by stealth during biotrophy and by absorption during necrotrophy; and must respond spatially to the fluctuations in nutrient quality and quantity encountered throughout the host leaf. Moreover, plate tests show M. oryzae can grow on a wide range of carbon and nitrogen sources likely controlled by NMR and CCR ([20], [21]; Quispe and Wilson, unpublished data).
Although an AreA homologue, Nut1, has been characterized in M. oryzae and is not required for virulence [22], [23], no global regulators of carbon metabolism have been characterized in this fungus. In addition, little is known about CCR in other fungal pathogens, although overexpressing the CREA homologue in the plant pathogen Alternaria citri results in severe symptoms of black rot in citrus fruit [24]; CCR has been shown to be involved in isocitrate lyase and cell wall degrading enzyme production in the tomato pathogen Fusarium oxysporum [25]; and the absence of either hexokinase or glucokinase protein in the human pathogen Aspergillus fumigatus results in loss of CCR and the induction of isocitrate lyase activity in the presence of glucose [26]. Recently, trehalose-6-phosphate synthase (Tps1) has emerged as a glucose-6-phosphate (G6P) sensor that, inter alia, integrates carbon and nitrogen metabolism to regulate infection by M. oryzae [21], [23]. Tps1 controls infection-related gene expression via a novel NADPH-dependent genetic switch. In response to G6P, Tps1 activates glucose-6-phosphate dehydrogenase, leading to the elevated production of the reduced dinucleotide NADPH from NADP and G6P. As NADPH levels increase at the expense of NADP, three M. oryzae homologues of the NmrA inhibitor protein- Nmr1, Nmr2 and Nmr3 - become inactivated, resulting in the activation of at least three GATA factors (including Nut1) and the expression of genes required for pathogenicity (Figure S1).
We undertook this study to determine whether G6P sensing by Tps1 in filamentous fungi regulates carbon metabolism via CCR, to identify what proteins constitute CCR, and to understand how CCR impacts pathogenicity - processes currently unknown in M. oryzae and little understood in other fungi [10], [11]. Here we show for the first time in filamentous fungi that the G6P sensor for triggering CCR is Tps1. We show in M. oryzae how Tps1 regulation of CCR involves Nmr1-3, and how the modulation of CCR by the Nmr1-3 inhibitor proteins occurs independently of Nut1 - thus revealing a hitherto unrecognized role for Nmr-like proteins in carbon regulation. Δnut1 strains, like areA− strains, are unable to grow on proline in the presence of glucose. To identify additional components of CCR and to characterize their role in pathogenicity, we used Agrobacterium tumefaciens- mediated mutagenesis to target CCR by selecting for Δnut1 strains restored in their ability to grow on media containing glucose and proline. In this manner we identified a MATE-family efflux pump [27], Mdt1, as an additional regulator of CCR. Characterization of mutants disrupted in the MDT1 gene showed they were misregulated for carbon metabolism even in the presence of glucose. They were also severely attenuated in sporulation and, although they could form appressoria and were not sensitive to reactive oxygen species (ROS), they were unable to cause disease. Therefore, we demonstrate Mdt1 is essential for nutrient adaptability and pathogenicity in M. oryzae. In toto, this work describes three new classes of global carbon metabolic regulators in filamentous fungi; it is the first study to characterize a MATE-family efflux pump in filamentous and plant pathogenic fungi; and is the first study to assign a regulatory function to a MATE protein in any organism.
Results/Discussion
Genes for metabolizing compounds that are both carbon and nitrogen sources are subject to CCR and NMR in M. oryzae
This study began with an interest in understanding how the metabolism of compounds having the potential to be both carbon and nitrogen sources are regulated in M. oryzae. Our initial investigations found that Δnut1 strains generated by Wilson et al. in a previous study [23] could not utilize three such compounds - aminoisobutyric acid, proline and glucosamine – in the presence of glucose compared to the wild type Guy11 strain. The inability of Δnut1 strains to grow on proline as a nitrogen source contradicts an earlier study by Froeliger and Carpenter, where deletion of NUT1 was shown to allow growth on proline [22]. We therefore independently generated new Δnut1 strains (Figure 2A) and verified that they also cannot grow on proline, in addition to aminoisobutyric acid and glucosamine, in the presence of glucose. This suggests the metabolism of proline, glucosamine and aminoisobutyric acid requires an active Nut1 protein for utilization as nitrogen sources when glucose is present (Figure 2A).
Other than an inability to use proline as a nitrogen source, in all other aspects, our Δnut1 strains have the same phenotype as that reported by Froeliger and Carpenter [22]. This includes an inability to grow on defined minimal media containing nitrate (NO3−) or nitrite as sole nitrogen sources (Figure S2A); good growth on ammonium (NH4+), glutamate and alanine as sole nitrogen sources (Figure S2A); and small lesion sizes on host leaf [23]. We cannot explain this discrepancy, but in light of the analyses that follow, we conclude deleting NUT1 abolishes proline utilization in the presence of glucose.
We next determined that the wild type strain, Guy11, could not utilize aminoisobutyric acid as a carbon source (Figure S2B). This compound is therefore not both a carbon and nitrogen source for M. oryzae, and was excluded from further analysis. Focusing on glucosamine and proline, we found that although unable to use these compounds as sole nitrogen sources in the presence of glucose, Δnut1 strains, like Guy11, utilized these compounds as carbon sources in the absence of glucose - both in the presence and absence of a repressing nitrogen source (NH4+) (Figure 2B). This suggests these compounds do not require an active Nut1 when metabolized as a carbon source and are therefore under CCR control. In addition, Δnut1 strains were restored for growth on proline as a nitrogen source in the presence of the derepressing carbon sources xylose and sorbitol (Figure 2C), confirming the metabolism of these compounds is subject to both CCR and nitrogen metabolite repression. We conclude that an active Nut1 protein is required for using these dual compounds as nitrogen sources in the presence of glucose (ie. when CCR is active), but is not required in the absence of glucose or in the presence of derepressing carbon sources (ie. when CCR is inactive) (Figure 2D).
G6P sensing by Tps1 is required to activate CCR
The above results suggested that CCR plays an active regulatory role in M. oryzae carbon metabolism. We continued our characterization of carbon metabolism in the rice blast fungus by determining what role, if any, Tps1 might play in carbon regulation. Tps1 is a G6P sensor that integrates carbon and nitrogen metabolism and is essential for pathogenicity. In response to G6P, Tps1 modulates NADPH levels to inactivate the Nmr1-3 inhibitor proteins and activate transcription factors including Nut1 [23]. Thus, Δtps1 mutants cannot grow on nitrate as nitrogen source because the Nmr1-3 inhibitor proteins constitutively inactivate Nut1 in this strain [21], [23], [28]. Δtps1 strains are also affected in glycogen metabolism [21], suggesting Tps1 might regulate carbon metabolism. To determine how extensive Tps1-dependent carbon regulation might be, we generated a Δtps1 Δnut1 double mutant and showed that, unlike the Δnut1 single mutant, it can utilize proline and glucosamine as nitrogen sources in the presence of glucose (Figure 3A). This suggests CCR, at least for proline and glucosamine metabolism, is Tps1-dependent.
In addition to compounds that are both carbon and nitrogen sources, might Tps1-dependent CCR also regulate the metabolism of compounds that are carbon sources only? In the presence of glucose, CCR is known to inhibit the expression of genes encoding alcohol dehydrogenases that convert alcohols into acetyl-coA. Allyl alcohol is used as an assay for carbon derepression because it is converted by alcohol dehydrogenase to the very toxic compound acrylaldehyde. Wild type M. oryzae strains are resistant to allyl alcohol when grown on repressing carbon sources (i.e. glucose) but inactivation of CCR by derepressing carbon sources renders M. oryzae sensitive to allyl alcohol [20]. Mutations that inactivate CCR should also result in carbon derepression and sensitivity to allyl alcohol in the presence of glucose. In our study, Δtps1 mutant strains were grown on a glucose-rich minimal media containing 55 mM glucose (ie. 1% glucose) with 10 mM NH4+ as sole carbon and nitrogen source, respectively, with or without 100 mM allyl alcohol (AA). Figure 3B and Figure S3A show that, compared to Guy11, ally alcohol was extremely toxic to Δtps1 strains at this concentration, suggesting Δtps1 strains were strongly derepressed for alcohol metabolism in the presence of glucose. This indicates Tps1 controls CCR to regulate, in addition to proline and glucosamine, broad aspects of carbon metabolism in response to glucose.
We next asked whether regulation of CCR by Tps1 occurs via G6P sensing. G6P and UDP-glucose are native substrates for Tps1. Previous work showed Tps1 proteins carrying the amino acid substitutions R22G or Y99V in the G6P binding pocket were abolished for trehalose-6-phosphate production but could still sense G6P and were pathogenic, thus demonstrating a sugar signaling role for Tps1 independent of its biosynthetic function [21]. We found that compared to Δtps1 strains, strains carrying the constructs Δtps1::R22G (Figure 3B and Figure S3A) and Δtps1::Y99V (Figure S3A) - encoding the R22G and Y99V substitutions in Tps1, respectively - were insensitive to 100 mM AA in the presence of glucose and, unlike the Δtps1 parental strains, were not inactivated for CCR. Thus, G6P sensing by Tps1 is required for CCR.
In Saccharomyces cerevisiae, phosphorylation of glucose and fructose by the hexokinase protein Hxk2p results in CCR [29]. In addition, Hxk2p regulates CCR independently of hexose phosphorylation because mutant Hxk2p proteins with reduced catalytic activity still demonstrate some glucose repression, suggesting Hxk2p might induce CCR via a non-metabolic process likely requiring nuclear localization [30]. Magnaporthe oryzae carries genes encoding two putative hexokinases (HXK1 and HXK2) and one glucokinase (GLK1). Δhxk1 [21] and Δglk1 [31] gene deletion strains are fully pathogenic, but the role of these genes in CCR has not been examined. To determine if Magnaporthe hexose kinase proteins have a non-metabolic role in CCR upstream of Tps1 in the G6P signaling pathway, we deleted GLK1, HXK1 and the previously uncharacterized HXK2 gene from the Guy11 genome by homologues gene replacement [23] and tested the resulting deletion strains for loss of CCR. Figure S3B shows that neither hexose kinase deletion strain demonstrated susceptibility to 100 mM allyl alcohol in the presence of 55 mM glucose, suggesting CCR is still operating in these deletion strains. Thus, unlike yeast but similar to A. nidulans [11], loss of the hexokinase or glucokinase proteins in Magnaporthe does not affect CCR. However, multiple hexose kinase deletion mutants would be expected to be inactive for CCR in the presence of glucose by virtue of their inability to form G6P, the trigger for CCR. The generation and analysis of multiple hexose kinase gene deletion strains is a future goal of our research.
Taken together, these results suggest G6P sensing by Tps1 is the key step in the regulation of CCR in Magnaporthe (Figure 3C), and is the first report of how G6P triggers CCR in filamentous fungi.
Transcriptional studies, plate growth tests, and proteomic analysis reveal Tps1 regulates glucose metabolism and suppresses alternative carbon source utilization
To understand how Tps1-dependent CCR might regulate carbon metabolism, we used quantitative real time PCR (qPCR) to analyze the expression of genes required for glucose metabolism and alternative carbon source utilization in Guy11, compared to Δtps1 strains, following growth on minimal media containing glucose and NH4+. Nitrogen-repressing media was chosen to eliminate a role for Nut1 in the expression of these genes (see below), but similar fold changes were also seen when the strains were grown on NO3− minimal media (Figure S4). Strains were grown in complete media (CM) for 48 hr before switching to minimal media containing 55 mM glucose with 10 mM NH4+ or 10 mM NO3− as sole nitrogen sources for 16 hr (following [23]). CM is used as the initial growth condition in Magnaporthe switch experiments because when fresh CM is added at 24 hr, it allows strong mycelial growth of Magnaporthe strains without resulting in the rapid melanization of mycelia observed for growth in minimal media. Similarly, mycelia was switched to minimal media for 16 hr to allow maximum gene induction while avoiding the melanization of mycelia that occurs after this time.
By sequence homology to known glucose transporters in yeast, we studied the expression of genes encoding two putative high affinity glucose transporters (GHT2 and RGT2), and one putative low affinity glucose transporter (HXT1) (Figure 4A; Table S1). We also studied the expression of hexose kinase genes likely involved in the first step of glucose metabolism: HXK1, HXK2 and GLK1 (Figure 4B, Table S1). Figure 4A and Figure 4B show that genes for importing and metabolizing glucose are reduced in expression in Δtps1 strains compared to Guy11 during growth on minimal media containing glucose.
The differences in gene expression of glucose transport and metabolism genes in Guy11 or Δtps1 strains were similar regardless of nitrogen source (Figure 4 and Figure S4). One notable exception was GHT2 that appeared to be elevated in Δtps1 strains during growth on NO3− media (Figure S4A) compared to Guy11. Because Δtps1 strains are unable to utilize nitrate, we considered that GHT2 might be expressed in response to nitrogen starvation. To test this, we studied the expression of GHT2 in the mycelia of Guy11 strains grown in NH4+ minimal medium with 55 mM glucose, or in 55 mM glucose minimal media lacking a nitrogen source. Figure S4C shows GHT2 is elevated in Guy11 under nitrogen starvation conditions. Thus, a real lack of a metabolizable nitrogen source (in the case of Guy11 on nitrogen starvation media) or a perceived lack of nitrogen source (in the case of Δtps1 strains on nitrate media) induces GHT2 expresssion, suggesting multiple nutritional signals converge on GHT2. Identifying what these signals might be warrants further analysis in the future.
We also examined the expression of four genes in Guy11 and Δtps1 strains necessary for alternative carbon source utilization following growth on 55 mM glucose and 10 mM NH4+minimal media: PRN3 encoding a putative L-Δ1-pyrroline-5-carboxylate dehydrogenase likely required for proline utilization; GNI1 encoding a putative glucosamine-6-phosphate isomerase/deaminase required for glucosamine metabolism; XYR1 encoding a putative xylose reductase involved in xylose metabolism; and ADH1 encoding a putative alcohol dehydrogenase (Figure 4C and 4D; Table S1). In contrast to glucose importing and metabolizing genes, the expression of genes for utilizing some alternative carbon sources (proline, glucosamine and alcohol but not xylose) are significantly elevated in Δtps1 during growth on glucose (Student's t-test p≤0.05).
The expression of PRN3, GNI1 and XYR1 following growth on nitrate media is shown in Figure S4D. The expression of ADH1 following growth on nitrate media is shown in Figure S4E and is strongly up-regulated in Δtps1 strains.
The expression of a proline-metabolizing gene in Δtps1 in the absence of inducer might arise from internal proline carried over from the nutrient rich CM starter culture. To determine if this is the case, we repeated the mycelial switch experiment of Guy11 and Δtps1 but following 48 hr growth in CM, each strain was transferred to a starvation minimal media lacking both a source of glucose and nitrogen for 12 hr before switching into minimal media with 55 mM glucose and 10 mM NO3− for 16 hr. The rationale is that internal sources of proline should be metabolized during growth under starvation conditions and would not be available to induce proline gene expression during growth in minimal media with a carbon and nitrogen source. Nonetheless, even when including a starvation shake condition, expression of PRN3 was still significantly elevated in Δtps1 strains compared to Guy11 (Student's t-test p≤0.01; Figure S4F), suggesting derepression of at least one proline utilizing gene can occur in Δtps1 strains in the absence of an inducer.
Together with Figure 3B, we conclude that Tps1-mediated CCR, via G6P sensing, is required for the glucose-mediated induction of glucose utilization genes and the repression of genes required for metabolizing alternative carbon sources (Figure 4D).
Next, we considered how loss of CCR in Δtps1 strains affects fungal physiology. Figure 3C and the transcriptional results shown in Figure 4 and Figure S4 indicated Δtps1 strains should be impaired in glucose metabolism due to the inactivation of CCR in the presence of glucose and the resulting abherrant affect on glucose metabolizing gene expression. Altered glucose metabolism in Δtps1 strains compared to Guy11 is supported by two lines of evidence in Figure 3A. First, Figure 3A shows that Δtps1 and Δtps1 Δnut1 strains were reduced for growth on minimal media with 10 mM glucose and 10 mM NH4+ compared to the parental strains (Figure 3A). This is in contrast to previous reports that demonstrated strong growth of Δtps1 on ammonium minimal media [21], [23]. However, previous studies used 1% (ie 55 mM) glucose as carbon source, with nitrate or ammonium as nitrogen source, and Figure 5A shows that Δtps1 strains grew better on ammonium minimal media when high (55 mM) glucose concentrations were used compared to lower (10 mM) levels of glucose. Thus, Δtps1 strains grow poorly on low concentrations of glucose compared to Guy11. It should be noted that Δtps1 strains were not improved for growth on nitrate-media under any glucose conditons tested (up to 10% glucose, not shown), consistent with the hypothesis that Tps1 is required for integrating G6P availability, G6PDH activity and NADPH production during growth on nitrate [21].
A second piece of evidence for glucose metabolic defects of Δtps1 strains comes from the analysis of growth on proline and glucosamine containing minimal media. Figure 3A shows that growth of Δtps1 strains on 10 mM glucose+10 mM proline and 10 mM glucose+10 mM glucosamine minimal media was much weaker than in Guy11, but stronger than growth of Δtps1 strains on 10 mM glucose+10 mM NH4+. This suggested proline and glucosamine might be used as alternative but poorer sources of carbon for Δtps1 strains even in the presence of glucose. To test this, we looked at the growth of Δtps1 on proline and glucosamine as sole carbon and nitrogen sources. Figure 5B shows that compared to growth on 10 mM glucose+10 mM NH4+ media, Δtps1 strains grew stronger on media containing proline or glucosamine as a sole nitrogen source, a sole carbon source, or as both a carbon and nitrogen source. Taken together, deletion of Tps1 results in poor growth on glucose media compared to Guy11, which is partially remediated by alternative, less-preferred carbon sources such as proline and glucosamine.
We next sought to determine whether Δtps1 strains were impaired in glucose metabolism due to defects in sugar uptake and phosphorylation or because they were unable to assimilate phosphorylated glucose. The sugar transport and hexose kinase expression data presented in Figure 4A and 4B suggested that reduced uptake and phosphorylation of glucose by Δtps1 strains might result in low internal G6P levels and the observed loss of CCR. Indeed, a class of carbon derepressed mutants of A. nidulans were found to result from defective glucose uptake [3]. However, several lines of evidence suggest Δtps1 strains are not reduced for glucose uptake and phosphorylation during growth on glucose-rich (55 mM) minimal media. Firstly, Wilson et al. [21] demonstrated that although G6PDH activity was reduced in Δtps1 strains during growth on nitrate compared to Guy11, hexokinase activity in Δtps1 strains was not affected, suggesting different mechanisms for hexokinase transcriptional and post-translational control that warrant further investigation in the future. Secondly, G6P levels are significantly elevated, not depleted, in the mycelia of Δtps1 strains under both NO3− and NH4+ nitrogen regimes [21] suggesting G6P assimilation –via the pentose phoshate pathway - but not G6P production was impaired. Thirdly, although Δtps1 strains grow with reduced hyphal mass on minimal media with 10 mM glucose+10 mM NH4+ compared to Guy11 (Figure 5A), radial growth was not affected, again suggesting glucose assimilation but not uptake is impaired. Indeed, growth of glucose uptake mutants would be significantly inhibited on low glucose media, but the radial growth of Δtps1 strains on low glucose concentrations (0.2% to 0.05% glucose final concentration) was comparable to that of Guy11 on the same media (Figure 6A). This suggested that Δtps1 strains do not grow significantly different to Guy11 on carbon-limiting (ie glucose-derepressing) media, as would be expected if CCR was constitutively inactivated in Δtps1 strains. Finally, the carbon derepressed mutants of A. nidulans that were found to result from defective glucose uptake [3] were also resistant to both the toxic glucose analogue 2-deoxyglucose (2-DOG), which requires uptake and phosphorylation by hexokinase activity for toxicity, and the toxic sugar sorbose [32] during growth under carbon derepressing conditions. When grown on carbon derepressing minimal media comprising 55 mM xylose and 10 mM NH4+ as sole carbon and nitrogen sources, we observed, however, that disruption of Δtps1 did not confer resistance to these toxic analogues (Figure 6B). Taken together, these four lines of evidence indicated uptake and phosphorylation of glucose was not greatly impaired in Δtps1 strains during growth under the conditions tested. This conclusion is consistent with work in A. nidulans that showed CCR inactivation and constitutive carbon derepression in a creAd mutant strain did not impair glucose uptake [3].
We next asked whether impaired growth of Δtps1 strains on glucose media was due to defects in G6P assimilation into the Δtps1 metabolome, such as suggested by the observed G6P accumulation in Δtps1 strains. Glucose assimilating defects could result from the misregulation of CCR in these strains, where genes for metabolizing alternative carbon sources are expressed in the presence of glucose. To determine what affect CCR misregulation might have on glucose metabolism in the cell, we undertook a comparative proteomics study of Δtps1 and Guy11 mycelial samples (Table S2) to identify at least some of the metabolic processes altered in Δtps1. It should be noted that in this proteomics study, absence of a protein from a sample indicates its level of abundance did not reach the threshold of detection by the current LC/MS/MS set-up used and does not necessarily imply it was not present at all. In support our transcriptional data, proteomic analysis of Δtps1 and Guy11 mycelial samples grown in glucose-minimal media showed a putative hexose transporter, MGG_08617 (highlighted in Table S2), was more abundant in Guy11 samples compared to Δtps1 samples and is consistent with the role for Tps1 in regulating glucose uptake and metabolism. In addition, malate dehydrogenase (MGG_09872) was detected in Δtps1 samples but not the Guy11 proteome (highlighted in Table S2). MGG_09872 was predicted by PSORTII to be localized to the cytoplasm (60.9% probability it is localized to the cytoplasm and 8% it is localized to the mitochondrion), indicating it could be involved in the conversion of malate into oxaloacetate during gluconeogenesis. On the other hand, the enzyme enolase (MGG_10607, involved in glycolysis and gluconeogenesis) and 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (MGG_00901) were not detected in Δtps1 samples, but were identified in Guy11 samples, following growth on glucose-containing minimal media (highlighted in Table S2). On the basis of the protein abundance data, some enzymes of gluconeogenesis and glycolysis could be misregulated in Δtps1 strains in the presence of glucose compared to Guy11.
Using the proteomics data as a clue, we sought to determine if Δtps1 strains were impaired for glucose assimilation due to the misregulation of genes associated with gluconeogenesis or glycolysis. We studied the expression of PFK1, encoding phosphofructokinase and considered the most important control element in the glycolytic pathway due to the irreversible phosphorylation of fructose-6-phosphate to give fructose-1,6-bisphosphate; and FBP1 encoding fructose-1,6-bisphosphatase I that performs the reverse reaction to PFK1 in gluconeogenesis by dephosphorylating fructose-1,6-bisphosphate to give fructose-6-phosphate. Figure 7A shows that PFK1 gene expression was elevated in Guy11 strains compared to Δtps1 strains on glucose- minimal media. Conversely, FBP1 was expressed most highly in Δtps1 strains on glucose-minimal media. These results are consistant with a previous study which showed phosphofructokinase activity was decreased, and fructose-1,6-bisphosphate activity was increased, in an Aspergillus strain carrying an extreme creAdmutation, compared to wild type, during growth on glucose [33]. We also looked at the expression of a second important gluconeogenic gene, ICL1, encoding isocitrate lyase. Isocitrate lyase is necessary for the cleavage of isocitrate to succinate and glyoxylate in the glyoxylate cycle and is required for synthesizing glucose via gluconeogenesis from acetyl-CoA. Isocitrate lyase has also been shown to be subject to CCR control in the tomato pathogen Fusarium oxysporum [25]. Figure 7A shows ICL1 gene expression was significantly elevated in Δtps1 strains during growth on glucose compared to Guy11. Therefore, consistent with other CCR mutants, Δtps1 strains are upregulated for the expression of genes for alternative carbon source assimilation and down-regulated, relative to Guy11, for the expression of a central gene of glycolysis, indicating poor growth of Δtps1 strains on glucose could result from impaired glucose assimilation. It should be noted that PFK1 is still expressed in Δtps1 strains, thus allowing some growth on glucose.
The proteomic data in Table S2 also revealed additional genes likely controlled by Tps1 via CCR. Genes encoding cell wall degrading enzymes (CWDEs) have previously been shown to be glucose-repressed and elevated in expression under glucose-derepressing conditions in M. oryzae, although the genes involved in regulating CWDE gene expression in response to carbon source was not previously known [34]. We identified proteins corresponding to putative CWDEs that were more abundantly present in Δtps1 samples than Guy11 samples following growth on glucose-containing minimal media (highlighted in Table S2). These included glucan 1,3-beta-glucosidase (MGG_00263, 26-fold more abundant in Δtps1 samples than Guy11 samples); a putative cutinase G-box binding protein; chitinase 18-11; feruloyl esterase B; ß-glucosidase 1 and exoglucanase 1 (MGG_00501, MGG_06594, MGG_05529, MGG_09272, and MGG_10712 respectively, detected in Δtps1 samples but not detected in Guy11 samples); and D-galacturonic acid reductase (MGG_07463, elevated in abundance in Δtps1 samples compared to Guy11). These observations suggested the expression of CWDE-encoding genes were perturbed in Δtps1 strains and is consistent with a role for Tps1 in repressing the expression of genes required for metabolizing alternative carbon sources, such as cell wall polysaccharides, in the presence of glucose. To confirm this, we first analyzed the in planta expression of the genes encoding ß-glucosidase 1, feruloyl esterase B and exoglucanase (termed CWDE1, CWDE2 and CWDE3, respectively) by isolating RNA from infected leaves at 24 hpi (the time of appressorium penetration), 40 hpi (before necrotic lesions had developed) and 66 hpi (when lesions had formed). Figure 7B shows how each gene is highly expressed during the latter stages of infection. Next, we looked at the expression of these genes in Δtps1 and Guy11 strains following growth in glucose-media. Figure 7C shows that at least feruloyl esterase B and exoglucanase- encoding genes are derepressed in Δtps1 strains compared to Guy11, suggesting they are subjected to Tps1-dependent CCR in the presence of glucose and are misregulated in Δtps1 strains.
Taken together, the plate growth, transcriptional and proteomic data describe an essential role for Tps1 in controlling CCR and allowing the fungus to respond correctly to glucose availability.
Nmr1-3 inhibitor proteins regulate carbon metabolism downstream of Tps1 and independently of Nut1
A previous study showed that in response to G6P sensing, Tps1 alleviates Nmr1-3 protein inhibition via modulation of NADPH levels resulting, inter alia, in nitrogen derepression [23]. Yeast two-hybrid studies demonstrated Nmr1-3 physically interacted with Asd4, an essential regulator of appresorium formation; Nmr2 interacted with the white collar-2 homologue Pas1; and Nmr1 and Nmr3 interacted with Nut1. Interestingly, deletion of all three NMR orthologues was required for full derepression of Nut1 activity under repressing conditions, implying that although not detected in Nut1 binding studies, Nmr2 did have a role in regulating Nut1 activity. In addition, deletion of any one NMR gene in the Δtps1 background partially restored fungal virulence to Δtps1 strains, albeit with reduced lesion sizes compared to Guy11 (shown for Δtps1 Δnmr1 leaf infection in Figure S5). Thus Δtps1 strains have constitutively active Nmr inhibitor proteins, and deleting NMR genes in the Δtps1 background results in activation of Tps1-dependent gene expression and partial suppression of the Δtps1 phenotype [23]. Although Nmr proteins have only previously been described in the literature as mediators of nitrogen metabolism (reviewed in [8]), we sought to establish if Tps1-dependent CCR occurred via Nmr1-3 inhibition in order to shed more light on the role(s) and interaction(s) of Nmr1-3 during infection. We first compared the susceptibility of Δtps1, Δtps1 Δnmr1, Δtps1 Δnmr2 and Δtps1 Δnmr3 strains to 100 mM AA in glucose minimal media under nitrogen repressing conditions. Δnut1 strains were included to determine if the global nitrogen regulator had any influence on AA metabolism. Figure 8A shows that Δtps1 strains were susceptible to 100 mM AA in minimal media containing 55 mM glucose and 10 mM NH4+, whereas the Δtps1 Δnmr1-3 double mutant strains, like Guy11 and Δnut1 strains, were resistant to 100 mM AA and thus restored for CCR. Because Δnut1 and Δtps1 strains do not grow on plates of nitrate-media, we also looked at the expression of ADH1 in these strains after growth on CM followed by a switch to nitrate minimal media. Figure 8B shows ADH1 gene expression was reduced almost 25-fold in Δtps1 Δnmr1-3 double mutant strains compared to the Δtps1 parental strain and confirms CCR is restored to Δtps1 Δnmr1-3 double mutant strains relative to Δtps1. Figure 8A and 8B together show that this modulation of CCR by the Nmr inhibitor proteins occurs irrespective of nitrogen source or an active Nut1 protein.
To further explore a role for the Nmr inhibitor proteins in carbon metabolism and CCR, we next looked at the expression of the Tps1-dependent hexose kinase genes, HXK1 (Figure 8C), HXK2 (Figure S6A) and GLK1 (Figure S6B) in Δtps1, the Δtps1 Δnmr1-3 double mutant strains, and Δnut1 compared to Guy11 following growth on minimal media with nitrate. Nitrate was chosen to determine if expression of these genes requires an active Nut1. Hexose kinase gene expression was shown to be elevated in Δtps1 Δnmr1-3 double mutant strains compared to the Δtps1 single mutant strains (Figure 8C and Figure S6A and S6B). Similarly, expression of the putative hexose transporter gene HXT1 (Figure 8C) was elevated in Δtps1 Δnmr1-3 double mutant strains compared to Δtps1 and in all cases expression was not affected in Δnut1 strains compared to Guy11. In addition, the expression of G6PDH was shown previously to be reduced in Δtps1 strains compared to Guy11 but was restored to wild type levels of expression in the Δtps1 Δnmr1-3 double mutant strains [23], and Figure S6C shows G6PDH gene expression is also independent of Δnut1. Finally, the expression of GNI1, subjected to Tps1-dependent CCR, was partially repressed in Δtps1 Δnmr1-3 double mutant strains compared to Δtps1. Thus, glucose-utilizing genes are expressed, and alternative carbon source utilization is repressed, in Δtps1 Δnmr1-3 double mutant strains compared to Δtps1 strains, in the presence of glucose, while Nut1 is shown to have no role in CCR. Consequently, this is the first description of a role for an NmrA-family protein in regulating both carbon and nitrogen metabolism in a filamentous fungus.
Together, this data suggests the model in Figure 8D, whereby carbon metabolism is regulated by the Nmr1-3 inhibitor proteins independently of Nut1 and in response to G6P sensing by Tps1. Under glucose-repressing conditions, modulation of NADPH levels by Tps1 would inactivate the Nmr inhibitor proteins and result in CCR. Under carbon derepressing conditions, the Nmr inhibitor proteins would be active and suppress CCR. Consistent with this model, we found that Δtps1 Δnmr1 (but not Δtps1 Δnmr2 or Δtps1 Δnmr3) were able to grow on 100 mM AA in the presence of the derepressing carbon source xylose (Figure 8E), suggesting CCR was at least partially active under derepressing conditions in this strain.
Nmr1-3 controls Nut1 in response to glucose
With regards to nitrogen metabolism, the model in Figure 8D also predicts that Nmr1-3 should control Nut1 in response to glucose availability and is consistent with our observations that loss of G6P sensing in Δtps1 strains locks Nut1 in its inactive form regardless of nitrogen source [21], [23]. Additional evidence for the model proposed in Figure 8D comes from studying the activity of Nut1-dependent processes under different nutritional conditions. Nut1 is required to express NIA1, encoding nitrate reductase (NR), under nitrogen derepressing conditions. Nitrate reductase activity was detected in M. oryzae mycelial samples grown under NR inducing conditions (glucose and NO3− minimal media, Figure 9A) but was absent following growth under nitrogen repressing conditions, ie glucose and NH4+ [23]. NR activity was also not detected in NR induction media lacking a source of carbon (−C+NO3−, Figure 9A), consistent with previous observations in A. nidulans which showed NR activity rapidly disappeared from mycelial samples switched from NR induction media into media lacking a carbon source [35]. In M. oryzae, NR activity was also not detected in mycelia grown under nitrogen and carbon starvation conditions (-C –N, Figure 9A). Interestingly NR activity was detected in our M. oryzae mycelia grown in glucose minimal media lacking a nitrogen source (-N, Figure 9A). This is different to the observations by Hynes of A. nidulans NR activity [35], where absence of an inducer resulted in rapid loss of NR activity, but consistent with a previous M. oryzae report that showed NIA1 expression was elevated under nitrogen starvation conditions in M. oryzae compared to growth on nitrate media in the presence of glucose [36]. Figure S7A confirms that NIA1 is expressed in the absence of inducer, but not the absence of a carbon source, in wild type Guy11 strains. In addition, NIA1 is expressed in condia and appressoria in the absence of an inducer [23], and we show in Figure S7B that in appressoria, NIA1 expression is dependent on Tps1.
In A. nidulans, although NR activity requires an inducer, several other activities - such as acetamidase, histidase and formamidase - are present at high levels in nitrogen starvation media in the absence of an inducer. Todd et al [37] examined the expression of amdS to demonstrate for A. nidulans that under nitrogen starvation conditions, in the presence of glucose, AreA located to the nucleus. AreA nuclear accumulation was rapidly reversed by the addition of an exogenous nitrogen source, and was not seen in nitrogen starvation media lacking a carbon source. Our results might be consistent with this model of AreA/Nut1 activity in M. oryzae under at least some starvation conditions where NIA1 gene expression does not appear to require an inducer.
The model in Figure 8D suggests carbon metabolism and nitrogen metabolism are regulated by the Nmr1-3 inhibitor proteins in response to glucose, and Figure 9A and Figure S7A confirm NR activity and NIA1 gene expression is abolished in carbon starvation media in the presence of nitrate. However, the model in Figure 8D predicts that inactivating the Nmr1-3 inhibitor proteins should result in NIA1 gene expression in carbon starvation media. Consistent with this hypothesis, Figure 9B shows that NIA1 gene expression is significantly elevated in the Δnmr1 Δnmr2 Δnmr3 triple mutant [23] following growth on −C+NO3− media compared to Guy11.
The model also predicts that in Guy11, under carbon and nitrogen derepressing conditions (for example 55 mM xylose+10 mM NO3−), Nmr1-3 inhibitor proteins would be active, resulting in both Nut1 inhibition and CCR repression. The outcome of this growth condition is expected to be both decreased NIA1 expression and increased expression of genes for alternative carbon source utilization. Conversely, growth of the Δnmr1 Δnmr2 Δnmr3 triple mutant under the same conditions should result in increased NIA1 gene expression, and active CCR and decreased expression of alternative carbon utilization genes, relative to Guy11 (Figure 8D). Figure 9C shows this to be the case, with ICL1 gene expression reduced, and NIA1 gene expression elevated, in Δnmr1 Δnmr2 Δnmr3 triple mutant strains compared to Guy11 following growth on 55 mM xylose and 10 mM NO3−.
Taken together, these results support a role for Tps1 in integrating carbon and nitrogen metabolism such that in glucose-rich conditions, Tps1 senses G6P and inactivates Nmr1-3 regardless of nitrogen source, resulting in active Nut1 and CCR. Conversely, in the absence of G6P, Nmr1-3 would simultaneously repress CCR and nitrogen metabolism regardless of nitrogen source.
The role of Nmr1-3 inhibition in the expression of known virulence factors under nitrogen starvation conditions
In M. oryzae and other plant pathogens, it has been noted that virulence-associated gene expression is induced on glucose minimal media lacking a nitrogen source [36], [38], and Figure 8D suggests one mechanism by which these genes could be controlled during infection. To explore this further we looked at the expression of two genes essential for virulence and encoding the vacuolar serine protease Spm1 [36] and the plasma membrane protein Pth11 [39]. PTH11 gene expression had previously been shown to be under Tps1 control [21] and both PTH11 and SPM1 were shown to be elevated in expression under nitrogen starvation conditions compared to nitrate inducing conditions [21]. However, whether the expression of PTH11 and SPM1 was ammonium-repressible, and whether that repression occured via Nmr1-3 control of Nut1, was not known. Figure 9D shows therefore that in Guy11, both SPM1 and PTH11 gene expression is induced in NO3− media compared to NH4+ media (with 55 mM glucose in both cases), and that this induction is dependent on Nut1. Figure 9E shows that SPM1 and PTH11 are also regulated by the Nmr1-3 inhibitor proteins in response to glucose whereby expression of both genes is elevated in the Δnmr1 Δnmr2 Δnmr3 strain during growth on carbon starvation media in the presence of nitrate, compared to Guy11. Figure 9F summarizes the transcriptional data in Figure 8 and Figure 9 to show how carbon and nitrogen metabolism is integrated in response to G6P availability, and how this could provide a framework for understanding how known virulence genes, expressed under nitrogen starvation conditions, are regulated during infection.
An extragenic forward suppressor screen identified MDT1, encoding a MATE–family efflux pump, as an additional regulator of the CCR signal transduction pathway in M. oryzae
At the start of this study, nothing was known about the downstream target(s) of Tps1 and Nmr1-3 involved in CCR, or what additional factors constitute the CCR signaling pathway in M. oryzae. Because CREA deletion mutants can often not be obtained [25], we used our Δnut1 deletion strain in a forward genetics screen to identify components of CCR by selecting for extragenic suppressors of Δnut1 that were restored in their ability to utilize proline or glucosamine in the presence of glucose. Our rationale for defining CCR in M. oryzae lies in understanding how carbon metabolism is regulated in M. oryzae and how such nutrient adaptability contributes to pathogenicity during plant infection. Agrobacterium tumefaciens-mediated mutagenesis using the binary vector pKHt [40] randomly introduced T-DNA into the genome of a Δnut1 parental strain, and the resulting suppressor strains were selected for growth on minimal media containing 10 mM glucose with 10 mM proline or 10 mM glucosamine as nitrogen source. We obtained a total of six transformats on 10 mM glucose+10 mM proline (Δnut1 Supp 3121021–Δnut1 Supp 3121026) and one transformant on 10 mM glucose+10 mM glucosamine (Δnut1 Supp 312104). Three transformants selected on 10 mM glucoe+10 mM proline were lost due to Agrobacterium contamination before we were able to identify the disrupted gene. For the remaing four strains (Δnut1 Supp 312102, Δnut1 Supp 3121023, Δnut1 Supp 3121025 and Δnut1 Supp 312104), inverse PCR and the known T-DNA sequence [40] were used to identify genes that had been disrupted by T-DNA insertion in our suppressor strains. Interestingly, all four Δnut1 suppressor strains resulted from T-DNA insertions into the same 3′ coding region of MDT1 encoding a MATE-family efflux pump [27] (Table S3, Figure S8A). Specifically, all resulted from T-DNA insertions immediately 3′ to nucleotide 1503, except Δnut1 Supp 3121023 which resulted from insertion of T-DNA immediately 3′ to nucleotide 1502. The position of these insertions could indicate that all the suppressors generated were not independent transformants. However, Δnut1 Supp 312104 was selected on 10 mM glucose+10 mM glucosamine using different Guy11 mycelial samples to the suppressors selected on glucose+proline. In addition, previous reports of using Agrobacterium -mediated mutagenesis in Arabidopsis [41] and Magnaporthe [42] demonstrated nonrandom integration of T-DNA, and “hotspots” of integration were determined. Therefore, our suppressors could result either from multiple insertions of Agrobacterium T-DNA into a “hotspot” region within MDT1, or result from clones of one transformant isolated during selection. The suppressor strains generated in Table S3, regardless of the selection media used, were able to grow on both glucosamine and proline as nitrogen source compared to the Δnut1 parental strain, and we arbitrarily chose Δnut1 Supp 3121022 for further characterization. To confirm MDT1 as the suppressing locus in Δnut1 suppressor strains, a Δnut1 Δmdt1 double deletion strain was generated by homologous gene replacement of MDT1 in the Δnut1 background (Figure S6B). MDT1 was also deleted in Guy11 to generate a single Δmdt1 deletion strain that was subsequently complemented with the full length MDT1 coding region.
Figure 10A shows how both the Δnut1 Supp 3121022 suppressor strain and the Δnut1 Δmdt1 double deletion strain, like the Δnut1 parental strain, could not use nitrate as a sole nitrogen source. Unlike Δnut1 deletion strains, however, Δnut1 Supp 3121022 and Δnut1 Δmdt1 strains could grow on proline and glucosamine as nitrogen source, and were sensitive to 100 mM AA in 55 mM glucose+10 mM NH4+ minimal media (Figure 10B), indicating they were derepressed for carbon metabolism in the presence of glucose.
The Δmdt1 single mutant strains could grow on nitrate, glucosamine and proline as nitrogen sources, and were slightly more sensitive to glucose minimal media with 100 mM AA than Guy11. Δmdt1 strains were also reduced in growth on minimal media with 10 mM glucose+10 mM NH4+ (discussed below).
MDT1 encodes a predicted membrane-spanning protein and is expressed during appressoria development
MDT1 is a member of the Multidrug and Toxin Extrusion (MATE) gene family found in bacteria, archaea and eukaryotes [43], [44], [45]. They have a wide range of cellular substrates and function as fundamental transporters of metabolic and xenobiotic organic cations in kidneys [46]; transporters of organic anions such as citrate in plants [47]; and contribute to antimicrobial drug resistance and protection against ROS damage in bacteria [48]. In M. oryzae, the MDT1 locus, MGG_03123, is one of three loci encoding putative MATE-family efflux pumps, the other two being MGG_04182 and MGG_10534 [49]. MGG_03123 consists of 2510 nucleotides, has three predicted introns, and encodes a 748 amino acid protein. PSortII analysis predicted the gene product has a 73.9% chance of being localized to the plasma membrane, and a 26.1% chance of being localized to the endoplasmic reticulum. TMpred and PSIPRED analysis predicted the protein carries 12 membrane-spanning helices located in the C-terminal region of the protein. An EST corresponding to MGG_03123,10_GI3391884.f, was detected in appressorial stage specific cDNAs deposited at the M. oryzae community database (www.mgosdb.org/).
MDT1 is required for sporulation and plant infection but not appressorium formation
Disruption of MDT1 by T-DNA insertion or homologous recombination, in wild type or Δnut1 backgrounds, resulted in significant reductions in spore production on minimal media with 55 mM glucose and 10 mM NH4+ compared to the Guy11 and Δnut1 parental strains (Figure 11A). Sufficient spores were harvested from CM plates to show that after 24 hrs, spores of Δmdt1, Δnut1 Δmdt1 and Δnut1 Supp 321022 strains formed appressorium normally on hydrophobic surfaces compared to Guy11 (Figure 11B). However, despite forming appressoria, Δmdt1, Δnut1 Δmdt1 and Δnut1 Supp 321022 strains were unable to establish disease when inoculated onto rice leaves (Figure 11C). To ensure loss of pathogenicity was solely due to the loss of a functional MDT1 gene, we show Δmdt1 strains complemented with the full length MDT1 gene are restored for pathogenicity (Figure 11C). Thus MDT1 is not required for appressorium development but is essential for both full sporulation and rice blast disease and is a new determinant of virulence in M. oryzae.
MDT1 is involved in citrate efflux and carbon regulation
We sought to identify the likely function of Mdt1 in order to understand how a MATE-family efflux pump might regulate carbon metabolism and mediate the fungal-host plant interaction. Only one other MATE-family transporter had previously been described in fungi, Erc1 from S. cerevisiae, which functions to confer fungal resistance to the toxic methionine analog ethionine [50]. We observed that although Guy11 is sensitive to the addition of 50 mM ethionine to glucose minimal media, MDT1 disruption strains did not demonstrate increased susceptibility compared to Guy11, suggesting Mdt1 is not involved in ethionine efflux in M. oryzae (Figure S9A).
Considering Δnut1 Supp3121022 and Δnut1 Δmdt1 strains were carbon derepressed in the presence of glucose (Figure 10B), we sought to determine if Mdt1 might be involved in glucose uptake and phosphorylation in the cell. As noted previously, a class of carbon derepressed mutants of A. nidulans were found to result from defective glucose uptake [3] and were consequently resistant to both the toxic glucose analogue 2-deoxyglucose (2-DOG), and the toxic sugar sorbose [32] during growth under carbon derepressing conditions. When grown on carbon derepressing minimal media comprising 10 mM xylose and 10 mM NH4+ as sole carbon and nitrogen sources, we observed, however, that disruption of MDT1 in all backgrounds tested (wild type and Δnut1) did not confer resistance to 50 µg/mL 2-DOG or 5 mM sorbose in M. oryzae (Figure S9B). This suggests Mdt1 is not involved in glucose uptake in M. oryzae.
In bacteria, the MATE-family efflux protein NorM protects GO-deficient strains against the deleterious effects of exogenous reactive oxygen species (ROS) [48]. As M. oryzae transitions from the surface of the leaf to the underlying tissue, it encounters basal plant defense strategies in the form of a plant-derived oxidative burst, which the fungus needs to neutralize in order to establish infection [51]. We wondered if Mdt1 might play a similar role to NorM in protecting M. oryzae against ROS, and if loss of this protection in MDT1 disruption strains might result in the observed loss of pathogenicity. However, Figure S9C shows how Δnut1 Δmdt1 double mutant and Δmdt1 single mutant strains were not significantly more sensitive to oxidative stress than wild type, as evidenced by their ability to grow like wild type and Δnut1 parental strains on CM supplemented with 10 mM H2O2. In contrast, Des1 is an M. oryzae gene product necessary for neutralizing plant ROS during infection [51]. Δdes1 mutant strains are unable to detoxify plant ROS and are severely attenuated in growth on CM containing only 3 mM H2O2 compared to wild type. Therefore Figure S9C suggests that, compared to Des1, the role of Mdt1 in protecting the fungus against ROS during infection is very minor.
Considering the wide range of MATE substrates demonstrated in other organisms, additional roles for Mdt1 could also include conferring toxin resistance during growth in planta through the extrusion of plant-derived defense compounds from the fungal cell, such as has been reported previously in M. oryzae for the transmembrane ATP binding cassette (ABC) proteins, Abc1 [52] and Abc3 [53]. However, it should be noted that unlike Δabc1 and Δabc3 mutant strains, MDT1 disruption strains evince physiological defects and inactivation of CCR under glucose-rich conditions in the absence of the plant host. This suggests Mdt1 has a major physiological role in carbon metabolism and any additional role(s) it might have in mediating resistance to plant toxins during infection is likely to be a minor function of this efflux pump.
In Arabidopsis thaliana and rice (Oryza sativa), root-associated MATE-family transporters, AtFRD3 and OsFRDL1 respectively, are indirectly involved in cellular metal uptake and homeostasis [54]–[57]. MATE proteins likely do not transport metal ions directly but are proposed to secrete citrate that chelates extracellular metal ions and conditions their translocation into the cell by other systems [47], [56], [58]. MATE proteins involved in citrate efflux have also been described for sorghum [59], barley [60], maize [61] and wheat [47]. In addition, fungi have been shown to secrete citrate, where it is considered overflow metabolism similar to that seen during growth on excess glucose [62]. We first sought to determine if Mdt1 was involved in metal uptake in M. oryzae. Figure 12A demonstrates that compared to growth on minimal media containing 55 mM glucose and 10 mM NH4+, growth on the same media supplemented with ten-fold the normal concentration of zinc, but not copper or iron (not shown), significantly increased sporulation rates in Δnut1 Supp 312022, Δnut1 Δmdt1 double deletion and Δmdt1 single deletion strains, but not Guy11 or Δnut1 parental strains, following 12 days of growth. This suggests the MDT1 disruption strains were impaired in zinc uptake.
Next, to explore the role of zinc metabolism during infection, we generated a deletion mutant of ZAP1 (MGG_04456) in M. oryzae by homologous gene replacement. Zap1 is a transcription factor described in Saccharomyces cerevisiae that regulates the expression of genes encoding zinc uptake systems [63]. S. cerevisiae strains carrying mutations in this gene grow poorly on zinc-depleted media. To test whether MoZAP1 is an Sczap1 functional homologue that regulates zinc homeostasis and acquisition, we measured the sporulation rates of Δzap1-carrying strains of M. oryzae grown on minimal media with our standard concentration of zinc (1×Zn) and on minimal media with a 100-fold reduction in zinc (1∶100×Zn) (Figure S10A). Compared to Guy11 on the same media, sporulation of Δzap1 strains was significantly reduced on 100-fold reduced zinc media compared to standard media, thus demonstrating these mutant strains were likely impaired for zinc acquisition on zinc-depleted media. When applied to rice leaves, Δzap1 strains were determined to be fully pathogenic (Figure S10B), indicating the physiological effects of growth under zinc-limiting conditions demonstrated for Δzap1 is not deterimental to pathogenicity. However, because complete loss of growth and sporulation of Δzap1 strains on zinc-limiting media was not observed, other zinc acquisition systems must be operational in these strains, and their elucidation warrants further investigation.
Metal homeostasis in plant roots is dependent on MATE proteins that, in some cases, have been shown to extrude citrate. In addition, fungi excrete citrate during growth under excess glucose conditions [62]. To determine if Mdt1 was involved in citrate efflux, we grew MDT1 deletion strains and parental strains in CM for 48 hr then switched the mycelia to ammonium minimal media for 16 hr (Figure 12B). Δnut1 Supp 321022 was omitted from these studies due to uncertainty about the effects any additional unidentified T-DNA insertions might have on citrate efflux. After 16 hr, the filtrate was harvested and citrate exudate was quantified using LC-MS/MS. Under these conditions, media of the single Δmdt1 deletion mutant did not contain less citrate than Guy11. However, media of Δnut1 contained significantly more citrate than Guy11, while media of the Δnut1 Δmdt1 double mutant was reduced in citrate compared to both parental strains. Therefore on ammonium media, loss of Mdt1 leads to reduced citrate efflux in the Δnut1 background.
On nitrate minimal media, the Δmdt1 deletion mutant was shown to produce less citrate in the media than Guy11 (Figure 12C) suggesting it might therefore be necessary for citrate efflux under these growth conditions. Δnut1 and Δnut1 Δmdt1 were not analyzed in this media because they are essentially nitrogen starved on nitrate and might generate spurious results regarding citrate efflux. Interestingly, reduced Mdt1-dependent citrate efflux appeared to correlate with carbon derpression such that in Δmdt1 strains, ADH1 gene expression was not different to Guy11 during growth on minimal media with glucose and ammonium, but was significantly elevated compared to Guy11 on minimal media with glucose and nitrate (Figure 12D). Indeed, Figure 12E shows that on minimal media with 55 mM glucose and 10 mM NO3−, Δmdt1 strains were carbon derepressed in the presence of glucose and highly sensitive to 100 mM AA compared to Guy11. To determine if loss of Mdt1 function and reduced citrate efflux correlated with changes in other carbon metabolic processes, we looked at the expression of ICL1, PFK1 and FBP1 in Guy11 and Δmdt1 strains following growth on minimal media with 55 mM glucose and 10 mM NO3− (Figure 12F). Under these conditions (and similar to Δtps1 strains), Δmdt1 expressed genes for alternative carbon source metabolism (ICL1) and gluconeogenesis (FBP1) more highly than Guy11 but was reduced in the expression of the glycolytic gene PFK1. Therefore, extrusion of citrate by Mdt1 is context-dependent (ie dependent on growth nutrient conditions and genetic background) and is likely required during overflow metabolism to remove excess citrate from the cell, while loss of Mdt1 function in the same conditions results in CCR activation in the presence of glucose.
Taken together, we propose the major physiological role for Mdt1 during infection and growth is in mediating carbon metabolism via extrusion of citrate, thus contributing to in planta nutrient adaptation.
MDT1 is hypostatic to TPS1
Δmdt1 strains grew poorly on minimal media with 10 mM glucose (Figure 10A), but were not impaired in glucose uptake and phosphorylation (Figure S9B). They were, however, misregulated for genes associated with alternative carbon source utilization and assimilation (Figure 12D and 12F). In addition, Figure 13A demonstrates that growth of Δmdt1 was improved on minimal media with 55 mM glucose compared to 10 mM glucose, while growth of Guy11 on either media is not affected. These results are similar to those seen for Δtps1, suggesting Mdt1 also functions to regulate glucose metabolism. To determine the genetic relationship between Tps1 and Mdt1, we constructed a Δtps1 Δmdt1 double mutant and compared its growth to Δmdt1 and Δtps1 single deletion strains. Growth of Δmdt1, but not Δtps1 Δmdt1 or Δtps1 strains, on minimal media with 55 mM glucose and 10 mM NO3− (Figure 13B) confirms that Mdt1 regulates CCR downstream of Tps1 and after the Nmr1-3 mediated pathway branch to nitrogen metabolism. In addition, Δtps1 Δmdt1 double deletion strains, like Δtps1, were highly sensitive to 100 mM AA on minimal media with ammonium compared to Δmdt1 single mutant strains, suggesting Tps1- and Mdt1- dependent regulation of CCR can occur in response to different signals, demonstrated in Figure 13C. In this model, in the presence of glucose, CCR is active due to G6P sensing by Tps1 and the inactivation of the Nmr1-3 inhibitor proteins. Under conditions of excess glucose, such as might be found in the photosynthesizing leaf, overflow metabolism results in citrate production, which is extruded from the cell by Mdt1. We propose perturbed citrate efflux from the cell can directly or indirectly inactivate CCR, slowing the uptake and metabolism of glucose and providing a mechanism for reducing overflow metabolism. Finally, as disease progresses and external glucose is exhausted, loss of G6P sensing by Tps1 inactivates CCR to allow the metabolism and assimilation of alternative carbon sources, such as cell wall polysacharides. Thus together, Tps1 and Mdt1 represent sensitive monitors of carbon metabolism that allow the fungus to adapt to fluctuating qualities and quantities of carbon sources during infection.
Conclusions and significance
Recently, Tps1 was shown in the rice blast fungus to integrate nitrogen metabolism with G6P availability, and we sought to determine what role it might play in regulating carbon utilization during infection. Here we demonstrate that Tps1, via the Nmr1-3 inhibitor proteins, regulates CCR in the presence of G6P to ensure the preferential utilization of glucose over less favourable carbon compounds. This confirms that in filamentous fungi, glucose phosphorylation, rather than signaling by individual hexokinase proteins, is the first step in signaling glucose repression [11]. Moreover, identification of Tps1 and Nmr1-3 as regulators of CCR re-iterates how carbon and nitrogen metabolism is intimately linked in M. oryzae, a likely necessity for its plant pathogenic lifestyle. More work is needed to understand the dynamics of the Nmr inhibitor proteins with their targets, and the identity of those targets. In nitrogen metabolism, all three Nmr proteins converge on Nut1 to repress its activity (including Nmr2 which was not shown to physically interact with Nut1 in yeast two-hybrid studies) [23], whereas the work presented here suggests that in carbon metabolism, each Nmr protein might repress different co-activators of CCR such that taking out any one of the Nmr proteins alleviates repression of its cognate target and activates CCR. Determining the identity of these targets is important because, similarly, deleting any one of the NMR genes in Δtps1 strains restores virulence [23], suggesting the targets of Nmr1-3 in CCR and in pathogenicity might be similar. More work is needed to identify these targets of Nmr1-3, and Co-IP pull-down experiments will be conducted to identify them. In the future we intend to continue our identification of other components of CCR, and will also undertake the functional characterization of a likely M. oryzae homologue of CreA, MGG_11201. creA− mutant strains were not isolated in our Agrobacterium-mediated mutagenesis screen, and targeted deletion of this gene will be undertaken to determine its role in glucose metabolism and infection in M. oryzae. In addition, in the event deletion of MGG_11201 is lethal, we will also attempt a gene silencing approach to eliminate MoCreA from different points in the fungal lifecycle. Intriguingly, MGG_11201 gene expression appears under Tps1-control (Hartline and Wilson, unpublished results) and we intend to explore the relationship between Tps1 and MGG_11201 in the future.
Selecting for extragenic suppressors of our available mutant strains, we determined that a MATE-family efflux protein, Mdt1, is an additional regulator of CCR that is necessary for sporulation and essential for virulence. This is the first time a MATE-family protein has been characterized in either a filamentous fungus or a plant pathogen. In addition to identifying a novel pathogenicity factor, this is also the first study to assign a genetic regulatory role to a MATE-family efflux protein. Understanding the function of MATE proteins is clinically important due to their role in multi drug resistance, where bacterial MATE transporters reduce the efficacy of antibiotic treatments by extruding those drugs that resemble native substrates [44], [64], [65]. MATE proteins also influence the pharmacokinetics of therapeutic drug regimes in a similar manner [66]–[68], for instance by affecting the treatment of diabetes through the extrusion of the glucose-lowering drug Metformin [69]. The work described here could serve as a model for understanding the physiological role of these transporters, thus helping to identify their native substrates and contributing to a better understanding of how treatments impacted by MATE proteins could be improved. Moreover, the essential role of Mdt1, a putative transmembrane pump, in plant pathogenesis and sporulation makes it a superb and accessible target for future anti-rice blast strategies.
Fungi posses sensitive gene regulatory mechanisms for responding to nutrient fluctuations in the environment, but until recently little was known about these systems in pathogens such as the devastating rice blast fungus M. oryzae. Such mechanisms must be essential in M. oryzae for three reasons: they would signal the transition of the fungus from the nutrient-free surface to the sugar-rich interior of the host; they would allow the fungus to respond rapidly to the nutritional status of the host; and they would temper the voracious appetite of M. oryzae during the biotrophic growth stage in the plant. M. oryzae can utilize a wide range of carbon sources in plate tests ([20]; Quispe and Wilson, unpublished), but in planta growth is rigorously controlled and choreographed during the early stages of infection, with the fungus residing in one cell for 8–12 hr before moving to the next in a biotrophic and symptomless manner [18]. Only later does the fungus enter its necrotic phase, causing plant tissue destruction and escape of the fungal spores from the host. From our data it is likely CCR contributes to the spatial and temporal regulation of M. oryzae development during infection. The work described here suggests a scenario whereby Tps1 and Mdt1 regulate CCR to optimize growth under the changing glucose conditions likely found during ramification throughout the epidermal and mesophyl layer; during the leaf photosynthetic cycle; and during the necrotic phase when leaf cells are destroyed, photosynthesis ceases and G6P levels drop. Figure 14A demonstrates that controling CCR is relevant to the infection process because ICL1, which is misregulated in Δtps1 and Δmdt1 strains, is not expressed by the wild type until the appearance of necrotic lesions. CCR control of CWDEs is also likely to be important for the pathogenicity of M. oryzae, and Figure 14B shows that Δmdt1 strains, like Δtps1 strains, are misregulated for CWDE gene expression. Analysis of the genome of the obligate biotrophic plant pathogens Ustilago maydis [70] and Blumeria graminis [71] reveal they carry a marked reduction in genes encoding CWDEs compared to other plant pathogens, suggesting CWDEs are not required - and may be detrimental - to the biotrophic lifestyle. Therefore, we propose misregulation of CCR in Δtps1 and MDT1 disruption strains during the early biotrophic stages of infection is likely to have profound effects on the ability of M. oryzae to establish disease – perhaps in part due impaired glucose assimilation and the perturbed expression of CWDEs.
Identifying the interplay of regulatory systems that condition M. oryzae nutrient acquisition and growth in the plant, and how that control can be perturbed, is an ongoing future goal of our research.
Materials and Methods
Strains, physiological tests, and plant infections
All strains used in this study were derived from Guy11 (Table S4). Strains were grown on complete medium (CM) containing 1% (W/V) glucose, 0.2% (W/V) peptone, 0.1% (W/V) yeast extract and 0.1% (W/V) casamino acids, or on minimal medium (MM) containing 1% glucose and 0.6% sodium nitrate, unless otherwise stated, as described in [21]. 55 mm petri dishes were used unless stated otherwise. Allyl alcohol (ACROS organics, USA), kanamycin (Fisher, USA), sorbose (Sigma, USA), 2-deoxyglucose (Sigma, USA) and ethionine (Sigma, USA) were added to CM or MM in the amounts indicated. Plate images were taken with a Sony Cyber-shot digital camera, 14.1 mega pixels. Nitrate reductase enzyme activity was measured as described previously [21]. For spore counts, 10 mm2 blocks of mycelium were transferred to the centre of each plate, and the strains grown for 12 days at 26°C with 12 hr light/dark cycles. Spores harvested in sterile distilled water, vortexed vigorously and counted on a haemocytometer (Corning). Spores were counted independently at least four times. Rice plant infections were made using a susceptible dwarf Indica rice (Oryza sativa) cultivar, CO-39, as described previously [23]. Fungal spores were isolated from 12–14 day-old plate cultures and spray-inoculated onto rice plants of cultivar CO-39 in 0.2% gelatin at a concentration of 5×104 spores/ml, unless otherwise stated, and disease symptoms were allowed to develop under conditions of high relative humidity for 96–144 hrs.
Gene transcript analysis
For fungal gene transcript studies, strains were grown for 48 h in CM before switching to minimal media for 16 hr, unless otherwise stated. Mycelia was harvested, frozen in liquid nitrogen, and lyophilised overnight. For leaf RNA extractions, tissues were weighed and approximately 100 mg of tissue was frozen in liquid nitrogen and ground in a mortar and pestle. RNA was extracted from fungal mycelium and infected leaf tissue usng the RNeasy mini kit from Qiagen. RNA was converted to cDNA using the qScript reagents from Quantas. Real time quantitative PCR was performed on an Eppendorf Mastercycler Realplex using the recommended reagents with primers designed using the netprimer software program (Table S5). qPCR data was analyzed using the Realplex software. Thermocycler conditions were: 10 min at 95°C, followed by 40 cycles of 95°C for 30 sec, 63°C for 30 sec and 72°C for 30 sec.
Agrobacterium-mediated transformation of Magnaporthe oryzae
A. tumefaciens-mediated transformation was performed as previously described [72] by incubating the Agrobacterium strain ALG1 containing the binary vector pKHt [40] with 0.5–1.0 g of fungal mycelia grown as a liquid shake in CM.
Proline and glucosamine selection plates were prepared by first pouring a 3–5 mm thick support layer of minimal media without any carbon or nitrogen source. Celluose nitrate membranes containing co-incubated Agrobacterium and Magnaporthe strains were laid on top of this support layer and the metabolic selection was then poured over the co-incubation membranes. The selection media contained glucose with proline or glucosamine plus 250 µg/ml hygromycin (CalBiochem), 400 µg/ml cefotaxime (Research Products International Corp), 100 µg/ml carbenicillin (Fisher BioReagents), and 60 µg/ml streptomycin (Fisher BioReagents). These antibiotics both kill the Agrobacterium and select for hygromycin insertion. Following standard incubation conditions, colonies appeared in 5–10 days and were transferred to a purification plate containing the appropriate carbon and nitrogen sources and the antibiotics described above.
To identify which gene was mutated by T-DNA insertion, DNA was extracted from purified colonies as described previously [73]. DNA sequences flanking the right border of the T-DNA inserts were amplified by inverse PCR [74]. Genomic DNA was digested with BamHI (Fermentas), ligated to circularize the products using T4 DNA Ligase (NEB), and amplified by PCR using primers designed from the known sequence of the Hph gene, conferring hygromycin resistance, present in the T-DNA insert (Table S5). PCR conditions were 1 min at 95°C followed by 35 cycles of 30 sec at 95°C, 30 sec at 63°C and 3 min at 68°C. PCR products were subcloned into pGEM-T (Promega), transformed into JM109 competent cells (Promega) and sequenced by Eurofins MWG Operon, USA.
Genetic manipulations
Targeted gene replacement was achieved by the split marker method described in [23] using the oligonucleotide primers shown in Table S5. Δnut1 was generated in Guy11 and Δtps1 parental strains using the ILV1 gene conferring sulphonyl urea resistance as the selectable marker. MDT1 was deleted in all the strains studied using the bar gene conferring bialaphos resistance. The hexose phoshorylase genes HXK1, HXK2 and GLK1 were deleted in Guy11 using the bar gene conferring bialaphos resistance resistance as the selectable marker. ZAP1 was deleted in Guy11 using the ILV1 gene conferring sulphonyl urea resistance as the selectable marker. Gene deletions were verified by PCR as described previously [23]. The role of MDT1 in pathogenicity was verified by complementation through the introduction of a plasmid carrying MDT1 into the Δmdt1 deletion strain. Resulting complementation strains were tested for pathogenicity on rice leaves. The full length MDT1 complementation vector was constructed usng the primer pairs shown in Table S5 and following the protocol of Zhou et al [75].
Protein extraction and LC/MS/MS analysis
Proteomic studies were performed as follows. Strains were grown in CM for 48 hrs, then transferred to minimal media with nitrate for 16 hrs, following [23]. 500 mg of fungal biomass (wet weight) was transferred in to a 1 ml lysis buffer comprising 8 M urea in 100 mM ammonium bicarbonate and containing 1.5 mM protease inhibitor (PMSF, Sigma). The biomass was then subjected to bead beating using a glass bead beater for 3 minutes at 4°C. The supernatant was collected after centrifugation at 10000 g for 20 minutes. The protein in the supernatant was collected by cold acetone precipitation (1 ml of sample added with 9 ml of acetone) overnight at −2°C. The resultant precipitate was collected by centrifugation at 7000 g for 30 minutes. The precipitate was air dried to remove residual acetone and the dried pellets were resolubilised in 250 µl of 100 mM ammonium bicarbonate. The protein concentration was estimated using the BCA protein assay kit (Thermo Fisher Scientific).
For the LC/MS/MS experiments, in solution trypsin digestion was performed on the extracted fungal protein using the protocol described in [76]. Following 16 hours of tryptic digestion the reaction was terminated by adding 0.1% formic acid. The peptide solution was dried using a speed vacuum drier (Thermo fisher) and reconstituted with 20 µl of 0.1% formic acid in water. These peptides were later subjected to LC/MS/MS analysis with an ultimate 3000 Dionex nano LC system (Dionex corporation) integrated with LCQ Fleet Ion Trap mass spectrometer (Thermo scientific) equipped with a nano source. The acquired MS/MS spectrum was searched against the Magnaporthe oryzae protein sequence database (NCBI) using MASCOT (Matrix Sciences, UK) bioinformatics software to identify the protein and Scaffold software (Proteome Software Inc., USA) for further spectrum counting and relative protein quantification analysis.
Metabolite analysis
The citrate in the fungal culture broth was measured using liquid chromatography mass spectrometry in SRM mode (Single reaction mode). 10 µl of the centrifuged culture broth sample was injected into the LC-MS/MS system. An LC system (Agilent 1200 series HPLC) was integrated with a C18 column (50×2.1 mm, Thermo Fisher GOLD) with a flow rate of 0.3 ml/min water containing 2 mmol l−1 ammonium acetate and 0.1% (v/v) formic acid for loading the samples. A step gradient of 100% Acetonitrile containing 2 mmol l−1 ammonium acetate and 0.1% (v/v) formic acid was used to wash the column. The citrate eluted from the column isocratically with a retention time of 0.52 min. The analytes were monitored using a triple quadruple mass spectrometer (AB SCIEX Q Trap 4000) operated in multiple reaction monitoring mode using the following transition: citrate m/z 193.10>175.0. An external calibration was set using the same conditions using pure citrate (Sigma, USA) and peak area was calculated for quantitation purpose using the Analyst 1. 5.1 software (AB SCIEX). The unknown concentration of the citrate was calculated from the obtained calibration curve.
Supporting Information
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Genetika Reprodukční medicínaČlánek vyšel v časopise
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