Phosphatidylserine synthetase regulates cellular homeostasis through distinct metabolic mechanisms


Autoři: Xiao Yang aff001;  Jingjing Liang aff001;  Long Ding aff001;  Xia Li aff001;  Sin-Man Lam aff004;  Guanghou Shui aff001;  Mei Ding aff001;  Xun Huang aff001
Působiště autorů: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China aff001;  University of Chinese Academy of Sciences, Beijing, China aff002;  School of Life Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, TaiAn, China aff003;  LipidAll Technologies Co., Ltd. Changzhou, China aff004
Vyšlo v časopise: Phosphatidylserine synthetase regulates cellular homeostasis through distinct metabolic mechanisms. PLoS Genet 15(12): e32767. doi:10.1371/journal.pgen.1008548
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008548

Souhrn

Phosphatidylserine (PS), synthesized in the endoplasmic reticulum (ER) by phosphatidylserine synthetase (PSS), is transported to the plasma membrane (PM) and mitochondria through distinct routes. The in vivo functions of PS at different subcellular locations and the coordination between different PS transport routes are not fully understood. Here, we report that Drosophila PSS regulates cell growth, lipid storage and mitochondrial function. In pss RNAi, reduced PS depletes plasma membrane Akt, contributing to cell growth defects; the metabolic shift from phospholipid synthesis to neutral lipid synthesis results in ectopic lipid accumulation; and the reduction of mitochondrial PS impairs mitochondrial protein import and mitochondrial integrity. Importantly, reducing PS transport from the ER to PM by loss of PI4KIIIα partially rescues the mitochondrial defects of pss RNAi. Together, our results uncover a balance between different PS transport routes and reveal that PSS regulates cellular homeostasis through distinct metabolic mechanisms.

Klíčová slova:

Cell growth – Cell membranes – Drosophila melanogaster – Lipids – Mitochondria – RNA interference – Salivary glands – Hormone transport


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Štítky
Genetika Reprodukční medicína

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PLOS Genetics


2019 Číslo 12

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