Drosophila RpS12 controls translation, growth, and cell competition through Xrp1


Autoři: Zhejun Ji aff001;  Marianthi Kiparaki aff001;  Virginia Folgado aff001;  Amit Kumar aff001;  Jorge Blanco aff001;  Gerard Rimesso aff001;  Jacky Chuen aff001;  Yang Liu aff001;  Deyou Zheng aff001;  Nicholas E. Baker aff001
Působiště autorů: Department of Genetics, Albert Einstein College of Medicine, Bronx, New York, United States of America aff001;  The Saul R. Korey Department of Neurology, Albert Einstein College of Medicine, Bronx, New York, United States of America aff002;  Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, United States of America aff003;  Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York, United States of America aff004;  Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, Bronx, New York, United States of America aff005
Vyšlo v časopise: Drosophila RpS12 controls translation, growth, and cell competition through Xrp1. PLoS Genet 15(12): e32767. doi:10.1371/journal.pgen.1008513
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008513

Souhrn

Whereas complete loss of Rp function is generally lethal, most heterozygous Rp mutants grow more slowly and are subject to competitive loss from mosaics tissues that also contain wild type cells. The rpS12 gene has a special role in the cell competition of other Ribosomal Protein (Rp) mutant cells in Drosophila. Elimination by cell competition is promoted by higher RpS12 levels and prevented by a specific rpS12 mis-sense mutation, identifying RpS12 as a key effector of cell competition due to mutations in other Rp genes. Here we show that RpS12 is also required for other aspects of Rp mutant phenotypes, including hundreds of gene expression changes that occur in ‘Minute’ Rp heterozygous wing imaginal discs, overall translation rate, and the overall rate of organismal development, all through the bZip protein Xrp1 that is one of the RpS12-regulated genes. Our findings outline the regulatory response to mutations affecting essential Rp genes that controls overall translation, growth, and cell competition, and which may contribute to cancer and other diseases.

Klíčová slova:

Gene expression – Gene regulation – Imaginal discs – Messenger RNA – Point mutation – Protein translation – Ribosomes


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Genetika Reprodukční medicína

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PLOS Genetics


2019 Číslo 12

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