Response to the Formal Letter of Z. Chrzanowska-Lightowlers and R. N. Lightowlers Regarding Our Article “Ribosome Rescue and Translation Termination at Non-Standard Stop Codons by ICT1 in Mammalian Mitochondria”


article has not abstract


Published in the journal: . PLoS Genet 11(6): e32767. doi:10.1371/journal.pgen.1005218
Category: Formal Comment
doi: 10.1371/journal.pgen.1005218

Summary

article has not abstract

Introduction

Most deviations from the universal genetic code exist in the mitochondrial translation system. In human mitochondria, two arginine codons, AGA and AGG, have no cognate tRNAs; mtDNA-encoded cytochrome c oxidase subunit I (MTCO1) and NADH dehydrogenase 6 (MTND6) carry AGA and AGG codons at the end of their mRNAs, respectively. We previously demonstrated in vitro the possible engagement of ICT1 in the translation termination at non-standard stop codons of MTCOI and MTND6 mRNAs. On the other hand, Temperley and colleagues proposed in 2010 that human mitoribosomes invoke a -1 frameshift at the terminal AGA/AGG codons placing standard UAG stop codon in the ribosomal A-site. As consequence, only a single release factor, mtRF1a/RF1Lmt, would be used in mitochondria. Here we revisit the frameshift model and explain the view that ICT1 is presently a plausible candidate for the termination factor for non-standard stop codon in human mitochondria.

Response

We previously demonstrated in vitro that the mitochondrial factor ICT1 is possibly involved in the translation termination at non-standard termination codons AGA and AGG of two mitochondrial ORFs, MTCOI and MTND6, respectively [1]. These findings challenged the in vivo data by Temperley et al., who proposed a -1 frameshift at the terminal AGA and AGG codons in MTCOI and MTND6 [2]. As a consequence, both ORFs terminate in the standard UAG codon, thus using only a single release factor, mtRF1a/RF1Lmt, in mitochondria [3,4]. This model would imply that ICT1 is not necessary and is not involved in the termination of MTCOI and MTND6, since mtRF1a/RF1Lmt acts more predominantly than ICT1 at the standard UAG stop codon [1]. Here we would like to revisit the frameshift model and the mechanism of translation termination at non-standard stop codon in human mitochondria.

Bacterial RelE is thought to show sequence preference for standard termination codons UAG and UAA and negligible recognition of AGA and AGG

An elegant technique was applied, demonstrating -1 frameshifting in human mitochondria: the bacterial RelE, an endoribonuclease that specifically cleaves mRNA in the ribosomal A-site, was targeted to mitochondria (mtRelE) [2]. In vitro studies revealed that RelE induced cleavage most commonly after the second nucleotide of the A-site codon [5]. The mtRelE cleavage occurred in MTCO1 mRNAs uniquely between nucleotides A and G (U A|GA), suggesting a -1 frameshift with the UAG codon at the A-site.

There are two problems with this interpretation. (i) An extensive in vivo study analyzed 196 cleavage sites and observed a very modest sequence and site specificity: slightly more cuts were observed after a codon than between the second and third nucleotide of an A-site codon; 64% of the cleaved codons ended with a G or C, in 40% of the cases the cleavage occurred before or after a G [6]. In vitro study demonstrated that the bacterial RelE cleaves mRNA with high codon specificity with preference for the stop codon UAG, while the recognition of AGA codon is below the detection limit [5]. Structural study revealed that binding of RelE to ribosomal A-site reorganizes the mRNA of the A-site. Stacking of A-site codon bases with conserved residues in RelE and 16S rRNA explains the sequence specificity of the reaction, preference for pyrimidines in the first position of the codon, and purines in positions two and three [5,7]. Altogether, these data suggest that a RelE cleavage pattern cannot be taken as a proof for an A-site location of a codon as Temperley et al. do [2]. (ii) A -1 frameshift will only shift a UAG stop codon into the A-site, if the terminal AGA/AGG codon is preceded by a U nucleotide. This is not the case in most of the vertebrates, i.e., a -1 frameshift in the mitochondrial mRNAs is not universal. Furthermore, in Oryctolagus cuniculus (rabbit) the two terminal codons for CYTB and ND6 are UGA AGG and GCU AGG [8]; should in the first case ICT1 make the termination job, and in the second a -1 frameshift in cooperation with RF1Lmt/mtRF1a?

We think it would be important first to examine the codon specificity of RelE on 55S mitoribosome, before it is established that human mitoribosomes invoke frameshifting.

The elements that promote -1 frameshift are ambiguously identified in COI and ND6 mRNAs

Particular elements that promote classical frameshifting have been well characterized in other systems [911]. These include (i) a ribosome that has paused or stalled as a consequence of either a rare or slowly decoded codon, (ii) an upstream heptapeptide “slippery sequence” followed by an inhibitory secondary structure downstream, and (iii) only an inhibitory secondary structure downstream from the frameshift site. In this regard, Temperley and colleagues invoked the following features that should provoke a -1 frameshifting in MTCOI/MTND6 mRNA: (a) mitoribosomes stalling at AGA/AGG codons for which there are no cognate mt-tRNAs, potentially in concert with (b) diminished upstream codon/anticodon interactions due to a “non-conventional E-site,” and (c) a secondary structure immediately downstream (see supporting online material for [2]).

Limited information for the structure of mitoribosome was available when the frameshifting model was proposed. At that time, no tRNA had been found at the putative E-site of the 55S mitoribosome, and it was claimed that a conventional E-site does not exist in mitoribosomes, reducing both the number and hence overall strength of codon/anticodon interactions [12,13]. Recent structural analyses of mitoribosome, however, revealed that the binding pocket for the CCA-3’ end of the E-site tRNA observed in bacteria is well conserved in the 39S mitoribosomal large subunit and in a similar conformation compared to the occupied bacterial E-site [14]. An endogenous tRNA that co-purified with the mitoribosome can be seen in the E-site, along with the L1 stalk that interacts with it [15]. The 55S mitoribosome contains a conventional E-site, whereas elements corresponding to the slippery sequence are still obscure.

Both MTCOI and MTND6 mRNA seem to contain a secondary structure immediately downstream of AGA/AGG codons. The terminal AGA of MTCOI mRNA is followed by the antisense of tRNAser (UCN), and the AGG of MTND6 mRNA is followed by UTRs that extend into the antisense of MTND5 [16]. Folding algorithms predict the antisense tRNAser (UCN) to generate a stable cloverleaf similar to a tRNA, and the sequence immediately downstream of MTND6 to form a stem loop (RNAfold Web Server, http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi). It is of note here that these sequences immediately downstream of AGA/AGG codons cannot fold into structure when the ribosome is paused at AGA/AGG codon. Accordingly, it is still unclear whether a secondary structure immediately downstream of AGA/AGG codons functions to promote -1 frameshifting.

At present, ICT1 is the only factor that has been shown to exhibit a peptide release activity at non-standard stop codons AGA and AGG. The structural model of ICT1 has also supported this peptide release activity of ICT1 at non-standard stop codons [8]. The integrated ICT1 is located at the base of the central protuberance >70 Å away from the A-site [14,15,17] and, therefore, cannot act as a termination factor at the A-site. It is still an exciting riddle, whether or not the integrated ICT1 is released from the ribosome under distinct condition to fulfill its termination function. A similarly exciting question is the role of the other members of the mitochondrial RF family, namely mtRF1 and C12orf65. It is still possible that mtRF1 and/or C12orf65 are involved in the termination of non-standard stop codons in mammalian mitochondria.


Zdroje

1. Akabane S, Ueda T, Nierhaus KH, Takeuchi N (2014) Ribosome rescue and translation termination at non-standard stop codons by ICT1 in mammalian mitochondria, PLOS genetics. 10, e1004616. doi: 10.1371/journal.pgen.1004616 25233460

2. Temperley R, Richter R, Dennerlein S, Lightowlers RN, Chrzanowska-Lightowlers ZM (2010) Hungry codons promote frameshifting in human mitochondrial ribosomes, Science. 327, 301.

3. Soleimanpour-Lichaei HR, Kuhl I, Gaisne M, Passos JF, Wydro M, et al. (2007) mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG, Molecular cell. 27, 745–57.

4. Nozaki Y, Matsunaga N, Ishizawa T, Ueda T, Takeuchi N (2008) HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG, Genes to cells: devoted to molecular & cellular mechanisms. 13, 429–38.

5. Pedersen K, Zavialov AV, Pavlov MY, Elf J, Gerdes K, et al. (2003) The bacterial toxin RelE displays codon-specific cleavage of mRNAs in the ribosomal A site, Cell. 112, 131–40.

6. Hurley JM, Cruz JW, Ouyang M, Woychik NA (2011) Bacterial toxin RelE mediates frequent codon-independent mRNA cleavage from the 5' end of coding regions in vivo, The Journal of biological chemistry. 286, 14770–8. doi: 10.1074/jbc.M110.108969 21324908

7. Neubauer C, Gao YG, Andersen KR, Dunham CM, Kelley AC, et al. (2009) The structural basis for mRNA recognition and cleavage by the ribosome-dependent endonuclease RelE, Cell. 139, 1084–95. doi: 10.1016/j.cell.2009.11.015 20005802

8. Lind C, Sund J, Aqvist J (2013) Codon-reading specificities of mitochondrial release factors and translation termination at non-standard stop codons, Nature communications. 4, 2940. doi: 10.1038/ncomms3940 24352605

9. Gesteland RF, Weiss RB, Atkins JF (1992) Recoding: reprogrammed genetic decoding, Science. 257, 1640–1. 1529352

10. Olubajo B, Taylor EW, (2005) A -1 frameshift in the HIV-1 env gene is enhanced by arginine deficiency via a hungry codon mechanism, Mutation research. 579, 125–32. 16055159

11. Kerrigan JJ, McNulty DE, Burns M, Allen KE, Tang X, et al. (2008) Frameshift events associated with the lysyl-tRNA and the rare arginine codon, AGA, in Escherichia coli: a case study involving the human Relaxin 2 protein, Protein expression and purification. 60, 110–6. doi: 10.1016/j.pep.2008.02.016 18474430

12. Sharma MR, Koc EC, Datta PP, Booth TM, Spremulli LL, et al. (2003) Structure of the mammalian mitochondrial ribosome reveals an expanded functional role for its component proteins, Cell. 115, 97–108.

13. Mears JA, Sharma MR, Gutell RR, McCook AS, Richardson PE, et al. (2006) A structural model for the large subunit of the mammalian mitochondrial ribosome, Journal of molecular biology. 358, 193–212. 16510155

14. Greber BJ, Boehringer D, Leibundgut M, Bieri P, Leitner A, et al. (2014) The complete structure of the large subunit of the mammalian mitochondrial ribosome, Nature. 515, 283–6. doi: 10.1038/nature13895 25271403

15. Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, et al. (2014) Structure of the large ribosomal subunit from human mitochondria, Science. 346, 718–22. doi: 10.1126/science.1258026 25278503

16. Ojala D, Montoya J, Attardi G (1981) tRNA punctuation model of RNA processing in human mitochondria, Nature. 290, 470–4. 7219536

17. Greber BJ, Boehringer D, Leitner A, Bieri P, Voigts-Hoffmann F, et al. (2014) Architecture of the large subunit of the mammalian mitochondrial ribosome, Nature. 505, 515–9.

Štítky
Genetika Reprodukční medicína

Článek vyšel v časopise

PLOS Genetics


2015 Číslo 6

Nejčtenější v tomto čísle

Tomuto tématu se dále věnují…


Kurzy

Zvyšte si kvalifikaci online z pohodlí domova

Cesta pacienta s CHOPN
nový kurz
Autoři: doc. MUDr. Vladimír Koblížek, Ph.D.

Arteriální hypertenze ve světle nových evropských guidelines
Autoři: MUDr. Libor Jelínek

Precizní medicína v onkologii
Autoři:

Kožní toxicita cílené terapie inhibitory EGFR a VEGF
Autoři: MUDr. Karolína Svobodová

Jak na psoriázu v každodenní ambulantní praxi?
Autoři: MUDr. Jan Šternberský, Ph. D.

Všechny kurzy
Kurzy Doporučená témata Časopisy
Přihlášení
Zapomenuté heslo

Nemáte účet?  Registrujte se

Zapomenuté heslo

Zadejte e-mailovou adresu se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.

Přihlášení

Nemáte účet?  Registrujte se