Drosophila Myc restores immune homeostasis of Imd pathway via activating miR-277 to inhibit imd/Tab2


Autoři: Ruimin Li aff001;  Hongjian Zhou aff001;  Chaolong Jia aff001;  Ping Jin aff001;  Fei Ma aff001
Působiště autorů: Laboratory for Comparative Genomics and Bioinformatics & Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China aff001
Vyšlo v časopise: Drosophila Myc restores immune homeostasis of Imd pathway via activating miR-277 to inhibit imd/Tab2. PLoS Genet 16(8): e32767. doi:10.1371/journal.pgen.1008989
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008989

Souhrn

Drosophila Myc (dMyc), as a broad-spectrum transcription factor, can regulate the expression of a large number of genes to control diverse cellular processes, such as cell cycle progression, cell growth, proliferation and apoptosis. However, it remains largely unknown about whether dMyc can be involved in Drosophila innate immune response. Here, we have identified dMyc to be a negative regulator of Drosophila Imd pathway via the loss- and gain-of-function screening. We demonstrate that dMyc inhibits Drosophila Imd immune response via directly activating miR-277 transcription, which further inhibit the expression of imd and Tab2-Ra/b. Importantly, dMyc can improve the survival of flies upon infection, suggesting inhibiting Drosophila Imd pathway by dMyc is vital to restore immune homeostasis that is essential for survival. Taken together, our study not only reports a new dMyc-miR-277-imd/Tab2 axis involved in the negative regulation of Drosophila Imd pathway, and provides a new insight into the complex regulatory mechanism of Drosophila innate immune homeostasis maintenance.

Klíčová slova:

Drosophila melanogaster – Escherichia coli infections – Homeostasis – Immune response – Luciferase – MicroRNAs – Regulator genes – Transcriptional control


Zdroje

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