and Hyperdrive Mechanisms (in Mouse Meiosis)

article has not abstract

Published in the journal: . PLoS Genet 11(2): e32767. doi:10.1371/journal.pgen.1004950
Category: Perspective
doi: 10.1371/journal.pgen.1004950


article has not abstract

“Mendelism is a magnificent invention for fairly testing genes in many combinations, like an elegant factorial experimental design. Yet it is vulnerable at many points and is in constant danger of subversion by cheaters that seem particularly adept at finding such points.”

- James F. Crow [1]

Mendelian transmission is established during meiosis, the cell division that generates haploid gametes (e.g., sperm and eggs) from diploid germ cells. Meiosis does not, however, have to be fair. Selfish genetic elements, or meiotic drivers, have evolved to cheat this process in order to be packaged into functional gametes more often than the expected 50% probability. By biasing allele transmission in their favor, meiotic drive alleles can short-circuit natural selection, causing their spread even if they are harmful to organismal fitness. Indeed, meiotic drive alleles are thought to be directly or indirectly associated with infertility in diverse eukaryotes, from fungi to flies to humans [2]. Drive occurring in male meiosis may have immediate consequences to fertility, potentially facilitating its detection. In contrast, drive in female meiosis could lead to very subtle skews in inheritance patterns and no overt signs of infertility; such driver alleles may therefore go unnoticed even if they are more pervasive [3, 4]. Surprisingly few meiotic drivers have been conclusively identified. There are three major roadblocks to the efficient identification and validation of meiotic drive alleles. First is the prevalent usage of isogenic, lab-domesticated organisms in genetics research; driver alleles can only be identified in heterozygotes. Second, the rapid evolution of suppressors to alleviate the deleterious effects of meiotic drivers can obscure drive in intraspecific crosses and also rapidly extinguish meiotic drive alleles by eliminating their evolutionary advantage [5]. Third, it can often be difficult to distinguish the actions of meiotic drive alleles from inherent viability defects associated with the underrepresented allele. Overcoming many of these limitations, a new report in this issue of PLOS Genetics by de Villena and colleagues provides compelling evidence for a massive copy number expansion that is causally linked to female meiotic drive in mice [6].

Didion et al. began their study by investigating the genetic basis of transmission ratio distortion (TRD) of a region of mouse chromosome 2. This TRD had been previously observed in a number of crosses, including, most recently, in the recombinant inbred Collaborative Cross (CC) lines designed for genetic analyses [7]. TRD was observed in favor of the WSB/EiJ allele across a ~50 Mb region in the middle of chromosome 2 (WSB/EiJ was one of the eight original inbred strains used to create recombinant inbred lines [5]). In the present report, Didion et al. first confirmed the TRD favoring the WSB/EiJ allele in the Diversity Outbred (DO) population of outbred mice derived from CC lines designed for mapping traits. Crosses of various WSB/EiJ heterozygotes to tester strains showed that TRD was specific to heterozygous females. These findings already eliminated the possibility of postmeiotic dysfunction of selective male gametes as the basis for TRD; such postmeiotic dysfunction is the basis for TRD caused by Segregation Distorter in Drosophila melanogaster [8] and the t-haplotype in mouse [9].

This finding left open two possibilities, the first being that the bias originates from a selective postfertilization defect. Indeed, Didion et al. find that the amount of TRD does negatively correlate with litter size, suggesting that lethality of oocytes or embryos inheriting the non-WSB/EiJ allele could contribute to the TRD. However, the observed decreases in litter sizes are insufficient to explain the magnitude of TRD observed. In addition, despite the decreased litter sizes, the absolute number of progeny inheriting the WSB/EiJ allele in crosses exhibiting TRD was significantly higher than the number inheriting the allele in crosses without TRD. Thus, although embryonic lethality occurs in crosses showing TRD, Didion et al. propose that “true meiotic drive” must occur to fully explain the TRD in favor of the WSB/EiJ allele. Such drive occurs because, in female meiosis, only one of four meiotic products is selected in the oocyte [10].

Based on the meiotic drive inference, the authors dubbed the causative allele R2d2 (Responder to drive on chr. 2). To map R2d2, Didion et al. again exploited the impressive genetic power of the CC and DO lines. By genotyping over 400 mice, they identified recombination events within the candidate region on chromosome 2. All eight recombinant chromosomes that showed TRD shared a 9.3 Mb region containing a 127 kb unit of DNA (R2d2) that is repeated ~36 times in WSB/EiJ (see Fig. 1). Three other mice strains previously shown to exhibit TRD also contain R2d sequences at a high copy number. In contrast, R2d is only present at 1–2X copy number in the reference mouse genome and other mouse strains that lack the TRD phenotype. In non-TRD strains, like the reference genome, the R2d sequences appear to be present only in the (presumed) ancestral location spread over a 158 kb region (R2d1) ~6 Mb distal from the R2d2 locus (Fig. 1A). These results suggested the hypothesis that whereas all mouse strains contain R2d1, a copy number expansion at R2d2 is causative for TRD (Fig. 1). A satisfying confirmation of the hypothesis emerged from the genetic instability of the R2d2 repeat cluster. Didion et al. identified a WSB/EiJ-derived female in which one of the R2d2 repeat arrays had collapsed from ~34 to ~11 copies. This collapse resulted in both the loss of TRD and increased litter sizes (Fig. 1). Together, this beautiful series of experiments, relying on both classical genetics and genomic assembly mapping, identify and confirm the R2d2 expansion as causal for female meiotic drive.

Transmission ratio distortion (TRD) caused by a high copy <i>R2d2</i> array.
Fig. 1. Transmission ratio distortion (TRD) caused by a high copy R2d2 array.
In the absence of the R2d2 sequence (e.g., C57BL/6J), or when the copy number of the sequence is low (e.g., CAST/EiJ), allele transmission through heterozygous females is Mendelian and no embryonic lethality is observed. When the copy number is high in the appropriate genetic background (e.g. WSB/EiJ), the litter sizes of heterozygous females are reduced and the R2d2 locus shows TRD. When the copy number of R2d2 repeats decreases (e.g., DO-G13–44), litter sizes increase and Mendelian segregation is restored.

How does R2d2 expansion cause meiotic drive? Didion et al. favor the model that the R2d2 expansion leads to preferential inheritance in meiosis. Such non-Mendelian inheritance would occur via the formation of neocentromere-like activity on cis-acting sequences expanded in the R2d2 cluster, which favor their preferential orientation and thereby inclusion in the oocyte [11]. This situation would be highly reminiscent of the knob elements in maize that also take advantage of female meiosis asymmetries to increase their likelihood of inclusion into the oocyte [12]. Akin to knob elements, R2d2 drive might occur in Meiosis II [3, 4]. Under this model, the ensuing R2d2-dependent embryonic lethality would result from generating eggs that are aneuploid for chromosome 2.

Not all dams heterozygous for R2d2 show TRD, implicating the requirement of at least one additional modifier locus that must be present to manifest TRD. These additional loci could represent allelic variants of meiotic drive suppressors, which reduce the harmful fitness effects of drive and associated embryonic lethality. Alternatively, these could represent “trans-acting factors” (distorters) that bind R2d2 and endow it with microtubule attachment or motor function that results in neocentromere activity [12]. These findings would be at odds with predictions from the theory that distorter and responder loci ought to be tightly linked, so as to not be separated by recombination [13]. One intriguing possibility is that the only protein encoded by the R2d unit, Cwc2, might itself bind the R2d2 cluster and contribute to TRD.

In sum, the paper by Didion et al. represents a tour de force in characterizing a complex multilocus TRD system in a genetically tractable mammalian model system. Uncovering the molecular mechanisms underlying the transmission of exceptional meiotic drive alleles like R2d2, as well as their evolutionary origins, will broaden our general understanding of fertility and chromosome segregation. Such work also reinforces previous findings about the insidious and relentless nature of meiotic competition between chromosomes [1, 2, 10].


1. Crow JF (1988) The ultraselfish gene. Genetics 118: 389–391. 3130288

2. Burt A, Trivers R (2006) Genes in conflict: the biology of selfish genetic elements. Cambridge, Mass.: Belknap Press of Harvard University Press. viii, 602 p., 608 p. of plates p.

3. Tao Y, Hartl DL, Laurie CC (2001) Sex-ratio segregation distortion associated with reproductive isolation in Drosophila. Proc Natl Acad Sci U S A 98: 13183–13188. doi: 10.1073/pnas.231478798 11687638

4. Didion JP, Morgan AP, Clayshulte AM-F, Mcmullan RC, Yadgary L, et al. (2014) A multi-megabase copy number gain causes maternal transmission ratio distortion on mouse Chromosome 2. PLOS Genet 11: e1004850.

5. Collaborative Cross C (2012) The genome architecture of the Collaborative Cross mouse genetic reference population. Genetics 190: 389–401. doi: 10.1534/genetics.111.132639

6. Larracuente AM, Presgraves DC (2012) The selfish Segregation Distorter gene complex of Drosophila melanogaster. Genetics 192: 33–53. doi: 10.1534/genetics.112.141390 22964836

7. Ardlie KG (1998) Putting the brake on drive: meiotic drive of t haplotypes in natural populations of mice. Trends Genet 14: 189–193. doi: 10.1016/S0168-9525(98)01455-3 9613203

8. Sandler L, Novitski E (1957) Meiotic Drive as an Evolutionary Force. The American Naturalist 91: 105–110. doi: 10.1086/281969

9. Zwick ME, Salstrom JL, Langley CH (1999) Genetic variation in rates of nondisjunction: association of two naturally occurring polymorphisms in the chromokinesin nod with increased rates of nondisjunction in Drosophila melanogaster. Genetics 152: 1605–1614. 10430586

10. Yu HG, Hiatt EN, Chan A, Sweeney M, Dawe RK (1997) Neocentromere-mediated chromosome movement in maize. J Cell Biol 139: 831–840. doi: 10.1083/jcb.139.4.831 9362502

11. Buckler ESt, Phelps-Durr TL, Buckler CS, Dawe RK, Doebley JF, et al. (1999) Meiotic drive of chromosomal knobs reshaped the maize genome. Genetics 153: 415–426. 10471723

12. Malik HS (2005) Mimulus finds centromeres in the driver’s seat. Trends Ecol Evol 20: 151–154. doi: 10.1016/j.tree.2005.01.014 16701359

13. Charlesworth B, Hartl DL (1978) Population Dynamics of the Segregation Distorter Polymorphism of DROSOPHILA MELANOGASTER. Genetics 89: 171–192. 17248828

Genetika Reprodukční medicína

Článek vyšel v časopise

PLOS Genetics

2015 Číslo 2

Nejčtenější v tomto čísle

Tomuto tématu se dále věnují…


Zvyšte si kvalifikaci online z pohodlí domova

Imunitní trombocytopenie (ITP) u dospělých pacientů
nový kurz
Autoři: prof. MUDr. Tomáš Kozák, Ph.D., MBA

Pěnová skleroterapie
Autoři: MUDr. Marek Šlais

White paper - jak vidíme optimální péči o zubní náhrady
Autoři: MUDr. Jindřich Charvát, CSc.

Hemofilie - série kurzů

Faktory ovlivňující léčbu levotyroxinem

Všechny kurzy
Kurzy Doporučená témata Časopisy
Zapomenuté heslo

Nemáte účet?  Registrujte se

Zapomenuté heslo

Zadejte e-mailovou adresu se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.


Nemáte účet?  Registrujte se

VIRTUÁLNÍ ČEKÁRNA ČR Jste praktický lékař nebo pediatr? Zapojte se! Jste praktik nebo pediatr? Zapojte se!