The canonical α-SNAP is essential for gametophytic development in Arabidopsis

English version

Autoři: Fei Liu ^aff001; Ji-Peng Li ^aff002; Lu-Shen Li ^aff002; Qi Liu ^aff002; Shan-Wei Li ^aff002; Ming-Lei Song ^aff002; Sha Li ^aff001; Yan Zhang ^aff002
Působiště autorů: Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin, China ^aff001; State Key laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, China ^aff002
Vyšlo v časopise: The canonical α-SNAP is essential for gametophytic development in Arabidopsis. PLoS Genet 17(4): e1009505. doi:10.1371/journal.pgen.1009505
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1009505

Souhrn

The development of male and female gametophytes is a pre-requisite for successful reproduction of angiosperms. Factors mediating vesicular trafficking are among the key regulators controlling gametophytic development. Fusion between vesicles and target membranes requires the assembly of a fusogenic soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs) complex, whose disassembly in turn ensures the recycle of individual SNARE components. The disassembly of post-fusion SNARE complexes is controlled by the AAA⁺ ATPase N-ethylmaleimide-sensitive factor (Sec18/NSF) and soluble NSF attachment protein (Sec17/α-SNAP) in yeast and metazoans. Although non-canonical α-SNAPs have been functionally characterized in soybeans, the biological function of canonical α-SNAPs has yet to be demonstrated in plants. We report here that the canonical α-SNAP in Arabidopsis is essential for male and female gametophytic development. Functional loss of the canonical α-SNAP in Arabidopsis results in gametophytic lethality by arresting the first mitosis during gametogenesis. We further show that Arabidopsis α-SNAP encodes two isoforms due to alternative splicing. Both isoforms interact with the Arabidopsis homolog of NSF whereas have distinct subcellular localizations. The presence of similar alternative splicing of human α-SNAP indicates that functional distinction of two α-SNAP isoforms is evolutionarily conserved.

Klíčová slova:

Anthers – Arabidopsis thaliana – Confocal laser microscopy – DAPI staining – Ovules – Plant genomics – Pollen – Embryo sac

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