Extracellular spreading of Wingless is required for Drosophila oogenesis


Autoři: Xiaoxi Wang aff001;  Kimberly S. LaFever aff001;  Indrayani Waghmare aff001;  Andrea Page-McCaw aff001
Působiště autorů: Department of Cell and Developmental Biology, Program in Developmental Biology, Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America aff001
Vyšlo v časopise: Extracellular spreading of Wingless is required for Drosophila oogenesis. PLoS Genet 17(4): e1009469. doi:10.1371/journal.pgen.1009469
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1009469

Souhrn

Recent studies have investigated whether the Wnt family of extracellular ligands can signal at long range, spreading from their source and acting as morphogens, or whether they signal only in a juxtacrine manner to neighboring cells. The original evidence for long-range Wnt signaling arose from studies of Wg, a Drosophila Wnt protein, which patterns the wing disc over several cell diameters from a central source of Wg ligand. However, the requirement of long-range Wg for patterning was called into question when it was reported that replacing the secreted protein Wg with a membrane-tethered version, NRT-Wg, results in flies with normally patterned wings. We and others previously reported that Wg spreads in the ovary about 50 μm or 5 cell diameters, from the cap cells to the follicle stem cells (FSCs) and that Wg stimulates FSC proliferation. We used the NRT-wg flies to analyze the consequence of tethering Wg to the cap cells. NRT-wg homozygous flies are sickly, but we found that hemizygous NRT-wg/null flies, carrying only one copy of tethered Wingless, were significantly healthier. Despite their overall improved health, these hemizygous flies displayed dramatic reductions in fertility and in FSC proliferation. Further, FSC proliferation was nearly undetectable when the wg locus was converted to NRT-wg only in adults, and the resulting germarium phenotype was consistent with a previously reported wg loss-of-function phenotype. We conclude that Wg protein spreads from its source cells in the germarium to promote FSC proliferation.

Klíčová slova:

Toxicity – DAPI staining – Eggs – Homozygosity – Ovaries – Wnt signaling cascade – Juxtacrine signaling – Bird eggs


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