Dynamic post-transcriptional regulation by Mrn1 links cell wall homeostasis to mitochondrial structure and function


Autoři: Kendra Reynaud aff001;  Molly Brothers aff002;  Michael Ly aff002;  Nicholas T. Ingolia aff001
Působiště autorů: California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, California, United States of America aff001;  Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, United States of America aff002
Vyšlo v časopise: Dynamic post-transcriptional regulation by Mrn1 links cell wall homeostasis to mitochondrial structure and function. PLoS Genet 17(4): e1009521. doi:10.1371/journal.pgen.1009521
Kategorie: Research Article
doi: 10.1371/journal.pgen.1009521

Souhrn

The RNA-binding protein Mrn1 in Saccharomyces cerevisiae targets over 300 messenger RNAs, including many involved in cell wall biogenesis. The impact of Mrn1 on these target transcripts is not known, however, nor is the cellular role for this regulation. We have shown that Mrn1 represses target mRNAs through the action of its disordered, asparagine-rich amino-terminus. Its endogenous targets include the paralogous SUN domain proteins Nca3 and Uth1, which affect mitochondrial and cell wall structure and function. While loss of MRN1 has no effect on fermentative growth, we found that mrn1Δ yeast adapt more quickly to respiratory conditions. These cells also have enlarged mitochondria in fermentative conditions, mediated in part by dysregulation of NCA3, and this may explain their faster switch to respiration. Our analyses indicated that Mrn1 acts as a hub for integrating cell wall integrity and mitochondrial biosynthesis in a carbon-source responsive manner.

Klíčová slova:

Mitochondria – Biosynthesis – Cell walls – Gene expression – Glucose – Membrane proteins – Messenger RNA – Yeast


Zdroje

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