Juvenile hormone suppresses aggregation behavior through influencing antennal gene expression in locusts

Autoři: Wei Guo aff001;  Juan Song aff001;  Pengcheng Yang aff003;  Xiangyong Chen aff001;  Dafeng Chen aff001;  Dani Ren aff001;  Le Kang aff001;  Xianhui Wang aff001
Působiště autorů: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China aff001;  CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, China aff002;  Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China aff003
Vyšlo v časopise: Juvenile hormone suppresses aggregation behavior through influencing antennal gene expression in locusts. PLoS Genet 16(4): e32767. doi:10.1371/journal.pgen.1008762
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008762


Animals often exhibit dramatically behavioral plasticity depending on their internal physiological state, yet little is known about the underlying molecular mechanisms. The migratory locust, Locusta migratoria, provides an excellent model for addressing these questions because of their famous phase polyphenism involving remarkably behavioral plasticity between gregarious and solitarious phases. Here, we report that a major insect hormone, juvenile hormone, is involved in the regulation of this behavioral plasticity related to phase change by influencing the expression levels of olfactory-related genes in the migratory locust. We found that the treatment of juvenile hormone analog, methoprene, can significantly shift the olfactory responses of gregarious nymphs from attraction to repulsion to the volatiles released by gregarious nymphs. In contrast, the repulsion behavior of solitarious nymphs significantly decreased when they were treated with precocene or injected with double-stranded RNA of JHAMT, a juvenile hormone acid O-methyltransferase. Further, JH receptor Met or JH-response gene Kr-h1 knockdown phenocopied the JH-deprivation effects on olfactory behavior. RNA-seq analysis identified 122 differentially expressed genes in antennae after methoprene application on gregarious nymphs. Interestingly, several olfactory-related genes were especially enriched, including takeout (TO) and chemosensory protein (CSP) which have key roles in behavioral phase change of locusts. Furthermore, methoprene application and Met or Kr-h1 knockdown resulted in simultaneous changes of both TO1 and CSP3 expression to reverse pattern, which mediated the transition between repulsion and attraction responses to gregarious volatiles. Our results suggest the regulatory roles of a pleiotropic hormone in locust behavioral plasticity through modulating gene expression in the peripheral olfactory system.

Klíčová slova:

Acetones – Animal antennae – Animal behavior – Decision making – Gene expression – Gene regulation – Locusts – Nymphs


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