Loss of Cdc13 causes genome instability by a deficiency in replication-dependent telomere capping

English version

Autoři: Rachel E. Langston ^aff001; Dominic Palazzola ^aff001; Erin Bonnell ^aff002; Raymund J. Wellinger ^aff002; Ted Weinert ^aff001
Působiště autorů: Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona, United States of America ^aff001; Department of Microbiology and Infectiology, Université de Sherbrooke, Sherbrooke, Quebec, Canada ^aff002
Vyšlo v časopise: Loss of Cdc13 causes genome instability by a deficiency in replication-dependent telomere capping. PLoS Genet 16(4): e32767. doi:10.1371/journal.pgen.1008733
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008733

Souhrn

In budding yeast, Cdc13, Stn1, and Ten1 form the telomere-binding heterotrimer CST complex. Here we investigate the role of Cdc13/CST in maintaining genome stability by using a Chr VII disome system that can generate recombinants, chromosome loss, and enigmatic unstable chromosomes. In cells expressing a temperature sensitive CDC13 allele, cdc13^F684S, unstable chromosomes frequently arise from problems in or near a telomere. We found that, when Cdc13 is defective, passage through S phase causes Exo1-dependent ssDNA and unstable chromosomes that are then the source for additional chromosome instability events (e.g. recombinants, chromosome truncations, dicentrics, and/or chromosome loss). We observed that genome instability arises from a defect in Cdc13’s function during DNA replication, not Cdc13’s putative post-replication telomere capping function. The molecular nature of the initial unstable chromosomes formed by a Cdc13-defect involves ssDNA and does not involve homologous recombination nor non-homologous end joining; we speculate the original unstable chromosome may be a one-ended double strand break. This system defines a link between Cdc13’s function during DNA replication and genome stability in the form of unstable chromosomes, that then progress to form other chromosome changes.

Klíčová slova:

Cell cycle and cell division – DNA recombination – DNA replication – Genetic networks – Chromosome structure and function – Protein structure networks – Synthesis phase – Telomeres

Zdroje

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