Spatiotemporal cytoskeleton organizations determine morphogenesis of multicellular trichomes in tomato

English version

Autoři: Jiang Chang ^aff001; Zhijing Xu ^aff001; Meng Li ^aff001; Meina Yang ^aff001; Haiyang Qin ^aff001; Jie Yang ^aff001; Shuang Wu ^aff001
Působiště autorů: College of Horticulture, FAFU-UCR Joint Center and Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, China ^aff001
Vyšlo v časopise: Spatiotemporal cytoskeleton organizations determine morphogenesis of multicellular trichomes in tomato. PLoS Genet 15(10): e1008438. doi:10.1371/journal.pgen.1008438
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008438

Souhrn

Plant trichomes originate from epidermal cell, forming protective structure from abiotic and biotic stresses. Different from the unicellular trichome in Arabidopsis, tomato trichomes are multicellular structure and can be classified into seven different types based on cell number, shape and the presence of glandular cells. Despite the importance of tomato trichomes in insect resistance, our understanding of the tomato trichome morphogenesis remains elusive. In this study, we quantitatively analyzed morphological traits of trichomes in tomato and further performed live imaging of cytoskeletons in stably transformed lines with actin and microtubule markers. At different developmental stages, two types of cytoskeletons exhibited distinct patterns in different trichome cells, ranging from transverse, spiral to longitudinal. This gradual transition of actin filament angle from basal to top cells could correlate with the spatial expansion mode in different cells. Further genetic screen for aberrant trichome morphology led to the discovery of a number of independent mutations in SCAR/WAVE and ARP2/3 complex, which resulted in actin bundling and distorted trichomes. Disruption of microtubules caused isotropic expansion while abolished actin filaments entirely inhibited axial extension of trichomes, indicating that microtubules and actin filaments may control distinct aspects of trichome cell expansion. Our results shed light on the roles of cytoskeletons in the formation of multicellular structure of tomato trichomes.

Klíčová slova:

Actins – Anisotropy – Microtubules – Tomatoes – Trichomes – Actin filaments – Cytoskeleton – Basal cells

Zdroje

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