Centromeric SMC1 promotes centromer clustering and stabilizes meiotic homolog pairing

Autoři: Talia Hatkevich aff001;  Vincent Boudreau aff002;  Thomas Rubin aff003;  Paul S. Maddox aff002;  Jean-René Huynh aff003;  Jeff Sekelsky aff001
Působiště autorů: Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America aff001;  Department of Biology, University of North Carolina, Chapel Hill, North Carolina, United States of America aff002;  CIRB, Collège de France, PSL Research University, CNRS UMR7241, Inserm U1050, Paris, France aff003;  Integrative Program in Biological and Genome Sciences, University of North Carolina, Chapel Hill, North Carolina, United States of America aff004
Vyšlo v časopise: Centromeric SMC1 promotes centromer clustering and stabilizes meiotic homolog pairing. PLoS Genet 15(10): e32767. doi:10.1371/journal.pgen.1008412
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008412


During meiosis, each chromosome must selectively pair and synapse with its own unique homolog to enable crossover formation and subsequent segregation. How homolog pairing is maintained in early meiosis to ensure synapsis occurs exclusively between homologs is unknown. We aimed to further understand this process by examining the meiotic defects of a unique Drosophila mutant, Mcm5A7. We found that Mcm5A7 mutants are proficient in homolog pairing at meiotic onset yet fail to maintain pairing as meiotic synapsis ensues, causing seemingly normal synapsis between non-homologous loci. This pairing defect corresponds with a reduction of SMC1-dependent centromere clustering at meiotic onset. Overexpressing SMC1 in this mutant significantly restores centromere clustering, homolog pairing, and crossover formation. These data indicate that the initial meiotic pairing of homologs is not sufficient to yield synapsis exclusively between homologs and provide a model in which meiotic homolog pairing must be stabilized by centromeric SMC1 to ensure proper synapsis.

Klíčová slova:

Centromeres – Drosophila melanogaster – Chromosome pairs – Meiosis – Ovaries – Homologous chromosomes – Synapsis – Corolla


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