An EHBP-1-SID-3-DYN-1 axis promotes membranous tubule fission during endocytic recycling

English version

Autoři: Jinghu Gao ^aff001; Linyue Zhao ^aff001; Qian Luo ^aff001; Shuyao Liu ^aff001; Ziyang Lin ^aff001; Peixiang Wang ^aff001; Xin Fu ^aff001; Juan Chen ^aff001; Hongjie Zhang ^aff002; Long Lin ^aff001; Anbing Shi ^aff001
Působiště autorů: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China ^aff001; Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau SAR, China ^aff002; Institute for Brain Research, Huazhong University of Science and Technology, Wuhan, Hubei, China ^aff003; Key Laboratory of Neurological Disease of National Education Ministry, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China ^aff004
Vyšlo v časopise: An EHBP-1-SID-3-DYN-1 axis promotes membranous tubule fission during endocytic recycling. PLoS Genet 16(5): e32767. doi:10.1371/journal.pgen.1008763
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008763

Souhrn

The ACK family tyrosine kinase SID-3 is involved in the endocytic uptake of double-stranded RNA. Here we identified SID-3 as a previously unappreciated recycling regulator in the Caenorhabditis elegans intestine. The RAB-10 effector EHBP-1 is required for the endosomal localization of SID-3. Accordingly, animals with loss of SID-3 phenocopied the recycling defects observed in ehbp-1 and rab-10 single mutants. Moreover, we detected sequential protein interactions between EHBP-1, SID-3, NCK-1, and DYN-1. In the absence of SID-3, DYN-1 failed to localize at tubular recycling endosomes, and membrane tubules breaking away from endosomes were mostly absent, suggesting that SID-3 acts synergistically with the downstream DYN-1 to promote endosomal tubule fission. In agreement with these observations, overexpression of DYN-1 significantly increased recycling transport in SID-3-deficient cells. Finally, we noticed that loss of RAB-10 or EHBP-1 compromised feeding RNAi efficiency in multiple tissues, implicating basolateral recycling in the transport of RNA silencing signals. Taken together, our study demonstrated that in C. elegans intestinal epithelia, SID-3 acts downstream of EHBP-1 to direct fission of recycling endosomal tubules in concert with NCK-1 and DYN-1.

Klíčová slova:

Caenorhabditis elegans – Cell membranes – Endosomes – Fluorescence imaging – Gastrointestinal tract – RNA interference – Tyrosine kinases – Vacuoles

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