Diversified regulation of circadian clock gene expression following whole genome duplication

Autoři: Alexander C. West aff001;  Marianne Iversen aff001;  Even H. Jørgensen aff001;  Simen R. Sandve aff002;  David G. Hazlerigg aff001;  Shona H. Wood aff001
Působiště autorů: Arctic chronobiology and physiology research group, Department of Arctic and Marine Biology, UiT – The Arctic University of Norway, Tromsø, Norway aff001;  Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences (IHA), Faculty of Life Sciences (BIOVIT), Norwegian University of Life Sciences (NMBU), Ås, Norway aff002
Vyšlo v časopise: Diversified regulation of circadian clock gene expression following whole genome duplication. PLoS Genet 16(10): e32767. doi:10.1371/journal.pgen.1009097
Kategorie: Research Article
doi: 10.1371/journal.pgen.1009097


Across taxa, circadian control of physiology and behavior arises from cell-autonomous oscillations in gene expression, governed by a networks of so-called ‘clock genes’, collectively forming transcription-translation feedback loops. In modern vertebrates, these networks contain multiple copies of clock gene family members, which arose through whole genome duplication (WGD) events during evolutionary history. It remains unclear to what extent multiple copies of clock gene family members are functionally redundant or have allowed for functional diversification. We addressed this problem through an analysis of clock gene expression in the Atlantic salmon, a representative of the salmonids, a group which has undergone at least 4 rounds of WGD since the base of the vertebrate lineage, giving an unusually large complement of clock genes. By comparing expression patterns across multiple tissues, and during development, we present evidence for gene- and tissue-specific divergence in expression patterns, consistent with functional diversification of clock gene duplicates. In contrast to mammals, we found no evidence for coupling between cortisol and circadian gene expression, but cortisol mediated non-circadian regulated expression of a subset of clock genes in the salmon gill was evident. This regulation is linked to changes in gill function necessary for the transition from fresh- to sea-water in anadromous fish. Overall, this analysis emphasises the potential for a richly diversified clock gene network to serve a mixture of circadian and non-circadian functions in vertebrate groups with complex genomes.

Klíčová slova:

Circadian oscillators – Circadian rhythms – Cortisol – Fish – Gene expression – Gene regulation – Gills – Fish genomics


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PLOS Genetics

2020 Číslo 10

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