Silencing of transcription factor encoding gene StTCP23 by small RNAs derived from the virulence modulating region of potato spindle tuber viroid is associated with symptom development in potato

Autoři: Sarina Bao aff001;  Robert A. Owens aff002;  Qinghua Sun aff001;  Hui Song aff001;  Yanan Liu aff001;  Andrew Leigh Eamens aff003;  Hao Feng aff001;  Hongzhi Tian aff001;  Ming-Bo Wang aff004;  Ruofang Zhang aff001
Působiště autorů: School of Life Sciences, Inner Mongolia University, Hohhot, China aff001;  Molecular Plant Pathology Laboratory, USDA/ARS, Beltsville, Maryland, United States of America aff002;  Centre for Plant Science, School of Environmental and Life Sciences, Faculty of Science, University of Newcastle, Australia aff003;  CSIRO Plant Industry, Canberra, Australia aff004
Vyšlo v časopise: Silencing of transcription factor encoding gene StTCP23 by small RNAs derived from the virulence modulating region of potato spindle tuber viroid is associated with symptom development in potato. PLoS Pathog 15(12): e32767. doi:10.1371/journal.ppat.1008110
Kategorie: Research Article
doi: 10.1371/journal.ppat.1008110


Viroids are small, non-protein-coding RNAs which can induce disease symptoms in a variety of plant species. Potato (Solanum tuberosum L.) is the natural host of Potato spindle tuber viroid (PSTVd) where infection results in stunting, distortion of leaves and tubers and yield loss. Replication of PSTVd is accompanied by the accumulation of viroid-derived small RNAs (sRNAs) proposed to play a central role in disease symptom development. Here we report that PSTVd sRNAs direct RNA silencing in potato against StTCP23, a member of the TCP (teosinte branched1/Cycloidea/Proliferating cell factor) transcription factor family genes that play an important role in plant growth and development as well as hormonal regulation, especially in responses to gibberellic acid (GA). The StTCP23 transcript has 21-nucleotide sequence complementarity in its 3ʹ untranslated region with the virulence-modulating region (VMR) of PSTVd strain RG1, and was downregulated in PSTVd-infected potato plants. Analysis using 3ʹ RNA ligase-mediated rapid amplification of cDNA ends (3ʹ RLM RACE) confirmed cleavage of StTCP23 transcript at the expected sites within the complementarity with VMR-derived sRNAs. Expression of these VMR sRNA sequences as artificial miRNAs (amiRNAs) in transgenic potato plants resulted in phenotypes reminiscent of PSTVd-RG1-infected plants. Furthermore, the severity of the phenotypes displayed was correlated with the level of amiRNA accumulation and the degree of amiRNA-directed down-regulation of StTCP23. In addition, virus-induced gene silencing (VIGS) of StTCP23 in potato also resulted in PSTVd-like phenotypes. Consistent with the function of TCP family genes, amiRNA lines in which StTCP23 expression was silenced showed a decrease in GA levels as well as alterations to the expression of GA biosynthesis and signaling genes previously implicated in tuber development. Application of GA to the amiRNA plants minimized the PSTVd-like phenotypes. Taken together, our results indicate that sRNAs derived from the VMR of PSTVd-RG1 direct silencing of StTCP23 expression, thereby disrupting the signaling pathways regulating GA metabolism and leading to plant stunting and formation of small and spindle-shaped tubers.

Klíčová slova:

Genetically modified plants – Gibberellins – Leaves – Phenotypes – Potato – Small interfering RNAs – Transcription factors – Tubers


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