Intra-host growth kinetics of dengue virus in the mosquito Aedes aegypti

Autoři: Mario Novelo aff001;  Matthew D. Hall aff001;  Damie Pak aff003;  Paul R. Young aff004;  Edward C. Holmes aff005;  Elizabeth A. McGraw aff001
Působiště autorů: School of Biological Sciences, Monash University, Melbourne, Victoria, Australia aff001;  Center for Infectious Disease Dynamics, Department of Entomology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States of America aff002;  Center for Infectious Disease Dynamics, Department of Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States of America aff003;  Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland, Australia aff004;  Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, New South Wales, Australia aff005
Vyšlo v časopise: Intra-host growth kinetics of dengue virus in the mosquito Aedes aegypti. PLoS Pathog 15(12): e32767. doi:10.1371/journal.ppat.1008218
Kategorie: Research Article
doi: 10.1371/journal.ppat.1008218


Dengue virus (DENV) transmission by mosquitoes is a time-dependent process that begins with the consumption of an infectious blood-meal. DENV infection then proceeds stepwise through the mosquito from the midgut to the carcass, and ultimately to the salivary glands, where it is secreted into saliva and then transmitted anew on a subsequent bite. We examined viral kinetics in tissues of the Aedes aegypti mosquito over a finely graded time course, and as per previous studies, found that initial viral dose and serotype strain diversity control infectivity. We also found that a threshold level of virus is required to establish body-wide infections and that replication kinetics in the early and intermediate tissues do not predict those of the salivary glands. Our findings have implications for mosquito GMO design, modeling the contribution of transmission to vector competence and the role of mosquito kinetics in the overall DENV epidemiological landscape.

Klíčová slova:

Aedes aegypti – Blood – Dengue virus – Dose prediction methods – Mosquitoes – Salivary glands – Viral load – Viral replication


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