Control of mRNA translation by dynamic ribosome modification
Autoři:
Lucia Grenga aff001; Richard Howard Little aff001; Govind Chandra aff001; Stuart Daniel Woodcock aff001; Gerhard Saalbach aff001; Richard James Morris aff003; Jacob George Malone aff001
Působiště autorů:
Molecular Microbiology, John Innes Centre, Norwich, Norfolk, United Kingdom
aff001; School of Biological Sciences, University of East Anglia, Norwich, Norfolk, United Kingdom
aff002; Computational and Systems Biology, John Innes Centre, Norwich, Norfolk, United Kingdom
aff003
Vyšlo v časopise:
Control of mRNA translation by dynamic ribosome modification. PLoS Genet 16(6): e32767. doi:10.1371/journal.pgen.1008837
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1008837
Souhrn
Control of mRNA translation is a crucial regulatory mechanism used by bacteria to respond to their environment. In the soil bacterium Pseudomonas fluorescens, RimK modifies the C-terminus of ribosomal protein RpsF to influence important aspects of rhizosphere colonisation through proteome remodelling. In this study, we show that RimK activity is itself under complex, multifactorial control by the co-transcribed phosphodiesterase trigger enzyme (RimA) and a polyglutamate-specific protease (RimB). Furthermore, biochemical experimentation and mathematical modelling reveal a role for the nucleotide second messenger cyclic-di-GMP in coordinating these activities. Active ribosome regulation by RimK occurs by two main routes: indirectly, through changes in the abundance of the global translational regulator Hfq and directly, with translation of surface attachment factors, amino acid transporters and key secreted molecules linked specifically to RpsF modification. Our findings show that post-translational ribosomal modification functions as a rapid-response mechanism that tunes global gene translation in response to environmental signals.
Klíčová slova:
Adenosine triphosphatase – Glutamate – Ligases – Messenger RNA – Proteases – Protein translation – Proteomes – Ribosomes
Zdroje
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