Regulation of epithelial integrity and organ growth by Tctp and Coracle in Drosophila

English version

Autoři: Sung-Ryeong Lee ^aff001; Sung-Tae Hong ^aff002; Kwang-Wook Choi ^aff001
Působiště autorů: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea ^aff001; Department of Biological Sciences, Korean Advanced Institute of Science and Technology, Daejeon, Republic of Korea ^aff001; Department of Anatomy & Cell Biology, Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, Republic of Korea ^aff002
Vyšlo v časopise: Regulation of epithelial integrity and organ growth by Tctp and Coracle in Drosophila. PLoS Genet 16(6): e32767. doi:10.1371/journal.pgen.1008885
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008885

Souhrn

Regulation of cell junctions is crucial for the integrity of epithelial tissues and organs. Cell junctions also play roles in controlling cell proliferation for organ growth. Translationally controlled tumor protein (TCTP) is a conserved protein involved in growth control, but its role in cell junctions is unknown. Here we show that Drosophila Tctp directly interacts with the septate junction protein Coracle (Cora) to regulate epithelial integrity and organ growth. Tctp localizes together with Cora in the epidermis of the embryo. Loss of Cora reduces the level of Tctp in the epidermis but not vice versa. cora/+ or Tctp/+ single heterozygotes develop normally to adulthood. However, double heterozygotes for cora and Tctp mutations show severe disruption of epithelia causing synthetic lethality in the embryo. Double knockdown of Cora and Tctp in eye imaginal disc synergistically leads to disruption of the eye disc, resulting in a severe reduction or loss of eye and head. Conversely, double knockdown of Cora and Tctp in wing disc causes overgrowth as well as cell death. Inhibition of cell death under this condition causes hyperplastic growth of the wing disc. Tctp also shows direct and functional interaction with Cora-associated factors like Yurt and Na⁺/K⁺-ATPase. This study suggests that proper levels of Tctp and Cora are essential for the maintenance of the Cora complex and the integrity of epithelia. Our data also provide evidence that both Cora and Tctp are required to suppress overgrowth in developing wing.

Klíčová slova:

Cell membranes – DAPI staining – Drosophila melanogaster – Embryos – Eyes – Genetic interactions – RNA interference – Septate junctions

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