TaTLP1 interacts with TaPR1 to contribute to wheat defense responses to leaf rust fungus

English version

Autoři: Fei Wang ^aff001; Shitao Yuan ^aff001; Wenyue Wu ^aff001; Yiqing Yang ^aff001; Zhongchi Cui ^aff001; Haiyan Wang ^aff001; Daqun Liu ^aff001
Působiště autorů: Center of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Hebei Agricultural University, Baoding, China ^aff001; Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China ^aff002
Vyšlo v časopise: TaTLP1 interacts with TaPR1 to contribute to wheat defense responses to leaf rust fungus. PLoS Genet 16(7): e32767. doi:10.1371/journal.pgen.1008713
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008713

Souhrn

Thaumatin-like proteins (TLPs), which are defined as pathogenesis-related protein family 5 (PR5) members, are common plant proteins involved in defense responses and confer antifungal activity against many plant pathogens. Our earlier studies have reported that the TaTLP1 gene was isolated from wheat and proved to be involved in wheat defense in response to leaf rust attack. The present study aims to identify the interacting proteins of TaTLP1 and characterize the role of the interaction between wheat and Puccinia triticina (Pt). Pull-down experiments designed to isolate the molecular target of TaTLP1 in tobacco resulted in the identification of TaPR1, a pathogenesis-related protein of family 1, and the interaction between TaTLP1 and TaPR1 was confirmed by yeast two-hybrid experiments (Y2H), bimolecular fluorescence complementation (BiFC), and co-immunoprecipitation (Co-IP). In vitro, TaTLP1 and TaPR1 together increased antifungal activity against Pt. In vivo, the disease resistance phenotype, histological observations of fungal growth and host responses, and accumulation of H₂O₂ in TaTLP1-TaPR1 in co-silenced plants indicated that co-silencing significantly enhanced wheat susceptibility compared to single knockdown TaTLP1 or TaPR1 plants. The accumulation of reactive oxygen species (ROS) was significantly reduced in co-silenced plants compared to controls during Pt infection, which suggested that the TaTLP1-TaPR1 interaction positively modulates wheat resistance to Pt in an ROS-dependent manner. Our findings provide new insights for understanding the roles of two different PRs, TaTLP1 and TaPR1, in wheat resistance to leaf rust.

Klíčová slova:

Antifungals – Antimicrobial resistance – Cysteine – Leaves – Plant defenses – Plant pathogens – Protein interactions – Wheat

Zdroje

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