Advances and challenges in genetic technologies to produce single-sex litters

Autoři: Charlotte Douglas aff001;  James M. A. Turner aff001
Působiště autorů: Sex Chromosome Biology Laboratory, The Francis Crick Institute, London, United Kingdom aff001
Vyšlo v časopise: Advances and challenges in genetic technologies to produce single-sex litters. PLoS Genet 16(7): e32767. doi:10.1371/journal.pgen.1008898
Kategorie: Review
doi: 10.1371/journal.pgen.1008898


There is currently a requirement for single-sex litters for many applications, including agriculture, pest control, and reducing animal culling in line with the 3Rs principles: Reduction, Replacement, and Refinement. The advent of CRISPR/Cas9 genome editing presents a new opportunity with which to potentially generate all-female or all-male litters. We review some of the historical nongenetic strategies employed to generate single-sex litters and investigate how genetic and genome editing techniques are currently being used to produce all-male or all-female progeny. Lastly, we speculate on future technologies for generating single-sex litters and the possible associated challenges.

Klíčová slova:

Embryos – Genetic engineering – Genetic loci – Pest control – Sperm – X chromosomes – Y chromosomes – Y-linked traits


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