Kombinovaný bioinformatický a literární přístup k identifikaci dlouhých nekódujících molekul RNA, které modulují signalizaci přes receptor vitaminu D u karcinomu prsu

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Autoři: Kholghi Oskooei Vahid; Ghafouri-Fard Soudeh; Omrani Mir Davood
Působiště autorů: Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Vyšlo v časopise: Klin Onkol 2018; 31(4): 264-269
Kategorie: Přehled
doi: https://doi.org/10.14735/amko2018264

Souhrn

Úvod:
Bylo prokázáno, že dlouhé nekódující RNA (lncRNA) jako důležitá frakce lidského transkriptomu hrají zásadní roli při regulaci signálních drah, které se podílejí na karcinogenezi. Mezi nimi je signalizace receptoru vitaminu D (VDR), jejíž účast na různých nádorech vč. nádoru prsu (breast cancer –⁠ BC) je patrná. Navzdory přítomnosti několika důkazů účasti lncRNA, stejně jako signalizace VDR v patogenezi BC, žádná souhrnná studie nehodnotila vztah mezi dysregulací lncRNA a signalizací VDR u BC.

Cíl:
Zavést bioinformatický přístup k identifikaci lncRNA, které modulují signalizaci VDR u BC. Tento přístup zahrnuje koexpresní analýzu, in silico identifikaci lncRNAs, které jsou zaměřeny na VDR a literární vyhledávání. Závěr: Předpokládá se, že desítky lncRNA ovlivní signalizaci VDR. Mezi nimi jsou některé lncRNA, jako je MALAT1, který má významnou roli v patogenezi BC. Identifikace lncRNA, které ovlivňují expresi genu VDR, je možná pomocí in silico analýzy. Vzhledem k prominentní roli VDR v patogenezi BC a dostupnosti modulačních činidel VDR je hodnocení VDR signalizační dráhy a souvisejících sítí praktického významu a nástroje bioinformatiky by měly usnadnit tuto činnost.

Klíčová slova:
receptor vitaminu D –⁠ dlouhé nekódující molekuly RNA –⁠ koexprese –⁠ bioinformatika –⁠ receptor kalcitriolu –⁠ výpočetní biologie

Tento článek je výňatkem z práce Vahida Kholghi Oskooei z lékařské fakulty, Univerzity Shahid Beheshti (registrační číslo 46).

Autoři deklarují, že v souvislosti s předmětem studie nemají žádné komerční zájmy.

Redakční rada potvrzuje, že rukopis práce splnil ICMJE kritéria pro publikace zasílané do biomedicínských časopisů.

Obdrženo: 17. 3. 2018

Accepted: 6. 5. 2018

Zdroje

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