RNA Binding Protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer


Autoři: Mrinmoyee Majumder aff001;  Viswanathan Palanisamy aff001
Působiště autorů: Department of Biochemistry and Molecular Biology, College of Medicine, Medical University of South Carolina, Charleston, SC, United States of America aff001
Vyšlo v časopise: RNA Binding Protein FXR1-miR301a-3p axis contributes to p21WAF1 degradation in oral cancer. PLoS Genet 16(1): e32767. doi:10.1371/journal.pgen.1008580
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008580

Souhrn

RNA-binding proteins (RBPs) associate with the primary, precursor, and mature microRNAs, which in turn control post-transcriptional gene regulation. Here, by small RNAseq, we show that RBP FXR1 controls the expression of a subset of mature miRNAs, including highly expressed miR301a-3p in oral cancer cells. We also confirm that FXR1 controls the stability of miR301a-3p. Exoribonuclease PNPT1 degrades miR301a-3p in the absence of FXR1 in oral cancer cells, and the degradation is rescued in the FXR1 and PNPT1 co-knockdown cells. In vitro, we show that PNPT1 is unable to bind and degrade the miRNA once the FXR1-miRNA complex forms. Both miR301a-3p and FXR1 cooperatively target the 3'-UTR of p21 mRNA to promote its degradation. Thus, our work illustrates the unique role of FXR1 that is critical for the stability of a subset of mature miRNAs or at least miR301a-3p to target p21 in oral cancer.

Klíčová slova:

Actins – Cancer treatment – Head and neck squamous cell carcinoma – Luciferase – Messenger RNA – MicroRNAs – Recombinant proteins – RNA-binding proteins


Zdroje

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