Systems genomics approaches provide new insights into Arabidopsis thaliana root growth regulation under combinatorial mineral nutrient limitation

Autoři: Nadia Bouain aff001;  Arthur Korte aff002;  Santosh B. Satbhai aff003;  Hye-In Nam aff005;  Seung Y. Rhee aff005;  Wolfgang Busch aff003;  Hatem Rouached aff001
Působiště autorů: BPMP, Univ Montpellier, CNRS, INRA, SupAgro, Montpellier, France aff001;  Evolutionary Genomics, Center for Computational and Theoretical Biology (CCTB), University Würzburg, Würzburg, Germany aff002;  Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Vienna, Austria aff003;  Plant Molecular and Cellular Biology Laboratory, and Integrative Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, United States of America aff004;  Department of Plant Biology, Carnegie Institution for Science, Stanford, California, United States of America aff005
Vyšlo v časopise: Systems genomics approaches provide new insights into Arabidopsis thaliana root growth regulation under combinatorial mineral nutrient limitation. PLoS Genet 15(11): e32767. doi:10.1371/journal.pgen.1008392
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008392


The molecular mechanisms by which plants modulate their root growth rate (RGR) in response to nutrient deficiency are largely unknown. Using Arabidopsis thaliana accessions, we analyzed RGR variation under combinatorial mineral nutrient deficiencies involving phosphorus (P), iron (Fe), and zinc (Zn). While -P stimulated early RGR of most accessions, -Fe or -Zn reduced it. The combination of either -P-Fe or -P-Zn led to suppression of the growth inhibition exerted by -Fe or -Zn alone. Surprisingly, root growth responses of the reference accession Columbia (Col-0) were not representative of the species under -P nor -Zn. Using a systems approach that combines GWAS, network-based candidate identification, and reverse genetic screen, we identified new genes that regulate root growth in -P-Fe: VIM1, FH6, and VDAC3. Our findings provide a framework to systematically identifying favorable allelic variations to improve root growth, and to better understand how plants sense and respond to multiple environmental cues.

Klíčová slova:

Arabidopsis thaliana – Gene regulation – Genome-wide association studies – Micronutrient deficiencies – Nutrients – Root growth – Seedlings – Iron deficiency


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