The genetic architecture of helminth-specific immune responses in a wild population of Soay sheep (Ovis aries)

Autoři: Alexandra M. Sparks aff001;  Kathryn Watt aff001;  Rona Sinclair aff001;  Jill G. Pilkington aff001;  Josephine M. Pemberton aff001;  Tom N. McNeilly aff003;  Daniel H. Nussey aff001;  Susan E. Johnston aff001
Působiště autorů: Institutes of Evolutionary Biology and Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom aff001;  Faculty of Biological Sciences, School of Biology, University of Leeds, Leeds, United Kingdom aff002;  Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, United Kingdom aff003
Vyšlo v časopise: The genetic architecture of helminth-specific immune responses in a wild population of Soay sheep (Ovis aries). PLoS Genet 15(11): e32767. doi:10.1371/journal.pgen.1008461
Kategorie: Research Article
doi: 10.1371/journal.pgen.1008461


Much of our knowledge of the drivers of immune variation, and how these responses vary over time, comes from humans, domesticated livestock or laboratory organisms. While the genetic basis of variation in immune responses have been investigated in these systems, there is a poor understanding of how genetic variation influences immunity in natural, untreated populations living in complex environments. Here, we examine the genetic architecture of variation in immune traits in the Soay sheep of St Kilda, an unmanaged population of sheep infected with strongyle gastrointestinal nematodes. We assayed IgA, IgE and IgG antibodies against the prevalent nematode Teladorsagia circumcincta in the blood plasma of > 3,000 sheep collected over 26 years. Antibody levels were significantly heritable (h2 = 0.21 to 0.57) and highly stable over an individual’s lifespan. IgA levels were strongly associated with a region on chromosome 24 explaining 21.1% and 24.5% of heritable variation in lambs and adults, respectively. This region was adjacent to two candidate loci, Class II Major Histocompatibility Complex Transactivator (CIITA) and C-Type Lectin Domain Containing 16A (CLEC16A). Lamb IgA levels were also associated with the immunoglobulin heavy constant loci (IGH) complex, and adult IgE levels and lamb IgA and IgG levels were associated with the major histocompatibility complex (MHC). This study provides evidence of high heritability of a complex immunological trait under natural conditions and provides the first evidence from a genome-wide study that large effect genes located outside the MHC region exist for immune traits in the wild.

Klíčová slova:

Antibodies – Genetic loci – Genetic polymorphism – Genome-wide association studies – Major histocompatibility complex – Molecular genetics – Sheep – Genetics of the immune system


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2019 Číslo 11

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