#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Precision Cancer Diagnostics: Tracking Genomic Evolution in Clinical Trials


In a Perspective, Francisco Beca and Andrew Beck discuss Charles Swanton and colleagues' accompanying Research Article on somatic mutations in patients with inflammatory breast cancer treated in a Phase II clinical trial.


Published in the journal: . PLoS Med 13(12): e32767. doi:10.1371/journal.pmed.1002177
Category: Perspective
doi: https://doi.org/10.1371/journal.pmed.1002177

Summary

In a Perspective, Francisco Beca and Andrew Beck discuss Charles Swanton and colleagues' accompanying Research Article on somatic mutations in patients with inflammatory breast cancer treated in a Phase II clinical trial.

It has been more than 15 years since Time magazine published a cover story highlighting the success of the targeted drug imatinib in the fight against chronic myeloid leukemia [1]. At the time, we were still two years away from completion of the first human genome sequence as part of the Human Genome Project, and the cost of sequencing an entire human genome was estimated to be around US$100 million [2]. Fifteen years later, sequencing technologies have evolved tremendously and have driven down the cost of sequencing ~100,000-fold. Today, genomic profiling of patients’ tumors using next-generation sequencing is readily available from a variety of academic institutions and commercial vendors. The idea of using genomic sequencing data in cancer diagnostics is now more appealing than ever, and there is great hope in the community that the increasing availability of genomic data will result in more success stories for precision cancer medicine, in which knowledge of the genetic mechanism of disease will lead to development of more active therapies and improved patient outcomes.

Breast cancer has been a major focus of research efforts in precision oncology. For decades, treatment allocation to hormonal therapy has been based on biomarker profiles. Breast cancer is one of the first solid malignancies in which a defined genomic event with clear clinical and pathological implications, human epidermal growth factor receptor 2 (HER2) amplification, was found and for which a targeted agent that clearly modifies the natural history of the disease was made readily available [3]. Due in part to these successes, breast cancer survivorship is presently measured in intervals of five or more years, rather than in single years or months. But that is not the case for all breast cancer types. Inflammatory breast cancer (IBC) is a particularly aggressive form of breast cancer that tends to affect younger women. IBC is frequently HER2-overexpressing, and the disease’s hallmark is the clinical inflammatory symptoms on the breast skin due to numerous dermal lymphatic emboli. Like in other cancer types, genomic profiling has led to identification of multiple genes that are involved in IBC [4,5]. Assessments of molecular features and quantitative measures of intratumor heterogeneity are now in the translation phase from the research setting, and their clinical validity is being tested [6,7].

In the accompanying research article now published in PLOS Medicine, Charles Swanton and coworkers report the results of an open-label, Phase II trial of afatinib, a tyrosine kinase inhibitor selective for ErbB family receptors, for the treatment of HER2-positive IBC [8]. This is one of the first clinical trials to prospectively integrate longitudinal whole exome sequencing (WES) in a trial for drug development and to our knowledge the first reported in IBC. Swanton and colleagues’ trial had a small final sample size and shortened trial duration because of the reporting of the results of the LUX-Breast 1 trial, in which an afatinib-containing regimen was associated with shorter overall survival and was less tolerable than a regimen containing trastuzumab in HER2-positive metastatic breast cancer. But, despite this, the investigators were able to show clinical responses with afatinib, with or without vinorelbine, in trastuzumab-naïve HER-2 positive IBC. Additionally, in nine patients it was possible to compare the clonal architecture of the tumors before and after treatment because of the planned exome sequencing at two distinct time points. In the majority of patients for whom it was possible to compare the clonal architecture of the tumors before and after treatment, the clonal composition remained largely the same between the two time points, without definitive evidence of an evolutionary bottleneck. As the authors posit, several factors could explain this. Perhaps the simplest explanation is that, since in eight of these patients there was no clinical benefit from therapy, there may not have been enough selective pressure to drive tumor evolution—in contrast to the mechanism by which ESR1 mutations emerge in breast cancer during the course of endocrine treatment [9].

Although most patients in Swanton and colleagues’ study did not show definitive evidence of clonal evolution or benefit from therapy, there was a significant clonal shift in one of the patients. This important observation was only possible because of the prospectively planned WES analysis, demonstrating the ability of prospectively planned genomic profiling within the setting of a clinical trial to improve knowledge about IBC in individual patients, even when the study’s overall sample size is small and the overall therapeutic benefit of the experimental treatment is not established. We expect that future clinical trials in oncology will increasingly incorporate prospective deep genomic tumor characterization into their study designs, resulting in new biological insights for individual cancer patients regarding the causes of their disease and its progression over time.

For a disease as complex as inflammatory breast cancer, a success story resembling imatinib for chronic myelogenous leukemia is not likely to be realized; we may never identify a critical, single genomic driver and therapeutic vulnerability that is common across most IBC patients. However, it is likely that with increasing sequencing of individual tumors within the course of treatment, we will gain a deeper understanding of how IBC responds and progresses in individual patients, which may lead to better and safer therapies tailored to the dynamic genomic evolution of an individual patient’s disease.


Zdroje

1. Lemonick MD, Park A. New Hope For Cancer. Time Magazine. 2001 May. http://content.time.com/time/covers/0,16641,20010528,00.html

2. National Human Genome Research Institute. The Cost of Sequencing a Human Genome. National Human Genome Research Institute website. [cited 2016 Jan 1]. https://www.genome.gov/sequencingcosts/

3. Moasser MM, Krop IE. The Evolving Landscape of HER2 Targeting in Breast Cancer. JAMA Oncol. 2015 Nov;1(8):1154–61. http://www.ncbi.nlm.nih.gov/pubmed/26204261 doi: 10.1001/jamaoncol.2015.2286 26204261

4. Ross JS, Ali SM, Wang K, Khaira D, Palma NA, Chmielecki J, et al. Comprehensive genomic profiling of inflammatory breast cancer cases reveals a high frequency of clinically relevant genomic alterations. Breast Cancer Res Treat. 2015 Nov;154(1):155–62. http://www.ncbi.nlm.nih.gov/pubmed/26458824 doi: 10.1007/s10549-015-3592-z 26458824

5. Iwamoto T, Bianchini G, Qi Y, Cristofanilli M, Lucci A, Woodward WA, et al. Different gene expressions are associated with the different molecular subtypes of inflammatory breast cancer. Breast Cancer Res Treat. 2011 Feb;125(3):785–95. http://www.ncbi.nlm.nih.gov/pubmed/21153052 doi: 10.1007/s10549-010-1280-6 21153052

6. Parker JS, Perou CM. Tumor Heterogeneity: Focus on the Leaves, the Trees, or the Forest? Cancer Cell. 2015 Aug 10;28(2):149–50. http://www.ncbi.nlm.nih.gov/pubmed/26267531 doi: 10.1016/j.ccell.2015.07.011 26267531

7. Beca F, Polyak K. Intratumor Heterogeneity in Breast Cancer. Adv Exp Med Biol. 2016;882:169–89. http://www.ncbi.nlm.nih.gov/pubmed/26987535 doi: 10.1007/978-3-319-22909-6_7 26987535

8. Goh G, Schmid R, Guiver K, Arpornwirat W, Chitapanarux I, Ganju V, et al. Clonal Evolutionary Analysis during HER2 Blockade in HER2-Positive Inflammatory Breast Cancer: A Phase II Open-Label Clinical Trial of Afatinib +/- Vinorelbine. PLoS Med. 2016;13(12):e1002136. doi: 10.1371/journal.pmed.1002136

9. Jeselsohn R, Buchwalter G, De Angelis C, Brown M, Schiff R. ESR1 mutations—a mechanism for acquired endocrine resistance in breast cancer. Nat Rev Clin Oncol. 2015 Oct;12(10):573–83. http://www.ncbi.nlm.nih.gov/pubmed/26122181 doi: 10.1038/nrclinonc.2015.117 26122181

Štítky
Interní lékařství

Článek vyšel v časopise

PLOS Medicine


2016 Číslo 12
Nejčtenější tento týden
Nejčtenější v tomto čísle
Kurzy Podcasty Doporučená témata Časopisy
Přihlášení
Zapomenuté heslo

Zadejte e-mailovou adresu, se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.

Přihlášení

Nemáte účet?  Registrujte se

#ADS_BOTTOM_SCRIPTS#